##gff-version 3
##sequence-region BBa_K743016 1 3106
BBa_K743016	.	sequence_feature	1	522	.	+	0	ID=BBa_K743000;Name=BBa_K743000
BBa_K743016	.	sequence_feature	1	522	.	+	0	ID=annotation2188518;Name=RS1 recombination site;Parent=BBa_K743000
BBa_K743016	.	promoter	523	609	.	+	0	ID=BBa_K390017;Name=BBa_K390017
BBa_K743016	.	sequence_feature	561	561	.	+	0	ID=annotation2076607;Name=+1 Transcription Start Point;Parent=BBa_K390017
BBa_K743016	.	sequence_feature	525	530	.	+	0	ID=annotation2076605;Name=-35 box;Parent=BBa_K390017
BBa_K743016	.	ribosome_entry_site	593	602	.	+	0	ID=annotation2076604;Name=putative rbs;Parent=BBa_K390017
BBa_K743016	.	sequence_feature	549	554	.	+	0	ID=annotation2076606;Name=-10 box;Parent=BBa_K390017
BBa_K743016	.	promoter	523	560	.	+	0	ID=annotation2076603;Name=psbA2 promoter;Parent=BBa_K390017
BBa_K743016	.	engineered_region	1316	2419	.	+	0	ID=BBa_S05073;Name=BBa_S05073
BBa_K743016	.	engineered_region	1453	2419	.	+	0	ID=BBa_P1007;Name=BBa_P1007;Parent=BBa_S05073
BBa_K743016	.	point_mutation	2239	2239	.	+	0	ID=annotation1919400;Name=G to C mutation to eliminate XhoI site;Parent=BBa_P1007
BBa_K743016	.	CDS	1453	2271	.	+	0	ID=annotation1919399;Name=kanamycin resistance;Parent=BBa_P1007
BBa_K743016	.	sequence_feature	2376	2419	.	+	0	ID=annotation1919401;Name=repeat region;Parent=BBa_P1007
BBa_K743016	.	terminator	1316	1444	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S05073
BBa_K743016	.	terminator	1316	1395	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K743016	.	engineered_region	1316	1395	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K743016	.	stem_loop	1327	1370	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K743016	.	terminator	1404	1444	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K743016	.	stop_codon	1437	1437	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K743016	.	polyA_site	1431	1444	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K743016	.	stem_loop	1411	1430	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K743016	.	engineered_region	1404	1444	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K743016	.	sequence_feature	2420	3106	.	+	0	ID=BBa_K743001;Name=BBa_K743001
BBa_K743016	.	sequence_feature	2420	3106	.	+	0	ID=annotation2188524;Name=RS2;Parent=BBa_K743001
BBa_K743016	.	CDS	610	1315	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K743016	.	engineered_tag	1291	1315	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K743016	.	CDS	610	1284	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
>BBa_K743016
tcccagcctctcaaccaccgatattgggcccctagctacccctgatattttggcggacattgttgcgcaagtagaacaa
accattgctgctggagcccactgtcgttgtggcggccaagccctagaccaaccgggaaattactatcctcccaccctgc
tcaccgacgttccccccaacgcccctacctaccgtcaggaattttttggccccgtggccctcggatttactgtcgataa
tttagaggaggcgatcgccttggccaatgacattccctttgggttgggggccagtgcatggacaactaacccggaaaat
caacaaaaactaatccggggcatagaagccggggctgtattcattaacggtatgactaaatctgacccccgcattccct
ttggaggcatcaagcgctctggctttgggcgggaacttggccgcatgggcattttagaatttgtcaatgctaaaaccgt
ttggattgcttagtccgccaccattttaaaattatcgctcttctattcgctttacaaaactctcattaatcctttagac
taagtttagtcagttccaatctgaacatcgacaaatacataaggaattataaccaaatggcttcctccgaagacgttat
caaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaa
ggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcc
tgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcctt
cccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctg
caagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaa
ccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaa
actgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgct
tacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaag
gtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgcccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttatatactagagttattagaaaaactcatcgagcatcaaatgaaactgcaatttattcata
tcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccaaagaa
tggcaaggtcctggtaacggtctgcgattccgacccgtccaacatcaatacaacctattaatttcccctcgtcaaaaat
aaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaagagcttgtgcatttctttccag
acttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcatgcgtgattgcg
cctgagcaagacgaaatacacgatcgctgttaaaaggacaattacaaacaggaatcgaatgtaaccggcgcaggaacac
ggccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaaggctgttttcccaggaatcgcg
gtggtgagtaaccacgcatcatcaggagtacggataaaatgcttgatggtcgggagaggcataaactccgtcagccagt
tgagacggaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcggg
cttcccatacaagcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcg
tccatgttggagtttaagcgcggacgggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgt
ttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacac
aacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcagagtgtaggcggtaaagtctatgggaacttc
cagtttaagggctgtcccgttaaatatggcttcgagtttttgaagcataattttctaacgtctgtggatttttttaagc
atagcttgaccttctgttggtcgaattctagggattttcatccatattcggttcatagggcagctcaatggtgaacatt
gctcccccagtttcatggttagctgcggttaaatttcccctgtgggccaggacaatttccttggcgatcgccaaaccca
ggccactgccatggcgttgactatcgggatgtaggcgagcacgggaactatcaccccgataaagtcgctcaaaaatgta
gggtaaatcccttggttgaaatcctaggccttgatccctaataatcatggtgatcccttctgtgccttgatgaccctgc
acaaaaatagtcccttccggtgggctatatttcagcgtattgtccaaaatattcatcaatacctgcatcagacgatcgc
catccccctctaggttcaacttggttggcccttggtaatccagggtgatattttttaccacggcgatcggggttaatcg
ctcccaggtaatggcgagtaaatggcgtaaattaatcggttctggctcgaggtaaagattgggattagcggttatttgg
gttaaatggagccaactttccacca