LOCUS BBa_K741018 2713 bp DNA linear 22-Nov-2024 DEFINITION plac-RBS-cI-T-pRM-anticro-T-pCon(0.856)-RBS-crogfp-T ACCESSION BBa_K741018 VERSION BBa_K741018.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 230..937 /label=cI lambda misc_feature 938..970 /label=LVA misc_feature 977..1001 /label=Help:Barcodes CDS 227..976 /label=BBa_C0051 stem_loop 1021..1064 /label=stem_loop misc_feature 1010..1089 /label=BBa_B0010 terminator 1010..1089 /label=BBa_B0010 stem_loop 1105..1124 /label=T7 TE misc_feature 1131..1131 /label=stop polyA_site 1125..1138 /label=polya misc_feature 1098..1138 /label=BBa_B0012 terminator 1098..1138 /label=BBa_B0012 misc_feature 209..1138 /label=BBa_P0451 misc_feature 1..1138 /label=BBa_K741001 misc_feature 1155..1171 /label=OR1 misc_feature 1179..1195 /label=OR2 promoter 1194..1199 /label=-35 misc_feature 1205..1211 /label=mutate to obliterate OR3 promoter 1217..1222 /label=-10 promoter 1147..1228 /label=BBa_I12007 misc_feature 1237..1437 /label=BBa_K741000 stem_loop 1457..1500 /label=stem_loop misc_feature 1446..1525 /label=BBa_B0010 terminator 1446..1525 /label=BBa_B0010 stem_loop 1541..1560 /label=T7 TE misc_feature 1567..1567 /label=stop polyA_site 1561..1574 /label=polya misc_feature 1534..1574 /label=BBa_B0012 terminator 1534..1574 /label=BBa_B0012 terminator 1446..1574 /label=BBa_B0015 misc_feature 1147..1574 /label=BBa_K741016 promoter 1583..1617 /label=BBa_J23102 misc_feature 1630..1633 /label=conserved RBS 1626..1637 /label=BBa_B0034 CDS 1644..2576 /label=BBa_K741005 stem_loop 2596..2639 /label=stem_loop misc_feature 2585..2664 /label=BBa_B0010 terminator 2585..2664 /label=BBa_B0010 stem_loop 2680..2699 /label=T7 TE misc_feature 2706..2706 /label=stop polyA_site 2700..2713 /label=polya misc_feature 2673..2713 /label=BBa_B0012 terminator 2673..2713 /label=BBa_B0012 terminator 2585..2713 /label=BBa_B0015 misc_feature 1626..2713 /label=BBa_K741006 misc_feature 1583..2713 /label=BBa_K741009 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gcacaaaaaa 241 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa 301 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca 361 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt 421 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat 481 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc 541 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg 601 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga 661 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat 721 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact 781 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca 841 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa 901 agttatcgct agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc 961 tttagtagct taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa 1021 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 1081 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 1141 ctagaggcaa ccattatcac cgccagaggt aaaatagtca acacgcacgg tgttagatat 1201 ttataaatag tggtgataga tttaacgtta ctagagttat gctgttgttt ttttgttact 1261 cgggaagggc tttacctctt ccgcataaac gcttccatca gcgtttatag ttaaaaaaat 1321 ctttcggcct gcatgaatgg ccttgttgat cgcgctttga tatacgccga gatctttagc 1381 tgtcttggtt tgcccaaagc gcattgcata atctttcagg gttatgcgtt gttccattac 1441 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 1501 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 1561 tttctgcgtt tatatactag agttgacagc tagctcagtc ctaggtactg tgctagctac 1621 tagagaaaga ggagaaatac tagatggaac aacgcataac cctgaaagat tatgcaatgc 1681 gctttgggca aaccaagaca gctaaagatc tcggcgtata tcaaagcgcg atcaacaagg 1741 ccattcatgc aggccgaaag atttttttaa ctataaacgc tgatggaagc gtttatgcgg 1801 aagaggtaaa gcccttcccg agtaacaaaa aaacaacagc ataaggtggt tctggtatgc 1861 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg 1921 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac 1981 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca 2041 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg 2101 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag 2161 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata 2221 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg 2281 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca 2341 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt 2401 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt 2461 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg 2521 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataatact 2581 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 2641 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 2701 ttctgcgttt ata //