LOCUS       BBa_K743007             1634 bp    DNA     linear       28-Sep-2024
DEFINITION  psb1C3_IntKR recombination plasmid for Synechocystis PCC6803
ACCESSION   BBa_K743007
VERSION     BBa_K743007.1  
FEATURES             Location/Qualifiers
     misc_feature    1..522
                     /label=RS1 recombination site
     misc_feature    1..522
                     /label=BBa_K743000
     stem_loop       542..585
                     /label=stem_loop
     misc_feature    531..610
                     /label=BBa_B0010
     terminator      531..610
                     /label=BBa_B0010
     stem_loop       626..645
                     /label=T7 TE
     misc_feature    652..652
                     /label=stop
     polyA_site      646..659
                     /label=polya
     misc_feature    619..659
                     /label=BBa_B0012
     terminator      619..659
                     /label=BBa_B0012
     terminator      531..659
                     /label=BBa_B0015
     CDS             668..1486
                     /label=kanamycin resistance
     misc_feature    1454..1454
                     /label=G to C mutation to eliminate XhoI site
     misc_feature    1591..1634
                     /label=repeat region
     misc_feature    668..1634
                     /label=BBa_P1007
     misc_feature    531..1634
                     /label=BBa_S05073
ORIGIN
        1 tcccagcctc tcaaccaccg atattgggcc cctagctacc cctgatattt tggcggacat
       61 tgttgcgcaa gtagaacaaa ccattgctgc tggagcccac tgtcgttgtg gcggccaagc
      121 cctagaccaa ccgggaaatt actatcctcc caccctgctc accgacgttc cccccaacgc
      181 ccctacctac cgtcaggaat tttttggccc cgtggccctc ggatttactg tcgataattt
      241 agaggaggcg atcgccttgg ccaatgacat tccctttggg ttgggggcca gtgcatggac
      301 aactaacccg gaaaatcaac aaaaactaat ccggggcata gaagccgggg ctgtattcat
      361 taacggtatg actaaatctg acccccgcat tccctttgga ggcatcaagc gctctggctt
      421 tgggcgggaa cttggccgca tgggcatttt agaatttgtc aatgctaaaa ccgtttggat
      481 tgcttagtcc gccaccattt taaaattatc gctcttctat tctactagag ccaggcatca
      541 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      601 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
      661 actagagtta ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
      721 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
      781 ggcagttcca aagaatggca aggtcctggt aacggtctgc gattccgacc cgtccaacat
      841 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
      901 gagtgacgac tgaatccggt gagaatggca agagcttgtg catttctttc cagacttgtt
      961 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca
     1021 tgcgtgattg cgcctgagca agacgaaata cacgatcgct gttaaaagga caattacaaa
     1081 caggaatcga atgtaaccgg cgcaggaaca cggccagcgc atcaacaata ttttcacctg
     1141 aatcaggata ttcttctaat acctggaagg ctgttttccc aggaatcgcg gtggtgagta
     1201 accacgcatc atcaggagta cggataaaat gcttgatggt cgggagaggc ataaactccg
     1261 tcagccagtt gagacggacc atctcatctg taacatcatt ggcaacgcta cctttgccat
     1321 gtttcagaaa caactctggc gcatcgggct tcccatacaa gcgatagatt gtcgcacctg
     1381 attgcccgac attatcgcga gcccatttat acccatataa atcagcgtcc atgttggagt
     1441 ttaagcgcgg acgggagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat
     1501 tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa
     1561 tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga acttttgctg
     1621 agttgaagga tcag
//