psb1C3_IntK recombination plasmid for Synechocystis PCC6803
RS1 and RS2 were obtained from Synechocystis genomic DNA, both the kanamycin resistance (BBa_P1003) and the double terminator (BBa_B0015) are from the registry of standard biological parts.
This plasmid allows the integration of a Kanamycin resistance casette followed by a double terminator in to Synechocystis chromosome, this allows for selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coli The use of BBa_K743000 and BBa_K743001 as recombination sites has no phenotypic effects on the cells. The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly. It was used by UC_Chile 2012 team to succesfully transform Synechocystis [http://2012.igem.org/Team:UC_Chile]
Double terminator consisting of BBa_B0010 and BBa_B0012
Kanamycin resistance cassette for modular BioBricks vector construction.
Synechocystis PCC6803 neutral recombination site.
PCR amplified from synechocystis PCC6803 chromosome.
Any DNA sequence flacked by this part and BBa_K743001 will be incorporated into Synechocistys PCC6803 chromosome through a neutral double homologous recombination.
According to literature, in Synechocystis the recombination sites must be at least 450bp long to allow a rasonably high transformation efficiency, being 700bp the optimum size.
PCR amplified from Synechocystis PCC6803 chromosome, orf slr0337
When placed between this part and BBa_K743000, any DNA sequence will be inserted into Synechocystis PCC6803 chromosome trough homologous recombination
According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1