LOCUS       BBa_K741014              389 bp    DNA     linear       22-Dec-2024
DEFINITION  pRM-RBS-lysis-T
ACCESSION   BBa_K741014
VERSION     BBa_K741014.1  
FEATURES             Location/Qualifiers
     misc_feature    9..25
                     /label=OR1
     misc_feature    33..49
                     /label=OR2
     promoter        48..53
                     /label=-35
     misc_feature    59..65
                     /label=mutate to obliterate OR3
     promoter        71..76
                     /label=-10
     promoter        1..82
                     /label=BBa_I12007
     misc_feature    95..98
                     /label=conserved
     RBS             91..102
                     /label=BBa_B0034
     CDS             109..252
                     /label=BBa_K117000
     stem_loop       272..315
                     /label=stem_loop
     misc_feature    261..340
                     /label=BBa_B0010
     terminator      261..340
                     /label=BBa_B0010
     stem_loop       356..375
                     /label=T7 TE
     misc_feature    382..382
                     /label=stop
     polyA_site      376..389
                     /label=polya
     misc_feature    349..389
                     /label=BBa_B0012
     terminator      349..389
                     /label=BBa_B0012
     terminator      261..389
                     /label=BBa_B0015
     misc_feature    91..389
                     /label=BBa_K741013
ORIGIN
        1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa
       61 atagtggtga tagatttaac gttactagag aaagaggaga aatactagat gaaaaaaata
      121 acagggatta ttttattgct tcttgcagcc attattcttg ctgcatgtca ggcaaactat
      181 atccgtgatg ttcagggcgg gacagtatca ccgtcgtcaa ctgctgaact gaccggagtg
      241 gaaacgcagt aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
      301 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      361 accttcgggt gggcctttct gcgtttata
//