LOCUS       BBa_K743009             3212 bp    DNA     linear       17-May-2024
DEFINITION  psb1C3_IntKR recombination plasmid with RFP under psaAB promoter
ACCESSION   BBa_K743009
VERSION     BBa_K743009.1  
FEATURES             Location/Qualifiers
     misc_feature    1..522
                     /label=RS1 recombination site
     misc_feature    1..522
                     /label=BBa_K743000
     promoter        531..569
                     /label=psaAB operon promoter
     misc_feature    570..572
                     /label=+1 Transcription Start Point(s)
     misc_feature    699..707
                     /label=rbs region (non exact)
     misc_feature    535..540
                     /label=-35 box
     misc_feature    557..562
                     /label=-10 box
     promoter        523..715
                     /label=BBa_K390016
     CDS             716..1390
                     /label=mrfp1
     misc_feature    1397..1421
                     /label=Help:Barcodes
     CDS             716..1421
                     /label=BBa_E1010
     misc_feature    523..1421
                     /label=BBa_S05075
     stem_loop       1433..1476
                     /label=stem_loop
     misc_feature    1422..1501
                     /label=BBa_B0010
     terminator      1422..1501
                     /label=BBa_B0010
     stem_loop       1517..1536
                     /label=T7 TE
     misc_feature    1543..1543
                     /label=stop
     polyA_site      1537..1550
                     /label=polya
     misc_feature    1510..1550
                     /label=BBa_B0012
     terminator      1510..1550
                     /label=BBa_B0012
     terminator      1422..1550
                     /label=BBa_B0015
     CDS             1559..2377
                     /label=kanamycin resistance
     misc_feature    2345..2345
                     /label=G to C mutation to eliminate XhoI site
     misc_feature    2482..2525
                     /label=repeat region
     misc_feature    1559..2525
                     /label=BBa_P1007
     misc_feature    1422..2525
                     /label=BBa_S05073
     misc_feature    2526..3212
                     /label=RS2
     misc_feature    2526..3212
                     /label=BBa_K743001
ORIGIN
        1 tcccagcctc tcaaccaccg atattgggcc cctagctacc cctgatattt tggcggacat
       61 tgttgcgcaa gtagaacaaa ccattgctgc tggagcccac tgtcgttgtg gcggccaagc
      121 cctagaccaa ccgggaaatt actatcctcc caccctgctc accgacgttc cccccaacgc
      181 ccctacctac cgtcaggaat tttttggccc cgtggccctc ggatttactg tcgataattt
      241 agaggaggcg atcgccttgg ccaatgacat tccctttggg ttgggggcca gtgcatggac
      301 aactaacccg gaaaatcaac aaaaactaat ccggggcata gaagccgggg ctgtattcat
      361 taacggtatg actaaatctg acccccgcat tccctttgga ggcatcaagc gctctggctt
      421 tgggcgggaa cttggccgca tgggcatttt agaatttgtc aatgctaaaa ccgtttggat
      481 tgcttagtcc gccaccattt taaaattatc gctcttctat tcgttacgca ggtcttgcct
      541 aggggggggg aggccgtatt atcttctagt gatgtttgct gaaaacgcct atctgtgcaa
      601 ggtttaacat cgttattatg aagcgaaaac taattccctt ttttacgctt cctctattac
      661 actattctgc ataggaaacc cttaatagtt cattgtcgag cgaggagaac cctgcatggc
      721 ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
      781 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
      841 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
      901 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
      961 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg
     1021 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
     1081 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
     1141 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa
     1201 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
     1261 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
     1321 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
     1381 caccggtgct taataacgct gatagtgcta gtgtagatcg cccaggcatc aaataaaacg
     1441 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1501 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtt
     1561 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     1621 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     1681 aaagaatggc aaggtcctgg taacggtctg cgattccgac ccgtccaaca tcaatacaac
     1741 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     1801 ctgaatccgg tgagaatggc aagagcttgt gcatttcttt ccagacttgt tcaacaggcc
     1861 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc atgcgtgatt
     1921 gcgcctgagc aagacgaaat acacgatcgc tgttaaaagg acaattacaa acaggaatcg
     1981 aatgtaaccg gcgcaggaac acggccagcg catcaacaat attttcacct gaatcaggat
     2041 attcttctaa tacctggaag gctgttttcc caggaatcgc ggtggtgagt aaccacgcat
     2101 catcaggagt acggataaaa tgcttgatgg tcgggagagg cataaactcc gtcagccagt
     2161 tgagacggac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     2221 acaactctgg cgcatcgggc ttcccataca agcgatagat tgtcgcacct gattgcccga
     2281 cattatcgcg agcccattta tacccatata aatcagcgtc catgttggag tttaagcgcg
     2341 gacgggagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     2401 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     2461 agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg
     2521 atcagagtgt aggcggtaaa gtctatggga acttccagtt taagggctgt cccgttaaat
     2581 atggcttcga gtttttgaag cataattttc taacgtctgt ggattttttt aagcatagct
     2641 tgaccttctg ttggtcgaat tctagggatt ttcatccata ttcggttcat agggcagctc
     2701 aatggtgaac attgctcccc cagtttcatg gttagctgcg gttaaatttc ccctgtgggc
     2761 caggacaatt tccttggcga tcgccaaacc caggccactg ccatggcgtt gactatcggg
     2821 atgtaggcga gcacgggaac tatcaccccg ataaagtcgc tcaaaaatgt agggtaaatc
     2881 ccttggttga aatcctaggc cttgatccct aataatcatg gtgatccctt ctgtgccttg
     2941 atgaccctgc acaaaaatag tcccttccgg tgggctatat ttcagcgtat tgtccaaaat
     3001 attcatcaat acctgcatca gacgatcgcc atccccctct aggttcaact tggttggccc
     3061 ttggtaatcc agggtgatat tttttaccac ggcgatcggg gttaatcgct cccaggtaat
     3121 ggcgagtaaa tggcgtaaat taatcggttc tggctcgagg taaagattgg gattagcggt
     3181 tatttgggtt aaatggagcc aactttccac ca
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