LOCUS       BBa_K741010              546 bp    DNA     linear       29-Jun-2024
DEFINITION  plac-anticro-T
ACCESSION   BBa_K741010
VERSION     BBa_K741010.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..409
                     /label=BBa_K741000
     stem_loop       429..472
                     /label=stem_loop
     misc_feature    418..497
                     /label=BBa_B0010
     terminator      418..497
                     /label=BBa_B0010
     stem_loop       513..532
                     /label=T7 TE
     misc_feature    539..539
                     /label=stop
     polyA_site      533..546
                     /label=polya
     misc_feature    506..546
                     /label=BBa_B0012
     terminator      506..546
                     /label=BBa_B0012
     terminator      418..546
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtt atgctgttgt ttttttgtta ctcgggaagg
      241 gctttacctc ttccgcataa acgcttccat cagcgtttat agttaaaaaa atctttcggc
      301 ctgcatgaat ggccttgttg atcgcgcttt gatatacgcc gagatcttta gctgtcttgg
      361 tttgcccaaa gcgcattgca taatctttca gggttatgcg ttgttccatt actagagcca
      421 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      481 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      541 tttata
//