LOCUS       BBa_K743015             2234 bp    DNA     linear       28-Jun-2024
DEFINITION  after dusk -> LuxAB luciferase (Vibrio fischerii)
ACCESSION   BBa_K743015
VERSION     BBa_K743015.1  
FEATURES             Location/Qualifiers
     promoter        1..150
                     /label=Pta BBa_K743003 promoter
     RBS             120..150
                     /label=putative endogenous rbs
     CDS             151..1212
                     /label=LuxA v. fischeri
     RBS             1216..1253
                     /label=rbs
     misc_feature    1213..1216
                     /label=stop codon
     CDS             1254..2231
                     /label=LuxB v. fischeri
     misc_feature    2232..2234
                     /label=stop codon
ORIGIN
        1 aacgcatcgg cgttccctag cttaaattat cttgatgtcc aaagaaggct cgctttcggg
       61 aatcaccatt accattggag ggaaagacgt tgaacttgtt taatcgccat tatgggtaag
      121 aatctcctcg aacaactgcg ccaatttaca atgaagtttg gaaatatttg tttttcgtat
      181 caaccaccag gtgaaactca taagcaagta atggatcgct ttgttcggct tggtatcgcc
      241 tcagaagagg tagggtttga tacatattgg accttagaac atcattttac agagtttggt
      301 cttacgggaa atttatttgt tgctgcggct aacctgttag gaagaactaa aacattaaat
      361 gttggcacta tgggggttgt tattccgaca gcacacccag ttcgacagtt agaagacgtt
      421 ttattattag atcaaatgtc gaaaggtcgt tttaattttg gaaccgttcg agggctatac
      481 cataaagatt ttcgagtatt tggtgttgat atggaagagt ctcgagcaat tactcaaaat
      541 ttctaccaga tgataatgga aagcttacag acaggaacca ttagctctga tagtgattac
      601 attcaatttc ctaaggttga tgtatatccc aaagtgtact caaaaaatgt accaacctgt
      661 atgactgctg agtccgcaag tacgacagaa tggctagcaa tacaagggct accaatggtt
      721 cttagttgga ttattggtac taatgaaaaa aaagcacaga tggaactcta taatgaaatt
      781 gcgacagaat atggtcatga tatatctaaa atagatcatt gtatgactta tatttgttct
      841 gttgatgatg atgcacaaaa ggcgcaagat gtttgtcggg agtttctgaa aaattggtat
      901 gactcatatg taaatgcgac caatatcttt aatgatagca atcaaactcg tggttatgat
      961 tatcataaag gtcaatggcg tgattttgtt ttacaaggac atacaaacac caatcgacgt
     1021 gttgattata gcaatggtat taaccctgta ggcactcctg agcagtgtat tgaaatcatt
     1081 caacgtgata ttgatgcaac gggtattaca aacattacat gcggatttga agctaatgga
     1141 actgaagatg aaataattgc ttccatgcga cgctttatga cacaagtcgc tcctttctta
     1201 aaagaaccta aataaattac ttatttgata ctagagataa taaggaacaa gttatgaaat
     1261 ttggattatt ttttctaaac tttcagaaag atggaataac atctgaagaa acgttggata
     1321 atatggtaaa gactgtcacg ttaattgatt caactaaata tcattttaat actgcctttg
     1381 ttaatgaaca tcacttttca aaaaatggta ttgttggagc acctattacc gcagctggtt
     1441 ttttattagg gttaacaaat aaattacata ttggttcatt aaatcaagta attaccaccc
     1501 atcaccctgt acgtgtagca gaagaagcca gtttattaga tcaaatgtca gagggacgct
     1561 tcattcttgg ttttagtgac tgcgaaagtg atttcgaaat ggaatttttt agacgtcata
     1621 tctcatcaag gcaacaacaa tttgaagcat gctatgaaat aattaatgac gcattaacta
     1681 caggttattg tcatccccaa aacgactttt atgattttcc aaaggtttca attaatccac
     1741 actgttacag tgagaatgga cctaagcaat atgtatccgc tacatcaaaa gaagtcgtca
     1801 tgtgggcagc gaaaaaggca ctgcctttaa catttaagtg ggaggataat ttagaaacca
     1861 aagaacgcta tgcaattcta tataataaaa cagcacaaca atatggtatt gatatttcgg
     1921 atgttgatca tcaattaact gtaattgcga acttaaatgc tgatagaagt acggctcaag
     1981 aagaagtgag agaatactta aaagactata tcactgaaac ttaccctcaa atggacagag
     2041 atgaaaaaat taactgcatt attgaagaga atgcagttgg gtctcatgat gactattatg
     2101 aatcgacaaa attagcagtg gaaaaaacag ggtctaaaaa tattttatta tcctttgaat
     2161 caatgtccga tattaaagat gtaaaagata ttattgatat gttgaaccaa aaaatcgaaa
     2221 tgaatttacc ataa
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