BBa_K743015 1 BBa_K743015 after dusk -> LuxAB luciferase (Vibrio fischerii) 2012-09-21T11:00:00Z 2015-05-08T01:13:09Z Pta promoter was amplified from genomic Synechocystis DNA, you can find a biobrick version of this promoter at blahblahblah. LuxAB genes were obtained from the Luxbrick This part is designed for the expression of the LuxA and LuxB genes from Vibrio fischeri in a circadian manner, peaking a 2 hours after subjective dusk (or hour 14 of circadian time if you prefer). When transfromed into Syenchocystis PCC6803, the endogenous circadian transcriptional mechanism controls the activity of the Pta promoter making the heterodimeric LuxAB luciferase to be expressed at the specified time. This permits for the emition of light by the luciferase reaction to be restricted to this time of the day (dependent on the addition or endogenous production of substrates) false false _994_ 0 11584 9 It's complicated false This part was made by Gibson assembly, therefore it has no scars false Bernardo Pollak annotation2193795 1 Pta BBa_K743003 promoter range2193795 1 1 150 annotation2193796 1 putative endogenous rbs range2193796 1 120 150 annotation2193801 1 stop codon range2193801 1 2232 2234 annotation2193797 1 LuxA v. fischeri range2193797 1 151 1212 annotation2193800 1 LuxB v. fischeri range2193800 1 1254 2231 annotation2193799 1 stop codon range2193799 1 1213 1216 annotation2193798 1 rbs range2193798 1 1216 1253 BBa_K743015_sequence 1 aacgcatcggcgttccctagcttaaattatcttgatgtccaaagaaggctcgctttcgggaatcaccattaccattggagggaaagacgttgaacttgtttaatcgccattatgggtaagaatctcctcgaacaactgcgccaatttacaatgaagtttggaaatatttgtttttcgtatcaaccaccaggtgaaactcataagcaagtaatggatcgctttgttcggcttggtatcgcctcagaagaggtagggtttgatacatattggaccttagaacatcattttacagagtttggtcttacgggaaatttatttgttgctgcggctaacctgttaggaagaactaaaacattaaatgttggcactatgggggttgttattccgacagcacacccagttcgacagttagaagacgttttattattagatcaaatgtcgaaaggtcgttttaattttggaaccgttcgagggctataccataaagattttcgagtatttggtgttgatatggaagagtctcgagcaattactcaaaatttctaccagatgataatggaaagcttacagacaggaaccattagctctgatagtgattacattcaatttcctaaggttgatgtatatcccaaagtgtactcaaaaaatgtaccaacctgtatgactgctgagtccgcaagtacgacagaatggctagcaatacaagggctaccaatggttcttagttggattattggtactaatgaaaaaaaagcacagatggaactctataatgaaattgcgacagaatatggtcatgatatatctaaaatagatcattgtatgacttatatttgttctgttgatgatgatgcacaaaaggcgcaagatgtttgtcgggagtttctgaaaaattggtatgactcatatgtaaatgcgaccaatatctttaatgatagcaatcaaactcgtggttatgattatcataaaggtcaatggcgtgattttgttttacaaggacatacaaacaccaatcgacgtgttgattatagcaatggtattaaccctgtaggcactcctgagcagtgtattgaaatcattcaacgtgatattgatgcaacgggtattacaaacattacatgcggatttgaagctaatggaactgaagatgaaataattgcttccatgcgacgctttatgacacaagtcgctcctttcttaaaagaacctaaataaattacttatttgatactagagataataaggaacaagttatgaaatttggattattttttctaaactttcagaaagatggaataacatctgaagaaacgttggataatatggtaaagactgtcacgttaattgattcaactaaatatcattttaatactgcctttgttaatgaacatcacttttcaaaaaatggtattgttggagcacctattaccgcagctggttttttattagggttaacaaataaattacatattggttcattaaatcaagtaattaccacccatcaccctgtacgtgtagcagaagaagccagtttattagatcaaatgtcagagggacgcttcattcttggttttagtgactgcgaaagtgatttcgaaatggaattttttagacgtcatatctcatcaaggcaacaacaatttgaagcatgctatgaaataattaatgacgcattaactacaggttattgtcatccccaaaacgacttttatgattttccaaaggtttcaattaatccacactgttacagtgagaatggacctaagcaatatgtatccgctacatcaaaagaagtcgtcatgtgggcagcgaaaaaggcactgcctttaacatttaagtgggaggataatttagaaaccaaagaacgctatgcaattctatataataaaacagcacaacaatatggtattgatatttcggatgttgatcatcaattaactgtaattgcgaacttaaatgctgatagaagtacggctcaagaagaagtgagagaatacttaaaagactatatcactgaaacttaccctcaaatggacagagatgaaaaaattaactgcattattgaagagaatgcagttgggtctcatgatgactattatgaatcgacaaaattagcagtggaaaaaacagggtctaaaaatattttattatcctttgaatcaatgtccgatattaaagatgtaaaagatattattgatatgttgaaccaaaaaatcgaaatgaatttaccataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z