BBa_K737067
1
BBa_K737067
pLux OmpR hybrid promoter
2012-09-20T11:00:00Z
2015-05-08T01:13:08Z
No
Positively regulated, OmpR-controlled promoter ligated with B0034 can be used to controll Genetic circuit by light with light-dependent control element.This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
false
false
_986_
0
14291
9
It's complicated
false
No
false
Tianhe Wang
component2189702
1
BBa_R0082
component2189704
1
BBa_B0034
annotation2189702
1
BBa_R0082
range2189702
1
1
108
annotation2189704
1
BBa_B0034
range2189704
1
117
128
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_R0082
1
OmpR
Promoter (OmpR, positive)
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
NC_000193 E. coli K12
Released HQ 2013
Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
false
false
_1_
0
24
7
In stock
false
The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1.
An alternate version, BBa_R0083, cuts out the C2 and C3 sites.
The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
true
Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
annotation301167
1
-10
range301167
1
98
103
annotation301166
1
-35
range301166
1
75
80
annotation301154
1
C1 OmpR
range301154
1
13
30
annotation301155
1
C2 OmpR
range301155
1
34
51
annotation301156
1
C3 OmpR
range301156
1
54
71
BBa_R0082_sequence
1
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K737067_sequence
1
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z