##gff-version 3
##sequence-region BBa_K743008 1 2313
BBa_K743008	.	sequence_feature	1	522	.	+	0	ID=BBa_K743000;Name=BBa_K743000
BBa_K743008	.	sequence_feature	1	522	.	+	0	ID=annotation2188518;Name=RS1 recombination site;Parent=BBa_K743000
BBa_K743008	.	engineered_region	523	1626	.	+	0	ID=BBa_S05073;Name=BBa_S05073
BBa_K743008	.	engineered_region	660	1626	.	+	0	ID=BBa_P1007;Name=BBa_P1007;Parent=BBa_S05073
BBa_K743008	.	point_mutation	1446	1446	.	+	0	ID=annotation1919400;Name=G to C mutation to eliminate XhoI site;Parent=BBa_P1007
BBa_K743008	.	CDS	660	1478	.	+	0	ID=annotation1919399;Name=kanamycin resistance;Parent=BBa_P1007
BBa_K743008	.	sequence_feature	1583	1626	.	+	0	ID=annotation1919401;Name=repeat region;Parent=BBa_P1007
BBa_K743008	.	terminator	523	651	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S05073
BBa_K743008	.	terminator	523	602	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K743008	.	engineered_region	523	602	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K743008	.	stem_loop	534	577	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K743008	.	terminator	611	651	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K743008	.	stop_codon	644	644	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K743008	.	polyA_site	638	651	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K743008	.	stem_loop	618	637	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K743008	.	engineered_region	611	651	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K743008	.	sequence_feature	1627	2313	.	+	0	ID=BBa_K743001;Name=BBa_K743001
BBa_K743008	.	sequence_feature	1627	2313	.	+	0	ID=annotation2188524;Name=RS2;Parent=BBa_K743001
>BBa_K743008
tcccagcctctcaaccaccgatattgggcccctagctacccctgatattttggcggacattgttgcgcaagtagaacaa
accattgctgctggagcccactgtcgttgtggcggccaagccctagaccaaccgggaaattactatcctcccaccctgc
tcaccgacgttccccccaacgcccctacctaccgtcaggaattttttggccccgtggccctcggatttactgtcgataa
tttagaggaggcgatcgccttggccaatgacattccctttgggttgggggccagtgcatggacaactaacccggaaaat
caacaaaaactaatccggggcatagaagccggggctgtattcattaacggtatgactaaatctgacccccgcattccct
ttggaggcatcaagcgctctggctttgggcgggaacttggccgcatgggcattttagaatttgtcaatgctaaaaccgt
ttggattgcttagtccgccaccattttaaaattatcgctcttctattcccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagttattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatca
ggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccaaagaatgg
caaggtcctggtaacggtctgcgattccgacccgtccaacatcaatacaacctattaatttcccctcgtcaaaaataag
gttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaagagcttgtgcatttctttccagact
tgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcatgcgtgattgcgcct
gagcaagacgaaatacacgatcgctgttaaaaggacaattacaaacaggaatcgaatgtaaccggcgcaggaacacggc
cagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaaggctgttttcccaggaatcgcggtg
gtgagtaaccacgcatcatcaggagtacggataaaatgcttgatggtcgggagaggcataaactccgtcagccagttga
gacggaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggctt
cccatacaagcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcgtcc
atgttggagtttaagcgcggacgggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgttta
tgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaac
gtggctttgttgaataaatcgaacttttgctgagttgaaggatcagagtgtaggcggtaaagtctatgggaacttccag
tttaagggctgtcccgttaaatatggcttcgagtttttgaagcataattttctaacgtctgtggatttttttaagcata
gcttgaccttctgttggtcgaattctagggattttcatccatattcggttcatagggcagctcaatggtgaacattgct
cccccagtttcatggttagctgcggttaaatttcccctgtgggccaggacaatttccttggcgatcgccaaacccaggc
cactgccatggcgttgactatcgggatgtaggcgagcacgggaactatcaccccgataaagtcgctcaaaaatgtaggg
taaatcccttggttgaaatcctaggccttgatccctaataatcatggtgatcccttctgtgccttgatgaccctgcaca
aaaatagtcccttccggtgggctatatttcagcgtattgtccaaaatattcatcaatacctgcatcagacgatcgccat
ccccctctaggttcaacttggttggcccttggtaatccagggtgatattttttaccacggcgatcggggttaatcgctc
ccaggtaatggcgagtaaatggcgtaaattaatcggttctggctcgaggtaaagattgggattagcggttatttgggtt
aaatggagccaactttccacca