LOCUS       BBa_K741004             2111 bp    DNA     linear       24-Jan-2026
DEFINITION  plac-RBS-cI-T-pRM-RBS-GFP-T
ACCESSION   BBa_K741004
VERSION     BBa_K741004.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             230..937
                     /label=cI lambda
     misc_feature    938..970
                     /label=LVA
     misc_feature    977..1001
                     /label=Help:Barcodes
     CDS             227..976
                     /label=BBa_C0051
     stem_loop       1021..1064
                     /label=stem_loop
     misc_feature    1010..1089
                     /label=BBa_B0010
     terminator      1010..1089
                     /label=BBa_B0010
     stem_loop       1105..1124
                     /label=T7 TE
     misc_feature    1131..1131
                     /label=stop
     polyA_site      1125..1138
                     /label=polya
     misc_feature    1098..1138
                     /label=BBa_B0012
     terminator      1098..1138
                     /label=BBa_B0012
     misc_feature    209..1138
                     /label=BBa_P0451
     misc_feature    1155..1171
                     /label=OR1
     misc_feature    1179..1195
                     /label=OR2
     promoter        1194..1199
                     /label=-35
     misc_feature    1205..1211
                     /label=mutate to obliterate OR3
     promoter        1217..1222
                     /label=-10
     promoter        1147..1228
                     /label=BBa_I12007
     misc_feature    1241..1244
                     /label=conserved
     RBS             1237..1248
                     /label=BBa_B0034
     CDS             1255..1974
                     /label=GFP protein
     CDS             1255..1974
                     /label=BBa_E0040
     stem_loop       1994..2037
                     /label=stem_loop
     misc_feature    1983..2062
                     /label=BBa_B0010
     terminator      1983..2062
                     /label=BBa_B0010
     stem_loop       2078..2097
                     /label=T7 TE
     misc_feature    2104..2104
                     /label=stop
     polyA_site      2098..2111
                     /label=polya
     misc_feature    2071..2111
                     /label=BBa_B0012
     terminator      2071..2111
                     /label=BBa_B0012
     misc_feature    1237..2111
                     /label=BBa_I13504
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gcacaaaaaa
      241 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      301 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      361 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      421 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      481 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      541 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      601 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      661 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      721 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      781 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      841 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      901 agttatcgct agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc
      961 tttagtagct taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa
     1021 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1081 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
     1141 ctagaggcaa ccattatcac cgccagaggt aaaatagtca acacgcacgg tgttagatat
     1201 ttataaatag tggtgataga tttaacgtta ctagagaaag aggagaaata ctagatgcgt
     1261 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     1321 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     1381 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
     1441 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac
     1501 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
     1561 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
     1621 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
     1681 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
     1741 gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
     1801 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
     1861 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
     1921 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag
     1981 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     2041 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     2101 ctgcgtttat a
//