LOCUS       BBa_K743016             3106 bp    DNA     linear       16-Feb-2025
DEFINITION  psb1C3_IntKR recombination plasmid with RFP under psbA2 promoter
ACCESSION   BBa_K743016
VERSION     BBa_K743016.1  
FEATURES             Location/Qualifiers
     misc_feature    1..522
                     /label=RS1 recombination site
     misc_feature    1..522
                     /label=BBa_K743000
     promoter        523..560
                     /label=psbA2 promoter
     RBS             593..602
                     /label=putative rbs
     misc_feature    525..530
                     /label=-35 box
     misc_feature    549..554
                     /label=-10 box
     misc_feature    561..561
                     /label=+1 Transcription Start Point
     promoter        523..609
                     /label=BBa_K390017
     CDS             610..1284
                     /label=mrfp1
     misc_feature    1291..1315
                     /label=Help:Barcodes
     CDS             610..1315
                     /label=BBa_E1010
     stem_loop       1327..1370
                     /label=stem_loop
     misc_feature    1316..1395
                     /label=BBa_B0010
     terminator      1316..1395
                     /label=BBa_B0010
     stem_loop       1411..1430
                     /label=T7 TE
     misc_feature    1437..1437
                     /label=stop
     polyA_site      1431..1444
                     /label=polya
     misc_feature    1404..1444
                     /label=BBa_B0012
     terminator      1404..1444
                     /label=BBa_B0012
     terminator      1316..1444
                     /label=BBa_B0015
     CDS             1453..2271
                     /label=kanamycin resistance
     misc_feature    2239..2239
                     /label=G to C mutation to eliminate XhoI site
     misc_feature    2376..2419
                     /label=repeat region
     misc_feature    1453..2419
                     /label=BBa_P1007
     misc_feature    1316..2419
                     /label=BBa_S05073
     misc_feature    2420..3106
                     /label=RS2
     misc_feature    2420..3106
                     /label=BBa_K743001
ORIGIN
        1 tcccagcctc tcaaccaccg atattgggcc cctagctacc cctgatattt tggcggacat
       61 tgttgcgcaa gtagaacaaa ccattgctgc tggagcccac tgtcgttgtg gcggccaagc
      121 cctagaccaa ccgggaaatt actatcctcc caccctgctc accgacgttc cccccaacgc
      181 ccctacctac cgtcaggaat tttttggccc cgtggccctc ggatttactg tcgataattt
      241 agaggaggcg atcgccttgg ccaatgacat tccctttggg ttgggggcca gtgcatggac
      301 aactaacccg gaaaatcaac aaaaactaat ccggggcata gaagccgggg ctgtattcat
      361 taacggtatg actaaatctg acccccgcat tccctttgga ggcatcaagc gctctggctt
      421 tgggcgggaa cttggccgca tgggcatttt agaatttgtc aatgctaaaa ccgtttggat
      481 tgcttagtcc gccaccattt taaaattatc gctcttctat tcgctttaca aaactctcat
      541 taatccttta gactaagttt agtcagttcc aatctgaaca tcgacaaata cataaggaat
      601 tataaccaaa tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt
      661 atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg
      721 tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct
      781 tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct
      841 gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg
      901 aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag
      961 ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag
     1021 aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg
     1081 aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt
     1141 aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc
     1201 aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct
     1261 gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgcccagg
     1321 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1381 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1441 tatatactag agttattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
     1501 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     1561 accgaggcag ttccaaagaa tggcaaggtc ctggtaacgg tctgcgattc cgacccgtcc
     1621 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     1681 accatgagtg acgactgaat ccggtgagaa tggcaagagc ttgtgcattt ctttccagac
     1741 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     1801 attcatgcgt gattgcgcct gagcaagacg aaatacacga tcgctgttaa aaggacaatt
     1861 acaaacagga atcgaatgta accggcgcag gaacacggcc agcgcatcaa caatattttc
     1921 acctgaatca ggatattctt ctaatacctg gaaggctgtt ttcccaggaa tcgcggtggt
     1981 gagtaaccac gcatcatcag gagtacggat aaaatgcttg atggtcggga gaggcataaa
     2041 ctccgtcagc cagttgagac ggaccatctc atctgtaaca tcattggcaa cgctaccttt
     2101 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaagcgat agattgtcgc
     2161 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag cgtccatgtt
     2221 ggagtttaag cgcggacggg agcaagacgt ttcccgttga atatggctca taacacccct
     2281 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
     2341 tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt
     2401 tgctgagttg aaggatcaga gtgtaggcgg taaagtctat gggaacttcc agtttaaggg
     2461 ctgtcccgtt aaatatggct tcgagttttt gaagcataat tttctaacgt ctgtggattt
     2521 ttttaagcat agcttgacct tctgttggtc gaattctagg gattttcatc catattcggt
     2581 tcatagggca gctcaatggt gaacattgct cccccagttt catggttagc tgcggttaaa
     2641 tttcccctgt gggccaggac aatttccttg gcgatcgcca aacccaggcc actgccatgg
     2701 cgttgactat cgggatgtag gcgagcacgg gaactatcac cccgataaag tcgctcaaaa
     2761 atgtagggta aatcccttgg ttgaaatcct aggccttgat ccctaataat catggtgatc
     2821 ccttctgtgc cttgatgacc ctgcacaaaa atagtccctt ccggtgggct atatttcagc
     2881 gtattgtcca aaatattcat caatacctgc atcagacgat cgccatcccc ctctaggttc
     2941 aacttggttg gcccttggta atccagggtg atatttttta ccacggcgat cggggttaat
     3001 cgctcccagg taatggcgag taaatggcgt aaattaatcg gttctggctc gaggtaaaga
     3061 ttgggattag cggttatttg ggttaaatgg agccaacttt ccacca
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