LOCUS       BBa_K741019              825 bp    DNA     linear       23-Dec-2024
DEFINITION  pRM-anticro-T-pRM-RBS-lysis-T
ACCESSION   BBa_K741019
VERSION     BBa_K741019.1  
FEATURES             Location/Qualifiers
     misc_feature    9..25
                     /label=OR1
     misc_feature    33..49
                     /label=OR2
     promoter        48..53
                     /label=-35
     misc_feature    59..65
                     /label=mutate to obliterate OR3
     promoter        71..76
                     /label=-10
     promoter        1..82
                     /label=BBa_I12007
     misc_feature    91..291
                     /label=BBa_K741000
     stem_loop       311..354
                     /label=stem_loop
     misc_feature    300..379
                     /label=BBa_B0010
     terminator      300..379
                     /label=BBa_B0010
     stem_loop       395..414
                     /label=T7 TE
     misc_feature    421..421
                     /label=stop
     polyA_site      415..428
                     /label=polya
     misc_feature    388..428
                     /label=BBa_B0012
     terminator      388..428
                     /label=BBa_B0012
     terminator      300..428
                     /label=BBa_B0015
     misc_feature    1..428
                     /label=BBa_K741016
     misc_feature    445..461
                     /label=OR1
     misc_feature    469..485
                     /label=OR2
     promoter        484..489
                     /label=-35
     misc_feature    495..501
                     /label=mutate to obliterate OR3
     promoter        507..512
                     /label=-10
     promoter        437..518
                     /label=BBa_I12007
     misc_feature    531..534
                     /label=conserved
     RBS             527..538
                     /label=BBa_B0034
     CDS             545..688
                     /label=BBa_K117000
     stem_loop       708..751
                     /label=stem_loop
     misc_feature    697..776
                     /label=BBa_B0010
     terminator      697..776
                     /label=BBa_B0010
     stem_loop       792..811
                     /label=T7 TE
     misc_feature    818..818
                     /label=stop
     polyA_site      812..825
                     /label=polya
     misc_feature    785..825
                     /label=BBa_B0012
     terminator      785..825
                     /label=BBa_B0012
     terminator      697..825
                     /label=BBa_B0015
     misc_feature    527..825
                     /label=BBa_K741013
     misc_feature    437..825
                     /label=BBa_K741014
ORIGIN
        1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa
       61 atagtggtga tagatttaac gttactagag ttatgctgtt gtttttttgt tactcgggaa
      121 gggctttacc tcttccgcat aaacgcttcc atcagcgttt atagttaaaa aaatctttcg
      181 gcctgcatga atggccttgt tgatcgcgct ttgatatacg ccgagatctt tagctgtctt
      241 ggtttgccca aagcgcattg cataatcttt cagggttatg cgttgttcca ttactagagc
      301 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      361 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
      421 cgtttatata ctagaggcaa ccattatcac cgccagaggt aaaatagtca acacgcacgg
      481 tgttagatat ttataaatag tggtgataga tttaacgtta ctagagaaag aggagaaata
      541 ctagatgaaa aaaataacag ggattatttt attgcttctt gcagccatta ttcttgctgc
      601 atgtcaggca aactatatcc gtgatgttca gggcgggaca gtatcaccgt cgtcaactgc
      661 tgaactgacc ggagtggaaa cgcagtaata ctagagccag gcatcaaata aaacgaaagg
      721 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      781 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//