LOCUS       BBa_K741012             1685 bp    DNA     linear       22-Nov-2024
DEFINITION  plac-anticro-T -pCon(0.856)-RBS-crogfp-T
ACCESSION   BBa_K741012
VERSION     BBa_K741012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23102
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..994
                     /label=BBa_K741005
     stem_loop       1014..1057
                     /label=stem_loop
     misc_feature    1003..1082
                     /label=BBa_B0010
     terminator      1003..1082
                     /label=BBa_B0010
     stem_loop       1098..1117
                     /label=T7 TE
     misc_feature    1124..1124
                     /label=stop
     polyA_site      1118..1131
                     /label=polya
     misc_feature    1091..1131
                     /label=BBa_B0012
     terminator      1091..1131
                     /label=BBa_B0012
     terminator      1003..1131
                     /label=BBa_B0015
     misc_feature    44..1131
                     /label=BBa_K741006
     misc_feature    1..1131
                     /label=BBa_K741009
     CDS             1140..1227
                     /label=end of LacI coding region (inactive)
     misc_feature    1140..1339
                     /label=BBa_R0010
     misc_feature    1228..1265
                     /label=CAP binding site
     promoter        1276..1281
                     /label=-35
     promoter        1300..1305
                     /label=-10
     misc_feature    1305..1339
                     /label=LacI binding site
     misc_feature    1312..1312
                     /label=start
     promoter        1140..1339
                     /label=BBa_R0010
     misc_feature    1348..1548
                     /label=BBa_K741000
     stem_loop       1568..1611
                     /label=stem_loop
     misc_feature    1557..1636
                     /label=BBa_B0010
     terminator      1557..1636
                     /label=BBa_B0010
     stem_loop       1652..1671
                     /label=T7 TE
     misc_feature    1678..1678
                     /label=stop
     polyA_site      1672..1685
                     /label=polya
     misc_feature    1645..1685
                     /label=BBa_B0012
     terminator      1645..1685
                     /label=BBa_B0012
     terminator      1557..1685
                     /label=BBa_B0015
     misc_feature    1140..1685
                     /label=BBa_K741010
ORIGIN
        1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagaaagagg agaaatacta
       61 gatggaacaa cgcataaccc tgaaagatta tgcaatgcgc tttgggcaaa ccaagacagc
      121 taaagatctc ggcgtatatc aaagcgcgat caacaaggcc attcatgcag gccgaaagat
      181 ttttttaact ataaacgctg atggaagcgt ttatgcggaa gaggtaaagc ccttcccgag
      241 taacaaaaaa acaacagcat aaggtggttc tggtatgcgt aaaggagaag aacttttcac
      301 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      361 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
      421 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca
      481 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc
      541 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg
      601 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
      661 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
      721 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca
      781 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg
      841 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
      901 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
      961 tacacatggc atggatgaac tatacaaata ataatactag agccaggcat caaataaaac
     1021 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1081 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagc
     1141 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1201 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1261 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1321 cggataacaa tttcacacat actagagtta tgctgttgtt tttttgttac tcgggaaggg
     1381 ctttacctct tccgcataaa cgcttccatc agcgtttata gttaaaaaaa tctttcggcc
     1441 tgcatgaatg gccttgttga tcgcgctttg atatacgccg agatctttag ctgtcttggt
     1501 ttgcccaaag cgcattgcat aatctttcag ggttatgcgt tgttccatta ctagagccag
     1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1621 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1681 ttata
//