psb1C3_Int
type: ComponentDefinition
persistent identity: BBa K743005
display id: BBa_K743005
version: 1
was generated by: iGEM to SBOL conversion
description: psb1C3_IntK plasmid with RFP under psbA2 promoter
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:09Z
mutable provenance: RS1 and RS2 were obtained from Synechocystis genomic DNA, both the kanamycin resistance and the double terminator are from the registry of standard biological parts.
owned by: james
owned by: myers
mutable description: This plasmid allows the integration of a Kanamycin resistance casette followed by a double terminator in to Synechocystis chromosome. The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly. It was used by UC_Chile 2012 team to succesfully transform Synechocystis [http://2012.igem.org/Team:UC_Chile]
discontinued: true
dominant: false
experience: experience/Works
group u list: _994_
m user id: 0
owner id: 11584
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
star: false
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_K292003
component: BBa_B0015
component: BBa_E1010
component: BBa_K390017
component: BBa_K743001
component: BBa_K743000
sequence annotation: BBa_K743000
sequence annotation: BBa_K390017
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence annotation: BBa_K292003
sequence annotation: BBa_K743001
sequence: https://synbiohub.org/public/igem/BBa_K743005_sequence/1

BBa_K292003
type: Component
persistent identity: component2193935
display id: component2193935
version: 1
top level: psb1C3_Int
definition: BBa_K292003
access: public

BBa_B0015
type: Component
persistent identity: component2193926
display id: component2193926
version: 1
top level: psb1C3_Int
definition: BBa_B0015
access: public

BBa_E1010
type: Component
persistent identity: component2193918
display id: component2193918
version: 1
top level: psb1C3_Int
definition: mRFP1
access: public

BBa_K390017
type: Component
persistent identity: component2193916
display id: component2193916
version: 1
top level: psb1C3_Int
definition: BBa_K390017
access: public

BBa_K743001
type: Component
persistent identity: component2193937
display id: component2193937
version: 1
top level: psb1C3_Int
definition: RS2
access: public

BBa_K743000
type: Component
persistent identity: component2193910
display id: component2193910
version: 1
top level: psb1C3_Int
definition: RS1
access: public

BBa_K743000
type: SequenceAnnotation
persistent identity: annotation2193910
display id: annotation2193910
version: 1
top level: psb1C3_Int
location: https://synbiohub.org/public/igem/BBa_K743005/annotation2193910/range2193910/1
component: BBa_K743000

https://synbiohub.org/public/igem/BBa_K743005/annotation2193910/range2193910/1
type: Range
persistent identity: range2193910
display id: range2193910
version: 1
top level: psb1C3_Int
direction: direction/reverse
start: 1
end: 522
orientation: inline

BBa_K390017
type: SequenceAnnotation
persistent identity: annotation2193916
display id: annotation2193916
version: 1
top level: psb1C3_Int
location: https://synbiohub.org/public/igem/BBa_K743005/annotation2193916/range2193916/1
component: BBa_K390017

https://synbiohub.org/public/igem/BBa_K743005/annotation2193916/range2193916/1
type: Range
persistent identity: range2193916
display id: range2193916
version: 1
top level: psb1C3_Int
direction: direction/reverse
start: 531
end: 617
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2193918
display id: annotation2193918
version: 1
top level: psb1C3_Int
location: https://synbiohub.org/public/igem/BBa_K743005/annotation2193918/range2193918/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K743005/annotation2193918/range2193918/1
type: Range
persistent identity: range2193918
display id: range2193918
version: 1
top level: psb1C3_Int
direction: direction/reverse
start: 624
end: 1304
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2193926
display id: annotation2193926
version: 1
top level: psb1C3_Int
location: https://synbiohub.org/public/igem/BBa_K743005/annotation2193926/range2193926/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K743005/annotation2193926/range2193926/1
type: Range
persistent identity: range2193926
display id: range2193926
version: 1
top level: psb1C3_Int
direction: direction/reverse
start: 1338
end: 1466
orientation: inline

BBa_K292003
type: SequenceAnnotation
persistent identity: annotation2193935
display id: annotation2193935
version: 1
top level: psb1C3_Int
location: https://synbiohub.org/public/igem/BBa_K743005/annotation2193935/range2193935/1
component: BBa_K292003

https://synbiohub.org/public/igem/BBa_K743005/annotation2193935/range2193935/1
type: Range
persistent identity: range2193935
display id: range2193935
version: 1
top level: psb1C3_Int
direction: direction/reverse
start: 1475
end: 2544
orientation: inline

BBa_K743001
type: SequenceAnnotation
persistent identity: annotation2193937
display id: annotation2193937
version: 1
top level: psb1C3_Int
location: https://synbiohub.org/public/igem/BBa_K743005/annotation2193937/range2193937/1
component: BBa_K743001

https://synbiohub.org/public/igem/BBa_K743005/annotation2193937/range2193937/1
type: Range
persistent identity: range2193937
display id: range2193937
version: 1
top level: psb1C3_Int
direction: direction/reverse
start: 2553
end: 3239
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

RS1
type: ComponentDefinition
persistent identity: BBa K743000
display id: BBa_K743000
version: 1
was generated by: iGEM to SBOL conversion
description: Synechocystis PCC6803 neutral recombination site.
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:09Z
mutable provenance: PCR amplified from synechocystis PCC6803 chromosome.
owned by: james
owned by: myers
mutable description: Any DNA sequence flacked by this part and BBa_K743001 will be incorporated into Synechocistys PCC6803 chromosome through a neutral double homologous recombination.
discontinued: false
dominant: false
experience: experience/Works
group u list: _994_
m user id: 0
owner id: 11584
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: According to literature, in Synechocystis the recombination sites must be at least 450bp long to allow a rasonably high transformation efficiency, being 700bp the optimum size.
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RS1 recombination site
sequence: https://synbiohub.org/public/igem/BBa_K743000_sequence/1

RS1 recombination site
type: SequenceAnnotation
persistent identity: annotation2188518
display id: annotation2188518
version: 1
top level: RS1
location: https://synbiohub.org/public/igem/BBa_K743000/annotation2188518/range2188518/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K743000/annotation2188518/range2188518/1
type: Range
persistent identity: range2188518
display id: range2188518
version: 1
top level: RS1
direction: direction/forward
start: 1
end: 522
orientation: inline

RS2
type: ComponentDefinition
persistent identity: BBa K743001
display id: BBa_K743001
version: 1
was generated by: iGEM to SBOL conversion
description: Synechocystis PCC6803 neutral recombination site.
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:09Z
mutable provenance: PCR amplified from Synechocystis PCC6803 chromosome, orf slr0337
owned by: james
owned by: myers
mutable description: When placed between this part and BBa_K743000, any DNA sequence will be inserted into Synechocystis PCC6803 chromosome trough homologous recombination
discontinued: false
dominant: false
experience: experience/None
group u list: _994_
m user id: 0
owner id: 11584
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size.
star: false
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: RS2
sequence: https://synbiohub.org/public/igem/BBa_K743001_sequence/1

RS2
type: SequenceAnnotation
persistent identity: annotation2188524
display id: annotation2188524
version: 1
top level: RS2
location: https://synbiohub.org/public/igem/BBa_K743001/annotation2188524/range2188524/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K743001/annotation2188524/range2188524/1
type: Range
persistent identity: range2188524
display id: range2188524
version: 1
top level: RS2
direction: direction/forward
start: 1
end: 687
orientation: inline

BBa_K292003
type: ComponentDefinition
persistent identity: BBa K292003
display id: BBa_K292003
version: 1
was generated by: iGEM to SBOL conversion
description: Kanamycin resistance cassette and a double terminator
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:49Z
mutable provenance: Part:BBa_P1003, Designed by Reshma Shetty Group: Knight Lab, MIT (2006-01-23) Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16)
owned by: james
owned by: myers
mutable description: This part contains a kanamycin resistance cassette and a double terminator (BBa_P1003 + BBa_B0014). This allows the kanamycin cassette to be functional. The cassette can be used to design a recombinant vector by gene insertion in a non lytic bacteriophage as the Lambda phage. In fact it allows us to know if a bacteriophage is modified or not and if the inserted gene is expressed or not. When the virus is modified the kanamycin cassette induces a kanamycin resistance, so the bacteria become resistance, and when the virus is not modified bacteria are killed by kanamycin. This part is completed by B0014 + P1003 + B0014 to finish the transcription before the resistance cassette.
discontinued: false
dominant: false
group u list: _394_
m user id: 0
owner id: 5212
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: When we design the bio-brick it was impossible to obtain a functional bio-brick by using the plasmid SB1AK3. But it's worked with a pSB1A1.
star: false
creator: David Charoy
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0011
component: BBa_B0012
component: BBa_P1003
sequence annotation: BBa_P1003
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_K292003_sequence/1

BBa_B0011
type: Component
persistent identity: component2054562
display id: component2054562
version: 1
top level: BBa_K292003
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component2054558
display id: component2054558
version: 1
top level: BBa_K292003
definition: BBa_B0012
access: public

BBa_P1003
type: Component
persistent identity: component2054557
display id: component2054557
version: 1
top level: BBa_K292003
definition: kanR
access: public

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2054557
display id: annotation2054557
version: 1
top level: BBa_K292003
location: https://synbiohub.org/public/igem/BBa_K292003/annotation2054557/range2054557/1
component: BBa_P1003

https://synbiohub.org/public/igem/BBa_K292003/annotation2054557/range2054557/1
type: Range
persistent identity: range2054557
display id: range2054557
version: 1
top level: BBa_K292003
direction: direction/reverse
start: 1
end: 967
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2054558
display id: annotation2054558
version: 1
top level: BBa_K292003
location: https://synbiohub.org/public/igem/BBa_K292003/annotation2054558/range2054558/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K292003/annotation2054558/range2054558/1
type: Range
persistent identity: range2054558
display id: range2054558
version: 1
top level: BBa_K292003
direction: direction/forward
start: 976
end: 1016
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2054562
display id: annotation2054562
version: 1
top level: BBa_K292003
location: https://synbiohub.org/public/igem/BBa_K292003/annotation2054562/range2054562/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K292003/annotation2054562/range2054562/1
type: Range
persistent identity: range2054562
display id: range2054562
version: 1
top level: BBa_K292003
direction: direction/forward
start: 1025
end: 1070
orientation: inline

BBa_K390017
type: ComponentDefinition
persistent identity: BBa K390017
display id: BBa_K390017
version: 1
was generated by: iGEM to SBOL conversion
description: psbA2 promoter, 5'UTR, and RBS
created: 2010-08-01T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: (coming soon)
owned by: james
owned by: myers
mutable description: (coming soon)
discontinued: false
dominant: false
group u list: _518_
m user id: 0
owner id: 6788
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: (coming soon)
star: false
creator: Cody Tramp
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: psbA2 promoter
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: +1 Transcription Start Point
sequence annotation: putative rbs
sequence: https://synbiohub.org/public/igem/BBa_K390017_sequence/1

psbA2 promoter
type: SequenceAnnotation
persistent identity: annotation2076603
display id: annotation2076603
version: 1
top level: BBa_K390017
location: https://synbiohub.org/public/igem/BBa_K390017/annotation2076603/range2076603/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K390017/annotation2076603/range2076603/1
type: Range
persistent identity: range2076603
display id: range2076603
version: 1
top level: BBa_K390017
direction: direction/forward
start: 1
end: 38
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2076605
display id: annotation2076605
version: 1
top level: BBa_K390017
location: https://synbiohub.org/public/igem/BBa_K390017/annotation2076605/range2076605/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K390017/annotation2076605/range2076605/1
type: Range
persistent identity: range2076605
display id: range2076605
version: 1
top level: BBa_K390017
direction: direction/forward
start: 3
end: 8
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2076606
display id: annotation2076606
version: 1
top level: BBa_K390017
location: https://synbiohub.org/public/igem/BBa_K390017/annotation2076606/range2076606/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K390017/annotation2076606/range2076606/1
type: Range
persistent identity: range2076606
display id: range2076606
version: 1
top level: BBa_K390017
direction: direction/forward
start: 27
end: 32
orientation: inline

+1 Transcription Start Point
type: SequenceAnnotation
persistent identity: annotation2076607
display id: annotation2076607
version: 1
top level: BBa_K390017
location: https://synbiohub.org/public/igem/BBa_K390017/annotation2076607/range2076607/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K390017/annotation2076607/range2076607/1
type: Range
persistent identity: range2076607
display id: range2076607
version: 1
top level: BBa_K390017
direction: direction/forward
start: 39
end: 39
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2076604
display id: annotation2076604
version: 1
top level: BBa_K390017
location: https://synbiohub.org/public/igem/BBa_K390017/annotation2076604/range2076604/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K390017/annotation2076604/range2076604/1
type: Range
persistent identity: range2076604
display id: range2076604
version: 1
top level: BBa_K390017
direction: direction/forward
start: 71
end: 80
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

kanR
type: ComponentDefinition
persistent identity: BBa P1003
display id: BBa_P1003
version: 1
was generated by: iGEM to SBOL conversion
description: kanamycin resistance cassette
created: 2006-01-23T12:00:00Z
modified: 2015-05-08T01:14:11Z
owned by: james
owned by: myers
mutable description: Kanamycin resistance cassette for modular BioBricks vector construction.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: kanamycin resistance
sequence: https://synbiohub.org/public/igem/BBa_P1003_sequence/1

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation1897112
display id: annotation1897112
version: 1
top level: kanR
location: https://synbiohub.org/public/igem/BBa_P1003/annotation1897112/range1897112/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_P1003/annotation1897112/range1897112/1
type: Range
persistent identity: range1897112
display id: range1897112
version: 1
top level: kanR
direction: direction/forward
start: 149
end: 967
orientation: inline

https://synbiohub.org/public/igem/BBa_K743005_sequence/1
type: Sequence
persistent identity: BBa K743005 sequence
display id: BBa_K743005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccagcctctcaaccaccgatattgggcccctagctacccctgatattttggcggacattgttgcgcaagtagaacaaaccattgctgctggagcccactgtcgttgtggcggccaagccctagaccaaccgggaaattactatcctcccaccctgctcaccgacgttccccccaacgcccctacctaccgtcaggaattttttggccccgtggccctcggatttactgtcgataatttagaggaggcgatcgccttggccaatgacattccctttgggttgggggccagtgcatggacaactaacccggaaaatcaacaaaaactaatccggggcatagaagccggggctgtattcattaacggtatgactaaatctgacccccgcattccctttggaggcatcaagcgctctggctttgggcgggaacttggccgcatgggcattttagaatttgtcaatgctaaaaccgtttggattgcttagtccgccaccattttaaaattatcgctcttctattctactagaggctttacaaaactctcattaatcctttagactaagtttagtcagttccaatctgaacatcgacaaatacataaggaattataaccaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K743000_sequence/1
type: Sequence
persistent identity: BBa K743000 sequence
display id: BBa_K743000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccagcctctcaaccaccgatattgggcccctagctacccctgatattttggcggacattgttgcgcaagtagaacaaaccattgctgctggagcccactgtcgttgtggcggccaagccctagaccaaccgggaaattactatcctcccaccctgctcaccgacgttccccccaacgcccctacctaccgtcaggaattttttggccccgtggccctcggatttactgtcgataatttagaggaggcgatcgccttggccaatgacattccctttgggttgggggccagtgcatggacaactaacccggaaaatcaacaaaaactaatccggggcatagaagccggggctgtattcattaacggtatgactaaatctgacccccgcattccctttggaggcatcaagcgctctggctttgggcgggaacttggccgcatgggcattttagaatttgtcaatgctaaaaccgtttggattgcttagtccgccaccattttaaaattatcgctcttctattc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K743001_sequence/1
type: Sequence
persistent identity: BBa K743001 sequence
display id: BBa_K743001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtgtaggcggtaaagtctatgggaacttccagtttaagggctgtcccgttaaatatggcttcgagtttttgaagcataattttctaacgtctgtggatttttttaagcatagcttgaccttctgttggtcgaattctagggattttcatccatattcggttcatagggcagctcaatggtgaacattgctcccccagtttcatggttagctgcggttaaatttcccctgtgggccaggacaatttccttggcgatcgccaaacccaggccactgccatggcgttgactatcgggatgtaggcgagcacgggaactatcaccccgataaagtcgctcaaaaatgtagggtaaatcccttggttgaaatcctaggccttgatccctaataatcatggtgatcccttctgtgccttgatgaccctgcacaaaaatagtcccttccggtgggctatatttcagcgtattgtccaaaatattcatcaatacctgcatcagacgatcgccatccccctctaggttcaacttggttggcccttggtaatccagggtgatattttttaccacggcgatcggggttaatcgctcccaggtaatggcgagtaaatggcgtaaattaatcggttctggctcgaggtaaagattgggattagcggttatttgggttaaatggagccaactttccacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K292003_sequence/1
type: Sequence
persistent identity: BBa K292003 sequence
display id: BBa_K292003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K390017_sequence/1
type: Sequence
persistent identity: BBa K390017 sequence
display id: BBa_K390017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctttacaaaactctcattaatcctttagactaagtttagtcagttccaatctgaacatcgacaaatacataaggaattataaccaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1003_sequence/1
type: Sequence
persistent identity: BBa P1003 sequence
display id: BBa_P1003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z