##gff-version 3
##sequence-region BBa_K743009 1 3212
BBa_K743009	.	engineered_region	523	1421	.	+	0	ID=BBa_S05075;Name=BBa_S05075
BBa_K743009	.	CDS	716	1421	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_S05075
BBa_K743009	.	CDS	716	1390	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K743009	.	engineered_tag	1397	1421	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K743009	.	promoter	523	715	.	+	0	ID=BBa_K390016;Name=BBa_K390016;Parent=BBa_S05075
BBa_K743009	.	sequence_feature	535	540	.	+	0	ID=annotation2076601;Name=-35 box;Parent=BBa_K390016
BBa_K743009	.	sequence_feature	699	707	.	+	0	ID=annotation2076600;Name=rbs region (non exact);Parent=BBa_K390016
BBa_K743009	.	sequence_feature	570	572	.	+	0	ID=annotation2076599;Name=+1 Transcription Start Point(s);Parent=BBa_K390016
BBa_K743009	.	promoter	531	569	.	+	0	ID=annotation2076598;Name=psaAB operon promoter;Parent=BBa_K390016
BBa_K743009	.	sequence_feature	557	562	.	+	0	ID=annotation2076602;Name=-10 box;Parent=BBa_K390016
BBa_K743009	.	sequence_feature	2526	3212	.	+	0	ID=BBa_K743001;Name=BBa_K743001
BBa_K743009	.	sequence_feature	2526	3212	.	+	0	ID=annotation2188524;Name=RS2;Parent=BBa_K743001
BBa_K743009	.	sequence_feature	1	522	.	+	0	ID=BBa_K743000;Name=BBa_K743000
BBa_K743009	.	sequence_feature	1	522	.	+	0	ID=annotation2188518;Name=RS1 recombination site;Parent=BBa_K743000
BBa_K743009	.	engineered_region	1422	2525	.	+	0	ID=BBa_S05073;Name=BBa_S05073
BBa_K743009	.	engineered_region	1559	2525	.	+	0	ID=BBa_P1007;Name=BBa_P1007;Parent=BBa_S05073
BBa_K743009	.	CDS	1559	2377	.	+	0	ID=annotation1919399;Name=kanamycin resistance;Parent=BBa_P1007
BBa_K743009	.	sequence_feature	2482	2525	.	+	0	ID=annotation1919401;Name=repeat region;Parent=BBa_P1007
BBa_K743009	.	point_mutation	2345	2345	.	+	0	ID=annotation1919400;Name=G to C mutation to eliminate XhoI site;Parent=BBa_P1007
BBa_K743009	.	terminator	1422	1550	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S05073
BBa_K743009	.	terminator	1422	1501	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K743009	.	engineered_region	1422	1501	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K743009	.	stem_loop	1433	1476	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K743009	.	terminator	1510	1550	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K743009	.	engineered_region	1510	1550	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K743009	.	polyA_site	1537	1550	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K743009	.	stop_codon	1543	1543	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K743009	.	stem_loop	1517	1536	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_K743009
tcccagcctctcaaccaccgatattgggcccctagctacccctgatattttggcggacattgttgcgcaagtagaacaa
accattgctgctggagcccactgtcgttgtggcggccaagccctagaccaaccgggaaattactatcctcccaccctgc
tcaccgacgttccccccaacgcccctacctaccgtcaggaattttttggccccgtggccctcggatttactgtcgataa
tttagaggaggcgatcgccttggccaatgacattccctttgggttgggggccagtgcatggacaactaacccggaaaat
caacaaaaactaatccggggcatagaagccggggctgtattcattaacggtatgactaaatctgacccccgcattccct
ttggaggcatcaagcgctctggctttgggcgggaacttggccgcatgggcattttagaatttgtcaatgctaaaaccgt
ttggattgcttagtccgccaccattttaaaattatcgctcttctattcgttacgcaggtcttgcctagggggggggagg
ccgtattatcttctagtgatgtttgctgaaaacgcctatctgtgcaaggtttaacatcgttattatgaagcgaaaacta
attcccttttttacgcttcctctattacactattctgcataggaaacccttaatagttcattgtcgagcgaggagaacc
ctgcatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcac
gagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtg
gtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctga
catcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgtt
gttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgt
ccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctct
gaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggct
aaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacacca
tcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgcc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttattagaaaaactcatcgagc
atcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggag
aaaactcaccgaggcagttccaaagaatggcaaggtcctggtaacggtctgcgattccgacccgtccaacatcaataca
acctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaat
ggcaagagcttgtgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaa
ccaaaccgttattcatgcgtgattgcgcctgagcaagacgaaatacacgatcgctgttaaaaggacaattacaaacagg
aatcgaatgtaaccggcgcaggaacacggccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacc
tggaaggctgttttcccaggaatcgcggtggtgagtaaccacgcatcatcaggagtacggataaaatgcttgatggtcg
ggagaggcataaactccgtcagccagttgagacggaccatctcatctgtaacatcattggcaacgctacctttgccatg
tttcagaaacaactctggcgcatcgggcttcccatacaagcgatagattgtcgcacctgattgcccgacattatcgcga
gcccatttatacccatataaatcagcgtccatgttggagtttaagcgcggacgggagcaagacgtttcccgttgaatat
ggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgc
aatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagagt
gtaggcggtaaagtctatgggaacttccagtttaagggctgtcccgttaaatatggcttcgagtttttgaagcataatt
ttctaacgtctgtggatttttttaagcatagcttgaccttctgttggtcgaattctagggattttcatccatattcggt
tcatagggcagctcaatggtgaacattgctcccccagtttcatggttagctgcggttaaatttcccctgtgggccagga
caatttccttggcgatcgccaaacccaggccactgccatggcgttgactatcgggatgtaggcgagcacgggaactatc
accccgataaagtcgctcaaaaatgtagggtaaatcccttggttgaaatcctaggccttgatccctaataatcatggtg
atcccttctgtgccttgatgaccctgcacaaaaatagtcccttccggtgggctatatttcagcgtattgtccaaaatat
tcatcaatacctgcatcagacgatcgccatccccctctaggttcaacttggttggcccttggtaatccagggtgatatt
ttttaccacggcgatcggggttaatcgctcccaggtaatggcgagtaaatggcgtaaattaatcggttctggctcgagg
taaagattgggattagcggttatttgggttaaatggagccaactttccacca