LOCUS       BBa_K743017             1083 bp    DNA     linear       22-Dec-2024
DEFINITION  sfGFP under LacI reporter device
ACCESSION   BBa_K743017
VERSION     BBa_K743017.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start
     CDS             227..946
                     /label=superfolder GFP coding region
     misc_feature    941..946
                     /label=stop
     CDS             227..946
                     /label=BBa_I746916
     stem_loop       966..1009
                     /label=stem_loop
     misc_feature    955..1034
                     /label=BBa_B0010
     terminator      955..1034
                     /label=BBa_B0010
     stem_loop       1050..1069
                     /label=T7 TE
     misc_feature    1076..1076
                     /label=stop
     polyA_site      1070..1083
                     /label=polya
     misc_feature    1043..1083
                     /label=BBa_B0012
     terminator      1043..1083
                     /label=BBa_B0012
     terminator      955..1083
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc gtaaaggcga
      241 agagctgttc actggtgtcg tccctattct ggtggaactg gatggtgatg tcaacggtca
      301 taagttttcc gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac tgacgctgaa
      361 gttcatctgt actactggta aactgccggt accttggccg actctggtaa cgacgctgac
      421 ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg aagcagcatg acttcttcaa
      481 gtccgccatg ccggaaggct atgtgcagga acgcacgatt tcctttaagg atgacggcac
      541 gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc ctggtaaacc gcattgagct
      601 gaaaggcatt gactttaaag aagacggcaa tatcctgggc cataagctgg aatacaattt
      661 taacagccac aatgtttaca tcaccgccga taaacaaaaa aatggcatta aagcgaattt
      721 taaaattcgc cacaacgtgg aggatggcag cgtgcagctg gctgatcact accagcaaaa
      781 cactccaatc ggtgatggtc ctgttctgct gccagacaat cactatctga gcacgcaaag
      841 cgttctgtct aaagatccga acgagaaacg cgatcatatg gttctgctgg agttcgtaac
      901 cgcagcgggc atcacgcatg gtatggatga actgtacaaa tgatgatact agagccaggc
      961 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1021 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1081 ata
//