psb1C3_int
type: ComponentDefinition
persistent identity: BBa K743006
display id: BBa_K743006
version: 1
was generated by: iGEM to SBOL conversion
description: psb1C3_IntK recombination plasmid for Synechocystis PCC6803
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:09Z
mutable provenance: RS1 and RS2 were obtained from Synechocystis genomic DNA, both the kanamycin resistance (BBa_P1003) and the double terminator (BBa_B0015) are from the registry of standard biological parts.
owned by: james
owned by: myers
mutable description: This plasmid allows the integration of a Kanamycin resistance casette followed by a double terminator in to Synechocystis chromosome, this allows for selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coli The use of BBa_K743000 and BBa_K743001 as recombination sites has no phenotypic effects on the cells. The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly. It was used by UC_Chile 2012 team to succesfully transform Synechocystis [http://2012.igem.org/Team:UC_Chile]
discontinued: false
dominant: false
experience: experience/Works
group u list: _994_
m user id: 0
owner id: 11584
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_S05069
component: BBa_S05070
sequence annotation: BBa_S05070
sequence annotation: BBa_S05069
sequence: https://synbiohub.org/public/igem/BBa_K743006_sequence/1

BBa_S05069
type: Component
persistent identity: component2188788
display id: component2188788
version: 1
top level: psb1C3_int
definition: BBa_S05069
access: public

BBa_S05070
type: Component
persistent identity: component2188778
display id: component2188778
version: 1
top level: psb1C3_int
definition: BBa_S05070
access: public

BBa_S05070
type: SequenceAnnotation
persistent identity: annotation2188778
display id: annotation2188778
version: 1
top level: psb1C3_int
location: https://synbiohub.org/public/igem/BBa_K743006/annotation2188778/range2188778/1
component: BBa_S05070

https://synbiohub.org/public/igem/BBa_K743006/annotation2188778/range2188778/1
type: Range
persistent identity: range2188778
display id: range2188778
version: 1
top level: psb1C3_int
direction: direction/reverse
start: 1
end: 1497
orientation: inline

BBa_S05069
type: SequenceAnnotation
persistent identity: annotation2188788
display id: annotation2188788
version: 1
top level: psb1C3_int
location: https://synbiohub.org/public/igem/BBa_K743006/annotation2188788/range2188788/1
component: BBa_S05069

https://synbiohub.org/public/igem/BBa_K743006/annotation2188788/range2188788/1
type: Range
persistent identity: range2188788
display id: range2188788
version: 1
top level: psb1C3_int
direction: direction/reverse
start: 1506
end: 2329
orientation: inline

BBa_S05069
type: ComponentDefinition
persistent identity: BBa S05069
display id: BBa_S05069
version: 1
was generated by: iGEM to SBOL conversion
description: B0015:K743001
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:14:49Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/Works
group u list: _9_
m user id: 0
owner id: 11584
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K743001
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_K743001
sequence: https://synbiohub.org/public/igem/BBa_S05069_sequence/1

BBa_K743001
type: Component
persistent identity: component2188700
display id: component2188700
version: 1
top level: BBa_S05069
definition: RS2
access: public

BBa_B0015
type: Component
persistent identity: component2188698
display id: component2188698
version: 1
top level: BBa_S05069
definition: BBa_B0015
access: public

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2188698
display id: annotation2188698
version: 1
top level: BBa_S05069
location: https://synbiohub.org/public/igem/BBa_S05069/annotation2188698/range2188698/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_S05069/annotation2188698/range2188698/1
type: Range
persistent identity: range2188698
display id: range2188698
version: 1
top level: BBa_S05069
direction: direction/reverse
start: 1
end: 129
orientation: inline

BBa_K743001
type: SequenceAnnotation
persistent identity: annotation2188700
display id: annotation2188700
version: 1
top level: BBa_S05069
location: https://synbiohub.org/public/igem/BBa_S05069/annotation2188700/range2188700/1
component: BBa_K743001

https://synbiohub.org/public/igem/BBa_S05069/annotation2188700/range2188700/1
type: Range
persistent identity: range2188700
display id: range2188700
version: 1
top level: BBa_S05069
direction: direction/reverse
start: 138
end: 824
orientation: inline

BBa_S05070
type: ComponentDefinition
persistent identity: BBa S05070
display id: BBa_S05070
version: 1
was generated by: iGEM to SBOL conversion
description: K743000:P1003
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:14:49Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 11584
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Juan Sim??n ??lamos
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_P1003
component: BBa_K743000
sequence annotation: BBa_K743000
sequence annotation: BBa_P1003
sequence: https://synbiohub.org/public/igem/BBa_S05070_sequence/1

BBa_P1003
type: Component
persistent identity: component2188704
display id: component2188704
version: 1
top level: BBa_S05070
definition: kanR
access: public

BBa_K743000
type: Component
persistent identity: component2188702
display id: component2188702
version: 1
top level: BBa_S05070
definition: RS1
access: public

BBa_K743000
type: SequenceAnnotation
persistent identity: annotation2188702
display id: annotation2188702
version: 1
top level: BBa_S05070
location: https://synbiohub.org/public/igem/BBa_S05070/annotation2188702/range2188702/1
component: BBa_K743000

https://synbiohub.org/public/igem/BBa_S05070/annotation2188702/range2188702/1
type: Range
persistent identity: range2188702
display id: range2188702
version: 1
top level: BBa_S05070
direction: direction/reverse
start: 1
end: 522
orientation: inline

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2188704
display id: annotation2188704
version: 1
top level: BBa_S05070
location: https://synbiohub.org/public/igem/BBa_S05070/annotation2188704/range2188704/1
component: BBa_P1003

https://synbiohub.org/public/igem/BBa_S05070/annotation2188704/range2188704/1
type: Range
persistent identity: range2188704
display id: range2188704
version: 1
top level: BBa_S05070
direction: direction/reverse
start: 531
end: 1497
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

kanR
type: ComponentDefinition
persistent identity: BBa P1003
display id: BBa_P1003
version: 1
was generated by: iGEM to SBOL conversion
description: kanamycin resistance cassette
created: 2006-01-23T12:00:00Z
modified: 2015-05-08T01:14:11Z
owned by: james
owned by: myers
mutable description: Kanamycin resistance cassette for modular BioBricks vector construction.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: kanamycin resistance
sequence: https://synbiohub.org/public/igem/BBa_P1003_sequence/1

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation1897112
display id: annotation1897112
version: 1
top level: kanR
location: https://synbiohub.org/public/igem/BBa_P1003/annotation1897112/range1897112/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_P1003/annotation1897112/range1897112/1
type: Range
persistent identity: range1897112
display id: range1897112
version: 1
top level: kanR
direction: direction/forward
start: 149
end: 967
orientation: inline

RS1
type: ComponentDefinition
persistent identity: BBa K743000
display id: BBa_K743000
version: 1
was generated by: iGEM to SBOL conversion
description: Synechocystis PCC6803 neutral recombination site.
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:09Z
mutable provenance: PCR amplified from synechocystis PCC6803 chromosome.
owned by: james
owned by: myers
mutable description: Any DNA sequence flacked by this part and BBa_K743001 will be incorporated into Synechocistys PCC6803 chromosome through a neutral double homologous recombination.
discontinued: false
dominant: false
experience: experience/Works
group u list: _994_
m user id: 0
owner id: 11584
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: According to literature, in Synechocystis the recombination sites must be at least 450bp long to allow a rasonably high transformation efficiency, being 700bp the optimum size.
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RS1 recombination site
sequence: https://synbiohub.org/public/igem/BBa_K743000_sequence/1

RS1 recombination site
type: SequenceAnnotation
persistent identity: annotation2188518
display id: annotation2188518
version: 1
top level: RS1
location: https://synbiohub.org/public/igem/BBa_K743000/annotation2188518/range2188518/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K743000/annotation2188518/range2188518/1
type: Range
persistent identity: range2188518
display id: range2188518
version: 1
top level: RS1
direction: direction/forward
start: 1
end: 522
orientation: inline

RS2
type: ComponentDefinition
persistent identity: BBa K743001
display id: BBa_K743001
version: 1
was generated by: iGEM to SBOL conversion
description: Synechocystis PCC6803 neutral recombination site.
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:09Z
mutable provenance: PCR amplified from Synechocystis PCC6803 chromosome, orf slr0337
owned by: james
owned by: myers
mutable description: When placed between this part and BBa_K743000, any DNA sequence will be inserted into Synechocystis PCC6803 chromosome trough homologous recombination
discontinued: false
dominant: false
experience: experience/None
group u list: _994_
m user id: 0
owner id: 11584
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size.
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RS2
sequence: https://synbiohub.org/public/igem/BBa_K743001_sequence/1

RS2
type: SequenceAnnotation
persistent identity: annotation2188524
display id: annotation2188524
version: 1
top level: RS2
location: https://synbiohub.org/public/igem/BBa_K743001/annotation2188524/range2188524/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K743001/annotation2188524/range2188524/1
type: Range
persistent identity: range2188524
display id: range2188524
version: 1
top level: RS2
direction: direction/forward
start: 1
end: 687
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K743006_sequence/1
type: Sequence
persistent identity: BBa K743006 sequence
display id: BBa_K743006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccagcctctcaaccaccgatattgggcccctagctacccctgatattttggcggacattgttgcgcaagtagaacaaaccattgctgctggagcccactgtcgttgtggcggccaagccctagaccaaccgggaaattactatcctcccaccctgctcaccgacgttccccccaacgcccctacctaccgtcaggaattttttggccccgtggccctcggatttactgtcgataatttagaggaggcgatcgccttggccaatgacattccctttgggttgggggccagtgcatggacaactaacccggaaaatcaacaaaaactaatccggggcatagaagccggggctgtattcattaacggtatgactaaatctgacccccgcattccctttggaggcatcaagcgctctggctttgggcgggaacttggccgcatgggcattttagaatttgtcaatgctaaaaccgtttggattgcttagtccgccaccattttaaaattatcgctcttctattctactagagctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagtgtaggcggtaaagtctatgggaacttccagtttaagggctgtcccgttaaatatggcttcgagtttttgaagcataattttctaacgtctgtggatttttttaagcatagcttgaccttctgttggtcgaattctagggattttcatccatattcggttcatagggcagctcaatggtgaacattgctcccccagtttcatggttagctgcggttaaatttcccctgtgggccaggacaatttccttggcgatcgccaaacccaggccactgccatggcgttgactatcgggatgtaggcgagcacgggaactatcaccccgataaagtcgctcaaaaatgtagggtaaatccct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05069_sequence/1
type: Sequence
persistent identity: BBa S05069 sequence
display id: BBa_S05069_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagtgtaggcggtaaagtctatgggaacttccagtttaagggctgtcccgttaaatatggcttcgagtttttgaagcataattttctaacgtctgtggatttttttaagcatagcttgaccttctgttggtcgaattctagggattttcatccatattcggttcatagggcagctcaatggtgaacattgctcccccagtttcatggttagctgcggttaaatttcccctgtgggccaggacaatttccttggcgatcgccaaacccaggccactgccatggcgttgactatcgggatgtaggcgagcacgggaactatcaccccgataaagtcgctcaaaaatgtagggtaaatcccttggttgaaatcctaggccttgatccctaataatcatggtgatcccttctgtgccttgatgaccctgcacaaaaatagtcccttccggtgggctatatttcagcgtattgtccaaaatattcatcaatacctgcatcagacgatcgccatccccctctaggttcaacttggttggcccttggtaatccagggtgatattttttaccacggcgatcggggttaatcgctcccaggtaatggcgagtaaatggcgtaaattaatcggttctggctcgaggtaaagattgggattagcggttatttgggttaaatggagccaactttccacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05070_sequence/1
type: Sequence
persistent identity: BBa S05070 sequence
display id: BBa_S05070_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccagcctctcaaccaccgatattgggcccctagctacccctgatattttggcggacattgttgcgcaagtagaacaaaccattgctgctggagcccactgtcgttgtggcggccaagccctagaccaaccgggaaattactatcctcccaccctgctcaccgacgttccccccaacgcccctacctaccgtcaggaattttttggccccgtggccctcggatttactgtcgataatttagaggaggcgatcgccttggccaatgacattccctttgggttgggggccagtgcatggacaactaacccggaaaatcaacaaaaactaatccggggcatagaagccggggctgtattcattaacggtatgactaaatctgacccccgcattccctttggaggcatcaagcgctctggctttgggcgggaacttggccgcatgggcattttagaatttgtcaatgctaaaaccgtttggattgcttagtccgccaccattttaaaattatcgctcttctattctactagagctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1003_sequence/1
type: Sequence
persistent identity: BBa P1003 sequence
display id: BBa_P1003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K743000_sequence/1
type: Sequence
persistent identity: BBa K743000 sequence
display id: BBa_K743000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccagcctctcaaccaccgatattgggcccctagctacccctgatattttggcggacattgttgcgcaagtagaacaaaccattgctgctggagcccactgtcgttgtggcggccaagccctagaccaaccgggaaattactatcctcccaccctgctcaccgacgttccccccaacgcccctacctaccgtcaggaattttttggccccgtggccctcggatttactgtcgataatttagaggaggcgatcgccttggccaatgacattccctttgggttgggggccagtgcatggacaactaacccggaaaatcaacaaaaactaatccggggcatagaagccggggctgtattcattaacggtatgactaaatctgacccccgcattccctttggaggcatcaagcgctctggctttgggcgggaacttggccgcatgggcattttagaatttgtcaatgctaaaaccgtttggattgcttagtccgccaccattttaaaattatcgctcttctattc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K743001_sequence/1
type: Sequence
persistent identity: BBa K743001 sequence
display id: BBa_K743001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtgtaggcggtaaagtctatgggaacttccagtttaagggctgtcccgttaaatatggcttcgagtttttgaagcataattttctaacgtctgtggatttttttaagcatagcttgaccttctgttggtcgaattctagggattttcatccatattcggttcatagggcagctcaatggtgaacattgctcccccagtttcatggttagctgcggttaaatttcccctgtgggccaggacaatttccttggcgatcgccaaacccaggccactgccatggcgttgactatcgggatgtaggcgagcacgggaactatcaccccgataaagtcgctcaaaaatgtagggtaaatcccttggttgaaatcctaggccttgatccctaataatcatggtgatcccttctgtgccttgatgaccctgcacaaaaatagtcccttccggtgggctatatttcagcgtattgtccaaaatattcatcaatacctgcatcagacgatcgccatccccctctaggttcaacttggttggcccttggtaatccagggtgatattttttaccacggcgatcggggttaatcgctcccaggtaatggcgagtaaatggcgtaaattaatcggttctggctcgaggtaaagattgggattagcggttatttgggttaaatggagccaactttccacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z