LOCUS       BBa_K741007             1296 bp    DNA     linear       29-Jun-2024
DEFINITION  plac-RBS-crogfp-T
ACCESSION   BBa_K741007
VERSION     BBa_K741007.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1159
                     /label=BBa_K741005
     stem_loop       1179..1222
                     /label=stem_loop
     misc_feature    1168..1247
                     /label=BBa_B0010
     terminator      1168..1247
                     /label=BBa_B0010
     stem_loop       1263..1282
                     /label=T7 TE
     misc_feature    1289..1289
                     /label=stop
     polyA_site      1283..1296
                     /label=polya
     misc_feature    1256..1296
                     /label=BBa_B0012
     terminator      1256..1296
                     /label=BBa_B0012
     terminator      1168..1296
                     /label=BBa_B0015
     misc_feature    209..1296
                     /label=BBa_K741006
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg aacaacgcat
      241 aaccctgaaa gattatgcaa tgcgctttgg gcaaaccaag acagctaaag atctcggcgt
      301 atatcaaagc gcgatcaaca aggccattca tgcaggccga aagatttttt taactataaa
      361 cgctgatgga agcgtttatg cggaagaggt aaagcccttc ccgagtaaca aaaaaacaac
      421 agcataaggt ggttctggta tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
      481 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      541 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      601 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      661 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      721 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      781 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      841 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      901 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      961 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     1021 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     1081 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     1141 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1201 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1261 ctggctcacc ttcgggtggg cctttctgcg tttata
//