LOCUS BBa_K743010 5032 bp DNA linear 16-Feb-2025 DEFINITION psb1C3_IntS recombination plasmid with RFP ACCESSION BBa_K743010 VERSION BBa_K743010.1 FEATURES Location/Qualifiers promoter 1..219 /label=Spectinomycin resistance promoter CDS 220..1011 /label=Spectinomycin resistance primer_bind 1012..1031 /label=Verification forward primer binding site stem_loop 1032..1110 /label=B0055 terminator misc_feature 1111..1121 /label=three stop codons misc_feature 1112..1156 /label=TOPO A cloning site BBa_B0043 promoter 1157..1356 /label=lacI promoter CDS 1383..2057 /label=BBa_E1010 mRFP misc_feature 2058..2063 /label=stop codons stem_loop 2097..2225 /label=B0015 double terminator misc_feature 2226..2246 /label=biobrick Suffix misc_feature 1135..1156 /label=Biobrick Prefix misc_feature 2247..2259 /label=TOPO A cloning site misc_feature 2260..2271 /label=three stop codons stem_loop 2272..2340 /label=B0054 terminator primer_bind 2341..2360 /label=Verification revers primer binding site misc_feature 2366..2832 /label=5?? recombination site misc_feature 4589..5032 /label=3' recombination site misc_feature 2833..4588 /label=plasmid backbone ORIGIN 1 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg 61 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc 121 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt 181 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc 241 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg 301 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat 361 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc 421 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa 481 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg 541 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc 601 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt 661 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat 721 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg 781 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc 841 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct 901 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa 961 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata atgccacctg 1021 acgtctaaga aaaggaatat tcagcaattt gcccgtgccg aagaaaggcc cacccgtgaa 1081 ggtgagccag tgagttgatt gctacgtaat tagttagtta gcccttagtg actcgaattc 1141 gcggccgctt ctagagcaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 1201 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 1261 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 1321 ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagaaagag gagaaatact 1381 agatggcttc ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag 1441 gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag 1501 gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca 1561 tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc 1621 cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg 1681 aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct 1741 acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa 1801 ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg 1861 aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca 1921 cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg 1981 acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc 2041 gtcactccac cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag 2101 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2161 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 2221 ttatatacta gtagcggccg ctgcaggagt cactaagggt tagttagtta gattagcaga 2281 aagtcaaaag cctccgaccg gaggcttttg actaaaactt cccttggggt tatcattggg 2341 gctcactcaa aggcggtaat cagatgctga tattgcccac gaattacgta caccgattac 2401 ggcgattcag gcaacgttgg aaacgacatt aaatgcagaa cctaatgctg aagaaaccca 2461 tagcaccttg caaaccttaa aacgacaaaa ttatcgtctc agccatttaa tccatgattt 2521 attactcctc tcccgcatgg atttaacaac agttaatccg acccagttta ccctttgttg 2581 tctcaatgat ttagttgaag atttaaccga agaatttgcc agtttggcga tcgccgctgg 2641 ggtattgtta tctgcgaagt tagacaatca agcaaatatc tgggtcaggg gagaagaaga 2701 acagctttat cgtttggtgg gtaatttaat cagtaatgcc attcactaca ccccaacggg 2761 gggcgaggta accgtcatgc ttgagaccga taaacagcaa gccatcatca aagtccaaga 2821 tacgggcatt ggggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 2881 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc 2941 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 3001 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 3061 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 3121 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 3181 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 3241 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 3301 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 3361 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 3421 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 3481 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 3541 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 3601 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 3661 gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca ataaaaaacg 3721 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat 3781 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg 3841 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga 3901 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg 3961 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa 4021 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag 4081 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa 4141 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg 4201 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat 4261 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc 4321 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag 4381 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata 4441 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat 4501 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa 4561 cctcttacgt gcccgatcaa ctcgagtgca aattttgcct aatctttgct taggtacagt 4621 agcttgccct aggtcgcctc aacggaggca tattctcaac gcaacccaac agccgggcta 4681 ttatgtccgg tttttggact taaacggtca attattagca acggcagggg aaaatccccc 4741 aggcttagcc tttgagcggg aaaccacccg tcaaaaacca ttaacggatc aaaaagggaa 4801 ccgctatcac caagtttctt tgctgttaaa aaccaccact ggtgaacctt ggggctattt 4861 aaaggtgggg cgctccttgg tggaatatga ccatcatttg cacacgatcc aaggcttttt 4921 agtttggggt ttacccattg tgatgattgt ggtcggtggt gctagttggt ggttagcagg 4981 cttagccatg gagccggttt atcgttccta ccaacaaatc cagcagttca cc //