LOCUS       BBa_K737069              984 bp    DNA     linear       22-Dec-2024
DEFINITION  K216005+E0240
ACCESSION   BBa_K737069
VERSION     BBa_K737069.1  
FEATURES             Location/Qualifiers
     misc_feature    50..65
                     /label=NarL-binding region
     misc_feature    58..80
                     /label=NsrR-binding region
     misc_feature    66..71
                     /label=promoter -35 region
     misc_feature    89..94
                     /label=promoter -10 region
     promoter        1..100
                     /label=BBa_K216005
     RBS             109..121
                     /label=RBS-3\Weak
     RBS             115..118
                     /label=RBS
     misc_feature    109..121
                     /label=BBa_B0032
     RBS             109..121
                     /label=BBa_B0032
     CDS             128..847
                     /label=GFP protein
     CDS             128..847
                     /label=BBa_E0040
     stem_loop       867..910
                     /label=stem_loop
     misc_feature    856..935
                     /label=BBa_B0010
     terminator      856..935
                     /label=BBa_B0010
     stem_loop       951..970
                     /label=T7 TE
     misc_feature    977..977
                     /label=stop
     polyA_site      971..984
                     /label=polya
     misc_feature    944..984
                     /label=BBa_B0012
     terminator      944..984
                     /label=BBa_B0012
     misc_feature    109..984
                     /label=BBa_E0240
ORIGIN
        1 ttcccatcta taatcctccc tgattcttcg ctgatatggt gctaaaaagt aaccaataaa
       61 tggtatttaa aatgcaaatt atcaggcgta ccctgaaacg tactagagtc acacaggaaa
      121 gtactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      181 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      241 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
      301 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
      361 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      421 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
      481 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
      541 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
      601 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
      661 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
      721 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
      781 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
      841 ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      901 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      961 cgggtgggcc tttctgcgtt tata
//