##gff-version 3
##sequence-region BBa_K741020 1 2277
BBa_K741020	.	engineered_region	1147	2277	.	+	0	ID=BBa_K741009;Name=BBa_K741009
BBa_K741020	.	promoter	1147	1181	.	+	0	ID=BBa_J23102;Name=BBa_J23102;Parent=BBa_K741009
BBa_K741020	.	engineered_region	1190	2277	.	+	0	ID=BBa_K741006;Name=BBa_K741006;Parent=BBa_K741009
BBa_K741020	.	CDS	1208	2140	.	+	0	ID=BBa_K741005;Name=BBa_K741005;Parent=BBa_K741006
BBa_K741020	.	ribosome_entry_site	1190	1201	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K741006
BBa_K741020	.	conserved_region	1194	1197	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K741020	.	terminator	2149	2277	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K741006
BBa_K741020	.	terminator	2149	2228	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K741020	.	engineered_region	2149	2228	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K741020	.	stem_loop	2160	2203	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K741020	.	terminator	2237	2277	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K741020	.	engineered_region	2237	2277	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K741020	.	stop_codon	2270	2270	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K741020	.	stem_loop	2244	2263	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K741020	.	polyA_site	2264	2277	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K741020	.	engineered_region	1	1138	.	+	0	ID=BBa_K741001;Name=BBa_K741001
BBa_K741020	.	engineered_region	209	1138	.	+	0	ID=BBa_P0451;Name=BBa_P0451;Parent=BBa_K741001
BBa_K741020	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0451
BBa_K741020	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K741020	.	terminator	1010	1089	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0451
BBa_K741020	.	engineered_region	1010	1089	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K741020	.	stem_loop	1021	1064	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K741020	.	terminator	1098	1138	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0451
BBa_K741020	.	engineered_region	1098	1138	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K741020	.	stop_codon	1131	1131	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K741020	.	stem_loop	1105	1124	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K741020	.	polyA_site	1125	1138	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K741020	.	CDS	227	976	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0451
BBa_K741020	.	tag	938	970	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K741020	.	CDS	230	937	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K741020	.	engineered_tag	977	1001	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K741020	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K741001
BBa_K741020	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K741020	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K741020	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K741020	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K741020	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K741020	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K741020	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
>BBa_K741020
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgagcacaaa
aaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaactt
ggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatg
cattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccag
agaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcat
gttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaa
aagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcc
tgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgat
gagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcc
catgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacga
cgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtactgtgctagctact
agagaaagaggagaaatactagatggaacaacgcataaccctgaaagattatgcaatgcgctttgggcaaaccaagaca
gctaaagatctcggcgtatatcaaagcgcgatcaacaaggccattcatgcaggccgaaagatttttttaactataaacg
ctgatggaagcgtttatgcggaagaggtaaagcccttcccgagtaacaaaaaaacaacagcataaggtggttctggtat
gcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattt
tctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactac
ctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaaca
gcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactac
aagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaag
atggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaa
tggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaat
actccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatccca
acgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaa
ataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata