psb1C3_IntKR recombination plasmid with RFP under psbA2 promoter
psbA2 was amplified from Synechocystis genomic DNA and its sequence was obtained from BBa_K390017. RFP is from blubliblo For more information about the plasmid backbone, check [http://partsregistry.org/Part:BBa_K743008 psb1C3_IntKR recombination plasmid] page.
The [http://partsregistry.org/Part:BBa_K743008 psb1C3_IntKR recombination plasmid] allows the neutral integration of any desired sequence into Synechocystis PCC6803 chromosome. Composite part BBa_K743016 is designed for the expression of RFP under the control of the Synechocystis psbA2 promoter.
This composite part is not intended to be used for standard biobrick assembly because of a EcoR1 restriction site in psb1C3_intK plasmid. You should use gibson assembly.
**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
Campbell et al., PNAS v99 p7877 URL
monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
Synechocystis PCC6803 neutral recombination site.
PCR amplified from synechocystis PCC6803 chromosome.
Any DNA sequence flacked by this part and BBa_K743001 will be incorporated into Synechocistys PCC6803 chromosome through a neutral double homologous recombination.
According to literature, in Synechocystis the recombination sites must be at least 450bp long to allow a rasonably high transformation efficiency, being 700bp the optimum size.
PCR amplified from Synechocystis PCC6803 chromosome, orf slr0337
When placed between this part and BBa_K743000, any DNA sequence will be inserted into Synechocystis PCC6803 chromosome trough homologous recombination
According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size.
Double terminator consisting of BBa_B0010 and BBa_B0012
This contains KanR, a terminator and then AmpR and a terminator.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1