psb1C3_int
type: ComponentDefinition
persistent identity: BBa K743009
display id: BBa_K743009
version: 1
was generated by: iGEM to SBOL conversion
description: psb1C3_IntKR recombination plasmid with RFP under psaAB promoter
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:13:09Z
mutable provenance: Go to http://partsregistry.org/Part:BBa_K743008 for information about the plasmid backbone. Both the psaAB promoter and the RFP coding sequence were obtained from the registry.
owned by: james
owned by: myers
mutable description: The http://partsregistry.org/Part:BBa_K743008 psb1C3_IntKR recombination plasmid allows the neutral integration of any desired sequence into Synechocystis PCC6803 chromosome. Composite part BBa_K743009 is designed for the expression of RFP under the control of the Synechocystis psaAB operon promoter.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _994_
m user id: 0
owner id: 11584
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K743001
component: BBa_S05073
component: BBa_S05075
component: BBa_K743000
sequence annotation: BBa_K743000
sequence annotation: BBa_S05075
sequence annotation: BBa_S05073
sequence annotation: BBa_K743001
sequence: https://synbiohub.org/public/igem/BBa_K743009_sequence/1

BBa_K743001
type: Component
persistent identity: component2269238
display id: component2269238
version: 1
top level: psb1C3_int
definition: RS2
access: public

BBa_S05073
type: Component
persistent identity: component2269236
display id: component2269236
version: 1
top level: psb1C3_int
definition: BBa_S05073
access: public

BBa_S05075
type: Component
persistent identity: component2269224
display id: component2269224
version: 1
top level: psb1C3_int
definition: BBa_S05075
access: public

BBa_K743000
type: Component
persistent identity: component2269214
display id: component2269214
version: 1
top level: psb1C3_int
definition: RS1
access: public

BBa_K743000
type: SequenceAnnotation
persistent identity: annotation2269214
display id: annotation2269214
version: 1
top level: psb1C3_int
location: https://synbiohub.org/public/igem/BBa_K743009/annotation2269214/range2269214/1
component: BBa_K743000

https://synbiohub.org/public/igem/BBa_K743009/annotation2269214/range2269214/1
type: Range
persistent identity: range2269214
display id: range2269214
version: 1
top level: psb1C3_int
direction: direction/reverse
start: 1
end: 522
orientation: inline

BBa_S05075
type: SequenceAnnotation
persistent identity: annotation2269224
display id: annotation2269224
version: 1
top level: psb1C3_int
location: https://synbiohub.org/public/igem/BBa_K743009/annotation2269224/range2269224/1
component: BBa_S05075

https://synbiohub.org/public/igem/BBa_K743009/annotation2269224/range2269224/1
type: Range
persistent identity: range2269224
display id: range2269224
version: 1
top level: psb1C3_int
direction: direction/reverse
start: 523
end: 1421
orientation: inline

BBa_S05073
type: SequenceAnnotation
persistent identity: annotation2269236
display id: annotation2269236
version: 1
top level: psb1C3_int
location: https://synbiohub.org/public/igem/BBa_K743009/annotation2269236/range2269236/1
component: BBa_S05073

https://synbiohub.org/public/igem/BBa_K743009/annotation2269236/range2269236/1
type: Range
persistent identity: range2269236
display id: range2269236
version: 1
top level: psb1C3_int
direction: direction/reverse
start: 1422
end: 2525
orientation: inline

BBa_K743001
type: SequenceAnnotation
persistent identity: annotation2269238
display id: annotation2269238
version: 1
top level: psb1C3_int
location: https://synbiohub.org/public/igem/BBa_K743009/annotation2269238/range2269238/1
component: BBa_K743001

https://synbiohub.org/public/igem/BBa_K743009/annotation2269238/range2269238/1
type: Range
persistent identity: range2269238
display id: range2269238
version: 1
top level: psb1C3_int
direction: direction/reverse
start: 2526
end: 3212
orientation: inline

BBa_S05075
type: ComponentDefinition
persistent identity: BBa S05075
display id: BBa_S05075
version: 1
was generated by: iGEM to SBOL conversion
description: K390016:E1010
created: 2012-09-21T11:00:00Z
modified: 2015-05-08T01:14:49Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 11584
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Juan Sim??n ??lamos
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_E1010
component: BBa_K390016
sequence annotation: BBa_K390016
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_S05075_sequence/1

BBa_E1010
type: Component
persistent identity: component2245002
display id: component2245002
version: 1
top level: BBa_S05075
definition: mRFP1
access: public

BBa_K390016
type: Component
persistent identity: component2244999
display id: component2244999
version: 1
top level: BBa_S05075
definition: BBa_K390016
access: public

BBa_K390016
type: SequenceAnnotation
persistent identity: annotation2244999
display id: annotation2244999
version: 1
top level: BBa_S05075
location: https://synbiohub.org/public/igem/BBa_S05075/annotation2244999/range2244999/1
component: BBa_K390016

https://synbiohub.org/public/igem/BBa_S05075/annotation2244999/range2244999/1
type: Range
persistent identity: range2244999
display id: range2244999
version: 1
top level: BBa_S05075
direction: direction/reverse
start: 1
end: 193
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2245002
display id: annotation2245002
version: 1
top level: BBa_S05075
location: https://synbiohub.org/public/igem/BBa_S05075/annotation2245002/range2245002/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_S05075/annotation2245002/range2245002/1
type: Range
persistent identity: range2245002
display id: range2245002
version: 1
top level: BBa_S05075
direction: direction/reverse
start: 194
end: 899
orientation: inline

RS1
type: ComponentDefinition
persistent identity: BBa K743000
display id: BBa_K743000
version: 1
was generated by: iGEM to SBOL conversion
description: Synechocystis PCC6803 neutral recombination site.
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:09Z
mutable provenance: PCR amplified from synechocystis PCC6803 chromosome.
owned by: james
owned by: myers
mutable description: Any DNA sequence flacked by this part and BBa_K743001 will be incorporated into Synechocistys PCC6803 chromosome through a neutral double homologous recombination.
discontinued: false
dominant: false
experience: experience/Works
group u list: _994_
m user id: 0
owner id: 11584
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: According to literature, in Synechocystis the recombination sites must be at least 450bp long to allow a rasonably high transformation efficiency, being 700bp the optimum size.
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RS1 recombination site
sequence: https://synbiohub.org/public/igem/BBa_K743000_sequence/1

RS1 recombination site
type: SequenceAnnotation
persistent identity: annotation2188518
display id: annotation2188518
version: 1
top level: RS1
location: https://synbiohub.org/public/igem/BBa_K743000/annotation2188518/range2188518/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K743000/annotation2188518/range2188518/1
type: Range
persistent identity: range2188518
display id: range2188518
version: 1
top level: RS1
direction: direction/forward
start: 1
end: 522
orientation: inline

RS2
type: ComponentDefinition
persistent identity: BBa K743001
display id: BBa_K743001
version: 1
was generated by: iGEM to SBOL conversion
description: Synechocystis PCC6803 neutral recombination site.
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:09Z
mutable provenance: PCR amplified from Synechocystis PCC6803 chromosome, orf slr0337
owned by: james
owned by: myers
mutable description: When placed between this part and BBa_K743000, any DNA sequence will be inserted into Synechocystis PCC6803 chromosome trough homologous recombination
discontinued: false
dominant: false
experience: experience/None
group u list: _994_
m user id: 0
owner id: 11584
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size.
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RS2
sequence: https://synbiohub.org/public/igem/BBa_K743001_sequence/1

RS2
type: SequenceAnnotation
persistent identity: annotation2188524
display id: annotation2188524
version: 1
top level: RS2
location: https://synbiohub.org/public/igem/BBa_K743001/annotation2188524/range2188524/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K743001/annotation2188524/range2188524/1
type: Range
persistent identity: range2188524
display id: range2188524
version: 1
top level: RS2
direction: direction/forward
start: 1
end: 687
orientation: inline

BBa_S05073
type: ComponentDefinition
persistent identity: BBa S05073
display id: BBa_S05073
version: 1
was generated by: iGEM to SBOL conversion
description: B0015:P1007
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:14:49Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 11584
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Juan Sim??n ??lamos
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_P1007
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_P1007
sequence: https://synbiohub.org/public/igem/BBa_S05073_sequence/1

BBa_P1007
type: Component
persistent identity: component2188873
display id: component2188873
version: 1
top level: BBa_S05073
definition: KanR
access: public

BBa_B0015
type: Component
persistent identity: component2188869
display id: component2188869
version: 1
top level: BBa_S05073
definition: BBa_B0015
access: public

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2188869
display id: annotation2188869
version: 1
top level: BBa_S05073
location: https://synbiohub.org/public/igem/BBa_S05073/annotation2188869/range2188869/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_S05073/annotation2188869/range2188869/1
type: Range
persistent identity: range2188869
display id: range2188869
version: 1
top level: BBa_S05073
direction: direction/reverse
start: 1
end: 129
orientation: inline

BBa_P1007
type: SequenceAnnotation
persistent identity: annotation2188873
display id: annotation2188873
version: 1
top level: BBa_S05073
location: https://synbiohub.org/public/igem/BBa_S05073/annotation2188873/range2188873/1
component: BBa_P1007

https://synbiohub.org/public/igem/BBa_S05073/annotation2188873/range2188873/1
type: Range
persistent identity: range2188873
display id: range2188873
version: 1
top level: BBa_S05073
direction: direction/reverse
start: 138
end: 1104
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

KanR
type: ComponentDefinition
persistent identity: BBa P1007
display id: BBa_P1007
version: 1
was generated by: iGEM to SBOL conversion
description: kanamycin resistance cassette
created: 2006-02-03T12:00:00Z
modified: 2015-05-08T01:14:11Z
owned by: james
owned by: myers
mutable description: This contains KanR, a terminator and then AmpR and a terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: kanamycin resistance
sequence annotation: G to C mutation to eliminate XhoI site
sequence annotation: repeat region
sequence: https://synbiohub.org/public/igem/BBa_P1007_sequence/1

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation1919399
display id: annotation1919399
version: 1
top level: KanR
location: https://synbiohub.org/public/igem/BBa_P1007/annotation1919399/range1919399/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_P1007/annotation1919399/range1919399/1
type: Range
persistent identity: range1919399
display id: range1919399
version: 1
top level: KanR
direction: direction/reverse
start: 1
end: 819
orientation: inline

G to C mutation to eliminate XhoI site
type: SequenceAnnotation
persistent identity: annotation1919400
display id: annotation1919400
version: 1
top level: KanR
location: https://synbiohub.org/public/igem/BBa_P1007/annotation1919400/range1919400/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_P1007/annotation1919400/range1919400/1
type: Range
persistent identity: range1919400
display id: range1919400
version: 1
top level: KanR
direction: direction/forward
start: 787
end: 787
orientation: inline

repeat region
type: SequenceAnnotation
persistent identity: annotation1919401
display id: annotation1919401
version: 1
top level: KanR
location: https://synbiohub.org/public/igem/BBa_P1007/annotation1919401/range1919401/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_P1007/annotation1919401/range1919401/1
type: Range
persistent identity: range1919401
display id: range1919401
version: 1
top level: KanR
direction: direction/reverse
start: 924
end: 967
orientation: inline

BBa_K390016
type: ComponentDefinition
persistent identity: BBa K390016
display id: BBa_K390016
version: 1
was generated by: iGEM to SBOL conversion
description: psaAB operon promoter, 5'UTR, and RBS
created: 2010-08-01T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: (coming soon)
owned by: james
owned by: myers
mutable description: (coming soon)
discontinued: false
dominant: false
group u list: _518_
m user id: 0
owner id: 6788
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: (coming soon)
star: false
creator: Cody Tramp
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: psaAB operon promoter
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: +1 Transcription Start Point(s)
sequence annotation: rbs region (non exact)
sequence: https://synbiohub.org/public/igem/BBa_K390016_sequence/1

psaAB operon promoter
type: SequenceAnnotation
persistent identity: annotation2076598
display id: annotation2076598
version: 1
top level: BBa_K390016
location: https://synbiohub.org/public/igem/BBa_K390016/annotation2076598/range2076598/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K390016/annotation2076598/range2076598/1
type: Range
persistent identity: range2076598
display id: range2076598
version: 1
top level: BBa_K390016
direction: direction/forward
start: 9
end: 47
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2076601
display id: annotation2076601
version: 1
top level: BBa_K390016
location: https://synbiohub.org/public/igem/BBa_K390016/annotation2076601/range2076601/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K390016/annotation2076601/range2076601/1
type: Range
persistent identity: range2076601
display id: range2076601
version: 1
top level: BBa_K390016
direction: direction/forward
start: 13
end: 18
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2076602
display id: annotation2076602
version: 1
top level: BBa_K390016
location: https://synbiohub.org/public/igem/BBa_K390016/annotation2076602/range2076602/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K390016/annotation2076602/range2076602/1
type: Range
persistent identity: range2076602
display id: range2076602
version: 1
top level: BBa_K390016
direction: direction/forward
start: 35
end: 40
orientation: inline

+1 Transcription Start Point(s)
type: SequenceAnnotation
persistent identity: annotation2076599
display id: annotation2076599
version: 1
top level: BBa_K390016
location: https://synbiohub.org/public/igem/BBa_K390016/annotation2076599/range2076599/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K390016/annotation2076599/range2076599/1
type: Range
persistent identity: range2076599
display id: range2076599
version: 1
top level: BBa_K390016
direction: direction/forward
start: 48
end: 50
orientation: inline

rbs region (non exact)
type: SequenceAnnotation
persistent identity: annotation2076600
display id: annotation2076600
version: 1
top level: BBa_K390016
location: https://synbiohub.org/public/igem/BBa_K390016/annotation2076600/range2076600/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K390016/annotation2076600/range2076600/1
type: Range
persistent identity: range2076600
display id: range2076600
version: 1
top level: BBa_K390016
direction: direction/forward
start: 177
end: 185
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K743009_sequence/1
type: Sequence
persistent identity: BBa K743009 sequence
display id: BBa_K743009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccagcctctcaaccaccgatattgggcccctagctacccctgatattttggcggacattgttgcgcaagtagaacaaaccattgctgctggagcccactgtcgttgtggcggccaagccctagaccaaccgggaaattactatcctcccaccctgctcaccgacgttccccccaacgcccctacctaccgtcaggaattttttggccccgtggccctcggatttactgtcgataatttagaggaggcgatcgccttggccaatgacattccctttgggttgggggccagtgcatggacaactaacccggaaaatcaacaaaaactaatccggggcatagaagccggggctgtattcattaacggtatgactaaatctgacccccgcattccctttggaggcatcaagcgctctggctttgggcgggaacttggccgcatgggcattttagaatttgtcaatgctaaaaccgtttggattgcttagtccgccaccattttaaaattatcgctcttctattcgttacgcaggtcttgcctagggggggggaggccgtattatcttctagtgatgtttgctgaaaacgcctatctgtgcaaggtttaacatcgttattatgaagcgaaaactaattcccttttttacgcttcctctattacactattctgcataggaaacccttaatagttcattgtcgagcgaggagaaccctgcatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgcccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccaaagaatggcaaggtcctggtaacggtctgcgattccgacccgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaagagcttgtgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcatgcgtgattgcgcctgagcaagacgaaatacacgatcgctgttaaaaggacaattacaaacaggaatcgaatgtaaccggcgcaggaac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05075_sequence/1
type: Sequence
persistent identity: BBa S05075 sequence
display id: BBa_S05075_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttacgcaggtcttgcctagggggggggaggccgtattatcttctagtgatgtttgctgaaaacgcctatctgtgcaaggtttaacatcgttattatgaagcgaaaactaattcccttttttacgcttcctctattacactattctgcataggaaacccttaatagttcattgtcgagcgaggagaaccctgcatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K743000_sequence/1
type: Sequence
persistent identity: BBa K743000 sequence
display id: BBa_K743000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccagcctctcaaccaccgatattgggcccctagctacccctgatattttggcggacattgttgcgcaagtagaacaaaccattgctgctggagcccactgtcgttgtggcggccaagccctagaccaaccgggaaattactatcctcccaccctgctcaccgacgttccccccaacgcccctacctaccgtcaggaattttttggccccgtggccctcggatttactgtcgataatttagaggaggcgatcgccttggccaatgacattccctttgggttgggggccagtgcatggacaactaacccggaaaatcaacaaaaactaatccggggcatagaagccggggctgtattcattaacggtatgactaaatctgacccccgcattccctttggaggcatcaagcgctctggctttgggcgggaacttggccgcatgggcattttagaatttgtcaatgctaaaaccgtttggattgcttagtccgccaccattttaaaattatcgctcttctattc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K743001_sequence/1
type: Sequence
persistent identity: BBa K743001 sequence
display id: BBa_K743001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtgtaggcggtaaagtctatgggaacttccagtttaagggctgtcccgttaaatatggcttcgagtttttgaagcataattttctaacgtctgtggatttttttaagcatagcttgaccttctgttggtcgaattctagggattttcatccatattcggttcatagggcagctcaatggtgaacattgctcccccagtttcatggttagctgcggttaaatttcccctgtgggccaggacaatttccttggcgatcgccaaacccaggccactgccatggcgttgactatcgggatgtaggcgagcacgggaactatcaccccgataaagtcgctcaaaaatgtagggtaaatcccttggttgaaatcctaggccttgatccctaataatcatggtgatcccttctgtgccttgatgaccctgcacaaaaatagtcccttccggtgggctatatttcagcgtattgtccaaaatattcatcaatacctgcatcagacgatcgccatccccctctaggttcaacttggttggcccttggtaatccagggtgatattttttaccacggcgatcggggttaatcgctcccaggtaatggcgagtaaatggcgtaaattaatcggttctggctcgaggtaaagattgggattagcggttatttgggttaaatggagccaactttccacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05073_sequence/1
type: Sequence
persistent identity: BBa S05073 sequence
display id: BBa_S05073_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccaaagaatggcaaggtcctggtaacggtctgcgattccgacccgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaagagcttgtgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcatgcgtgattgcgcctgagcaagacgaaatacacgatcgctgttaaaaggacaattacaaacaggaatcgaatgtaaccggcgcaggaacacggccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaaggctgttttcccaggaatcgcggtggtgagtaaccacgcatcatcaggagtacggataaaatgcttgatggtcgggagaggcataaactccgtcagccagttgagacggaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaagcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcgtccatgttggagtttaagcgcggacgggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1007_sequence/1
type: Sequence
persistent identity: BBa P1007 sequence
display id: BBa_P1007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccaaagaatggcaaggtcctggtaacggtctgcgattccgacccgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaagagcttgtgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcatgcgtgattgcgcctgagcaagacgaaatacacgatcgctgttaaaaggacaattacaaacaggaatcgaatgtaaccggcgcaggaacacggccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaaggctgttttcccaggaatcgcggtggtgagtaaccacgcatcatcaggagtacggataaaatgcttgatggtcgggagaggcataaactccgtcagccagttgagacggaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaagcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcgtccatgttggagtttaagcgcggacgggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K390016_sequence/1
type: Sequence
persistent identity: BBa K390016 sequence
display id: BBa_K390016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttacgcaggtcttgcctagggggggggaggccgtattatcttctagtgatgtttgctgaaaacgcctatctgtgcaaggtttaacatcgttattatgaagcgaaaactaattcccttttttacgcttcctctattacactattctgcataggaaacccttaatagttcattgtcgagcgaggagaaccctgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z