LOCUS       BBa_K737056             2754 bp    DNA     linear       10-May-2024
DEFINITION  YtfJ_s1s2_Gnt
ACCESSION   BBa_K737056
VERSION     BBa_K737056.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..721
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     misc_feature    44..883
                     /label=BBa_P0440
     misc_feature    894..910
                     /label=lac O1
     promoter        911..916
                     /label=-35
     misc_feature    917..933
                     /label=lac O1
     promoter        934..939
                     /label=-10
     misc_feature    892..945
                     /label=BBa_R0011
     promoter        892..945
                     /label=BBa_R0011
     misc_feature    955..1216
                     /label=BBa_K737049
     promoter        1225..1259
                     /label=BBa_J23106
     misc_feature    1272..1275
                     /label=conserved
     RBS             1268..1279
                     /label=BBa_B0034
     misc_feature    2375..2413
                     /label=LVA
     CDS             1286..2413
                     /label=lacI-LVA
     misc_feature    1286..2413
                     /label=BBa_C0012
     misc_feature    2414..2438
                     /label=Help:Barcodes
     CDS             1286..2413
                     /label=BBa_C0012
     stem_loop       2458..2501
                     /label=stem_loop
     misc_feature    2447..2526
                     /label=BBa_B0010
     terminator      2447..2526
                     /label=BBa_B0010
     stem_loop       2542..2561
                     /label=T7 TE
     misc_feature    2568..2568
                     /label=stop
     polyA_site      2562..2575
                     /label=polya
     misc_feature    2535..2575
                     /label=BBa_B0012
     terminator      2535..2575
                     /label=BBa_B0012
     misc_feature    1268..2575
                     /label=BBa_P0412
     misc_feature    2584..2602
                     /label=TetR 1
     misc_feature    2584..2637
                     /label=BBa_R0040
     promoter        2603..2608
                     /label=-35
     misc_feature    2609..2627
                     /label=TetR 2
     promoter        2626..2631
                     /label=-10
     promoter        2584..2637
                     /label=BBa_R0040
     misc_feature    2646..2754
                     /label=BBa_K737058
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag
      901 cggataacaa ttgacattgt gagcggataa caagatactg agcacatact agagcgcggt
      961 cttcagtata agcattcatt tttcatatga tttgtgcgct tgaccgcaaa ctggcatcac
     1021 acttgcggga aattcgataa atagcacata tgattaaaac tcagacccaa gtggtcggat
     1081 cacctgcata tcataagaag gaaacaccat gaccctacgc aagattctgg cactcacctg
     1141 cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa accggtcagc gagtgccgcc
     1201 gattggcatt accgattact agagtttacg gctagctcag tcctaggtat agtgctagct
     1261 actagagaaa gaggagaaat actagatggt gaatgtgaaa ccagtaacgt tatacgatgt
     1321 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca
     1381 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc
     1441 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc
     1501 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca
     1561 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc
     1621 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga
     1681 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga
     1741 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact
     1801 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt
     1861 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca
     1921 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac
     1981 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat
     2041 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc
     2101 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat
     2161 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
     2221 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac
     2281 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2341 ggcacgacag gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa actacgcttt
     2401 agtagcttaa taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata
     2461 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2521 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
     2581 gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac
     2641 tagaggtagg gtacagaggt aagatgttct atctttcaga ccttttactt cacgtaatcg
     2701 gatttggctg aatattttag ccgccccagt cagtaatgac tggggcgttt ttta
//