LOCUS       BBa_K740003             1858 bp    DNA     linear       23-May-2024
DEFINITION  promoter with loxP sites that can switch the reporter
ACCESSION   BBa_K740003
VERSION     BBa_K740003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..878
                     /label=BBa_K740002
     misc_feature    887..920
                     /label=loxP
     promoter        921..955
                     /label=J23115
     misc_feature    956..989
                     /label=loxP
     promoter        887..989
                     /label=BBa_K740000
     misc_feature    1002..1005
                     /label=conserved
     RBS             998..1009
                     /label=BBa_B0034
     CDS             1016..1690
                     /label=mrfp1
     misc_feature    1697..1721
                     /label=Help:Barcodes
     CDS             1016..1721
                     /label=BBa_E1010
     misc_feature    998..1721
                     /label=BBa_K093005
     stem_loop       1741..1784
                     /label=stem_loop
     misc_feature    1730..1809
                     /label=BBa_B0010
     terminator      1730..1809
                     /label=BBa_B0010
     stem_loop       1825..1844
                     /label=T7 TE
     misc_feature    1851..1851
                     /label=stop
     polyA_site      1845..1858
                     /label=polya
     misc_feature    1818..1858
                     /label=BBa_B0012
     terminator      1818..1858
                     /label=BBa_B0012
     terminator      1730..1858
                     /label=BBa_B0015
     misc_feature    887..1858
                     /label=BBa_K740001
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggc tctagtatta ttacttgtac agctcgtcca tgccgagagt gatcccggcg
      181 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
      241 gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      301 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
      361 ttgaagttgg ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtggctg
      421 atgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
      481 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
      541 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
      601 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccc
      661 caggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
      721 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
      781 ttgtggccgt tcacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
      841 tcctcgccct tgctcaccat ctagtatttc tcctctttta ctagagataa cttcgtataa
      901 tgtatgctat acgaagttat tttatagcta gctcagccct tggtacaatg ctagcataac
      961 ttcgtatagc atacattata cgaagttatt actagagaaa gaggagaaat actagatggc
     1021 ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
     1081 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
     1141 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
     1201 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
     1261 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg
     1321 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
     1381 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
     1441 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa
     1501 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
     1561 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
     1621 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
     1681 caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa
     1741 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1801 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//