LOCUS       BBa_K741020             2277 bp    DNA     linear       22-Nov-2024
DEFINITION  plac-RBS-cI-T-pCon(0.856)-RBS-crogfp-T
ACCESSION   BBa_K741020
VERSION     BBa_K741020.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             230..937
                     /label=cI lambda
     misc_feature    938..970
                     /label=LVA
     misc_feature    977..1001
                     /label=Help:Barcodes
     CDS             227..976
                     /label=BBa_C0051
     stem_loop       1021..1064
                     /label=stem_loop
     misc_feature    1010..1089
                     /label=BBa_B0010
     terminator      1010..1089
                     /label=BBa_B0010
     stem_loop       1105..1124
                     /label=T7 TE
     misc_feature    1131..1131
                     /label=stop
     polyA_site      1125..1138
                     /label=polya
     misc_feature    1098..1138
                     /label=BBa_B0012
     terminator      1098..1138
                     /label=BBa_B0012
     misc_feature    209..1138
                     /label=BBa_P0451
     misc_feature    1..1138
                     /label=BBa_K741001
     promoter        1147..1181
                     /label=BBa_J23102
     misc_feature    1194..1197
                     /label=conserved
     RBS             1190..1201
                     /label=BBa_B0034
     CDS             1208..2140
                     /label=BBa_K741005
     stem_loop       2160..2203
                     /label=stem_loop
     misc_feature    2149..2228
                     /label=BBa_B0010
     terminator      2149..2228
                     /label=BBa_B0010
     stem_loop       2244..2263
                     /label=T7 TE
     misc_feature    2270..2270
                     /label=stop
     polyA_site      2264..2277
                     /label=polya
     misc_feature    2237..2277
                     /label=BBa_B0012
     terminator      2237..2277
                     /label=BBa_B0012
     terminator      2149..2277
                     /label=BBa_B0015
     misc_feature    1190..2277
                     /label=BBa_K741006
     misc_feature    1147..2277
                     /label=BBa_K741009
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gcacaaaaaa
      241 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      301 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      361 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      421 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      481 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      541 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      601 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      661 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      721 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      781 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      841 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      901 agttatcgct agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc
      961 tttagtagct taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa
     1021 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1081 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
     1141 ctagagttga cagctagctc agtcctaggt actgtgctag ctactagaga aagaggagaa
     1201 atactagatg gaacaacgca taaccctgaa agattatgca atgcgctttg ggcaaaccaa
     1261 gacagctaaa gatctcggcg tatatcaaag cgcgatcaac aaggccattc atgcaggccg
     1321 aaagattttt ttaactataa acgctgatgg aagcgtttat gcggaagagg taaagccctt
     1381 cccgagtaac aaaaaaacaa cagcataagg tggttctggt atgcgtaaag gagaagaact
     1441 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
     1501 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
     1561 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
     1621 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
     1681 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
     1741 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
     1801 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
     1861 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
     1921 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
     1981 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
     2041 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
     2101 tgggattaca catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa
     2161 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2221 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//