LOCUS       BBa_K737060             1277 bp    DNA     linear       22-Dec-2024
DEFINITION  YtfJ_Constitutive buffer RNA generator
ACCESSION   BBa_K737060
VERSION     BBa_K737060.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..721
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     misc_feature    44..883
                     /label=BBa_P0440
     misc_feature    894..910
                     /label=lac O1
     promoter        911..916
                     /label=-35
     misc_feature    917..933
                     /label=lac O1
     promoter        934..939
                     /label=-10
     misc_feature    892..945
                     /label=BBa_R0011
     promoter        892..945
                     /label=BBa_R0011
     misc_feature    955..1007
                     /label=BBa_K737057
     misc_feature    1016..1277
                     /label=BBa_K737049
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag
      901 cggataacaa ttgacattgt gagcggataa caagatactg agcacatact agagatttgg
      961 ctgaatattt tagccgcccc agtcagtaat gactggggcg ttttttatac tagagcgcgg
     1021 tcttcagtat aagcattcat ttttcatatg atttgtgcgc ttgaccgcaa actggcatca
     1081 cacttgcggg aaattcgata aatagcacat atgattaaaa ctcagaccca agtggtcgga
     1141 tcacctgcat atcataagaa ggaaacacca tgaccctacg caagattctg gcactcacct
     1201 gcctgctgtt gccgatgatg gcttccgcac atcagttcga aaccggtcag cgagtgccgc
     1261 cgattggcat taccgat
//