LOCUS       BBa_K740001              972 bp    DNA     linear       25-Nov-2024
DEFINITION  K740000 followed by rfp
ACCESSION   BBa_K740001
VERSION     BBa_K740001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..34
                     /label=loxP
     promoter        35..69
                     /label=J23115
     misc_feature    70..103
                     /label=loxP
     promoter        1..103
                     /label=BBa_K740000
     misc_feature    116..119
                     /label=conserved
     RBS             112..123
                     /label=BBa_B0034
     CDS             130..804
                     /label=mrfp1
     misc_feature    811..835
                     /label=Help:Barcodes
     CDS             130..835
                     /label=BBa_E1010
     misc_feature    112..835
                     /label=BBa_K093005
     stem_loop       855..898
                     /label=stem_loop
     misc_feature    844..923
                     /label=BBa_B0010
     terminator      844..923
                     /label=BBa_B0010
     stem_loop       939..958
                     /label=T7 TE
     misc_feature    965..965
                     /label=stop
     polyA_site      959..972
                     /label=polya
     misc_feature    932..972
                     /label=BBa_B0012
     terminator      932..972
                     /label=BBa_B0012
     terminator      844..972
                     /label=BBa_B0015
ORIGIN
        1 ataacttcgt ataatgtatg ctatacgaag ttattttata gctagctcag cccttggtac
       61 aatgctagca taacttcgta tagcatacat tatacgaagt tattactaga gaaagaggag
      121 aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt
      181 atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg
      241 tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct
      301 tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct
      361 gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg
      421 aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag
      481 ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag
      541 aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg
      601 aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt
      661 aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc
      721 aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct
      781 gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta
      841 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      901 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      961 tctgcgttta ta
//