LOCUS       BBa_K743012             2587 bp    DNA     linear       22-Dec-2024
DEFINITION  OmpA + HIV protease cleavage site - GFP secretion system
ACCESSION   BBa_K743012
VERSION     BBa_K743012.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1211..1217
                     /label=NdeI
     CDS             1214..1648
                     /label=OmpA
     CDS             1649..1674
                     /label=linker
     misc_feature    1669..1674
                     /label=SacI
     misc_feature    1214..1216
                     /label=ATG
     misc_feature    1211..1674
                     /label=BBa_K103006
     misc_feature    1..1674
                     /label=BBa_S05076
     misc_feature    1683..1724
                     /label=BBa_I712015
     misc_feature    1731..1733
                     /label=start
     CDS             1731..2450
                     /label=superfolder GFP coding region
     misc_feature    2445..2450
                     /label=stop
     CDS             1731..2450
                     /label=BBa_I746916
     stem_loop       2470..2513
                     /label=stem_loop
     misc_feature    2459..2538
                     /label=BBa_B0010
     terminator      2459..2538
                     /label=BBa_B0010
     stem_loop       2554..2573
                     /label=T7 TE
     misc_feature    2580..2580
                     /label=stop
     polyA_site      2574..2587
                     /label=polya
     misc_feature    2547..2587
                     /label=BBa_B0012
     terminator      2547..2587
                     /label=BBa_B0012
     terminator      2459..2587
                     /label=BBa_B0015
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc catatgaaag ctactaaact ggtactgggc gcggtaatcc tgggttctac
     1261 tctgctggca ggttgctcca gcaacgctaa aatcgatcag ggaattaacc cgtatgttgg
     1321 ctttgaaatg ggttacgact ggttaggtcg tatgccgtac aaaggcagcg ttgaaaacgg
     1381 tgcatacaaa gctcagggcg ttcaactgac cgctaaactg ggttacccaa tcactgacga
     1441 cctggacatc tacactcgtc tgggtggcat ggtatggcgt gcagacacta aatccaacgt
     1501 ttatggtaaa aaccacgaca ccggcgtttc tccggtcttc gctggcggtg ttgagtacgc
     1561 gatcactcct gaaatcgcta cccgtctgga ataccagtgg accaacaaca tcggtgacgc
     1621 acacaccatc ggcactcgtc cggacaacgg cggaggttct ggaggaggga gctctactag
     1681 agagccaggt gagccagaac taccccatcg tgcagaacct ccaatactag atgcgtaaag
     1741 gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt gatgtcaacg
     1801 gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt aaactgacgc
     1861 tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg gtaacgacgc
     1921 tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag catgacttct
     1981 tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt aaggatgacg
     2041 gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta aaccgcattg
     2101 agctgaaagg cattgacttt aaagaagacg gcaatatcct gggccataag ctggaataca
     2161 attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc attaaagcga
     2221 attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat cactaccagc
     2281 aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat ctgagcacgc
     2341 aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg ctggagttcg
     2401 taaccgcagc gggcatcacg catggtatgg atgaactgta caaatgatga tactagagcc
     2461 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2521 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2581 gtttata
//