LOCUS       BBa_K743005             3239 bp    DNA     linear       28-Jun-2024
DEFINITION  psb1C3_IntK plasmid with RFP under psbA2 promoter
ACCESSION   BBa_K743005
VERSION     BBa_K743005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..522
                     /label=RS1 recombination site
     misc_feature    1..522
                     /label=BBa_K743000
     promoter        531..568
                     /label=psbA2 promoter
     RBS             601..610
                     /label=putative rbs
     misc_feature    533..538
                     /label=-35 box
     misc_feature    557..562
                     /label=-10 box
     misc_feature    569..569
                     /label=+1 Transcription Start Point
     promoter        531..617
                     /label=BBa_K390017
     CDS             624..1298
                     /label=mrfp1
     misc_feature    1305..1329
                     /label=Help:Barcodes
     CDS             624..1304
                     /label=BBa_E1010
     stem_loop       1349..1392
                     /label=stem_loop
     misc_feature    1338..1417
                     /label=BBa_B0010
     terminator      1338..1417
                     /label=BBa_B0010
     stem_loop       1433..1452
                     /label=T7 TE
     misc_feature    1459..1459
                     /label=stop
     polyA_site      1453..1466
                     /label=polya
     misc_feature    1426..1466
                     /label=BBa_B0012
     terminator      1426..1466
                     /label=BBa_B0012
     terminator      1338..1466
                     /label=BBa_B0015
     CDS             1623..2441
                     /label=kanamycin resistance
     misc_feature    1475..2441
                     /label=BBa_P1003
     stem_loop       2457..2476
                     /label=T7 TE
     misc_feature    2483..2483
                     /label=stop
     polyA_site      2477..2490
                     /label=polya
     misc_feature    2450..2490
                     /label=BBa_B0012
     terminator      2450..2490
                     /label=BBa_B0012
     stem_loop       2511..2533
                     /label=stem_loop
     misc_feature    2499..2544
                     /label=BBa_B0011
     terminator      2499..2544
                     /label=BBa_B0011
     misc_feature    1475..2544
                     /label=BBa_K292003
     misc_feature    2553..3239
                     /label=RS2
     misc_feature    2553..3239
                     /label=BBa_K743001
ORIGIN
        1 tcccagcctc tcaaccaccg atattgggcc cctagctacc cctgatattt tggcggacat
       61 tgttgcgcaa gtagaacaaa ccattgctgc tggagcccac tgtcgttgtg gcggccaagc
      121 cctagaccaa ccgggaaatt actatcctcc caccctgctc accgacgttc cccccaacgc
      181 ccctacctac cgtcaggaat tttttggccc cgtggccctc ggatttactg tcgataattt
      241 agaggaggcg atcgccttgg ccaatgacat tccctttggg ttgggggcca gtgcatggac
      301 aactaacccg gaaaatcaac aaaaactaat ccggggcata gaagccgggg ctgtattcat
      361 taacggtatg actaaatctg acccccgcat tccctttgga ggcatcaagc gctctggctt
      421 tgggcgggaa cttggccgca tgggcatttt agaatttgtc aatgctaaaa ccgtttggat
      481 tgcttagtcc gccaccattt taaaattatc gctcttctat tctactagag gctttacaaa
      541 actctcatta atcctttaga ctaagtttag tcagttccaa tctgaacatc gacaaataca
      601 taaggaatta taaccaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc
      661 gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag
      721 gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc
      781 cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg
      841 ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat
      901 gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc
      961 tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg
     1021 gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg
     1081 aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact
     1141 acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt
     1201 acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac
     1261 agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt
     1321 gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1381 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1441 ttcgggtggg cctttctgcg tttatatact agagctgatc cttcaactca gcaaaagttc
     1501 gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
     1561 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     1621 ttatgagcca tattcaacgg gaaacgtctt gctcccgtcc gcgcttaaac tccaacatgg
     1681 acgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     1741 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     1801 gcgttgccaa tgatgttaca gatgagatgg tccgtctcaa ctggctgacg gagtttatgc
     1861 ctctcccgac catcaagcat tttatccgta ctcctgatga tgcgtggtta ctcaccaccg
     1921 cgattcctgg gaaaacagcc ttccaggtat tagaagaata tcctgattca ggtgaaaata
     1981 ttgttgatgc gctggccgtg ttcctgcgcc ggttacattc gattcctgtt tgtaattgtc
     2041 cttttaacag cgatcgtgta tttcgtcttg ctcaggcgca atcacgcatg aataacggtt
     2101 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     2161 aagaaatgca caagctcttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     2221 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacggg
     2281 tcggaatcgc agaccgttac caggaccttg ccattctttg gaactgcctc ggtgagtttt
     2341 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     2401 aattgcagtt tcatttgatg ctcgatgagt ttttctaata atactagagt cacactggct
     2461 caccttcggg tgggcctttc tgcgtttata tactagagag agaatataaa aagccagatt
     2521 attaatccgg cttttttatt attttactag agagtgtagg cggtaaagtc tatgggaact
     2581 tccagtttaa gggctgtccc gttaaatatg gcttcgagtt tttgaagcat aattttctaa
     2641 cgtctgtgga tttttttaag catagcttga ccttctgttg gtcgaattct agggattttc
     2701 atccatattc ggttcatagg gcagctcaat ggtgaacatt gctcccccag tttcatggtt
     2761 agctgcggtt aaatttcccc tgtgggccag gacaatttcc ttggcgatcg ccaaacccag
     2821 gccactgcca tggcgttgac tatcgggatg taggcgagca cgggaactat caccccgata
     2881 aagtcgctca aaaatgtagg gtaaatccct tggttgaaat cctaggcctt gatccctaat
     2941 aatcatggtg atcccttctg tgccttgatg accctgcaca aaaatagtcc cttccggtgg
     3001 gctatatttc agcgtattgt ccaaaatatt catcaatacc tgcatcagac gatcgccatc
     3061 cccctctagg ttcaacttgg ttggcccttg gtaatccagg gtgatatttt ttaccacggc
     3121 gatcggggtt aatcgctccc aggtaatggc gagtaaatgg cgtaaattaa tcggttctgg
     3181 ctcgaggtaa agattgggat tagcggttat ttgggttaaa tggagccaac tttccacca
//