LOCUS       BBa_K743018             3254 bp    DNA     linear       25-Nov-2024
DEFINITION  sfGFP.degradation.tag under Pta promoter in psb1C3_IntKR plasmid
ACCESSION   BBa_K743018
VERSION     BBa_K743018.1  
FEATURES             Location/Qualifiers
     misc_feature    1..522
                     /label=RS1 recombination site
     misc_feature    1..522
                     /label=BBa_K743000
     promoter        531..680
                     /label=Pta
     promoter        531..680
                     /label=BBa_K743003
     misc_feature    687..689
                     /label=start
     CDS             687..1406
                     /label=superfolder GFP coding region
     misc_feature    1401..1406
                     /label=stop
     CDS             687..1406
                     /label=BBa_I746916
     misc_feature    1415..1447
                     /label=WT SsrA tag AANDENYALAA
     misc_feature    1415..1447
                     /label=BBa_M0050
     stem_loop       1467..1510
                     /label=stem_loop
     misc_feature    1456..1535
                     /label=BBa_B0010
     terminator      1456..1535
                     /label=BBa_B0010
     stem_loop       1551..1570
                     /label=T7 TE
     misc_feature    1577..1577
                     /label=stop
     polyA_site      1571..1584
                     /label=polya
     misc_feature    1544..1584
                     /label=BBa_B0012
     terminator      1544..1584
                     /label=BBa_B0012
     terminator      1456..1584
                     /label=BBa_B0015
     CDS             1593..2411
                     /label=kanamycin resistance
     misc_feature    2379..2379
                     /label=G to C mutation to eliminate XhoI site
     misc_feature    2516..2559
                     /label=repeat region
     misc_feature    1593..2559
                     /label=BBa_P1007
     misc_feature    1456..2559
                     /label=BBa_S05073
     misc_feature    2568..3254
                     /label=RS2
     misc_feature    2568..3254
                     /label=BBa_K743001
ORIGIN
        1 tcccagcctc tcaaccaccg atattgggcc cctagctacc cctgatattt tggcggacat
       61 tgttgcgcaa gtagaacaaa ccattgctgc tggagcccac tgtcgttgtg gcggccaagc
      121 cctagaccaa ccgggaaatt actatcctcc caccctgctc accgacgttc cccccaacgc
      181 ccctacctac cgtcaggaat tttttggccc cgtggccctc ggatttactg tcgataattt
      241 agaggaggcg atcgccttgg ccaatgacat tccctttggg ttgggggcca gtgcatggac
      301 aactaacccg gaaaatcaac aaaaactaat ccggggcata gaagccgggg ctgtattcat
      361 taacggtatg actaaatctg acccccgcat tccctttgga ggcatcaagc gctctggctt
      421 tgggcgggaa cttggccgca tgggcatttt agaatttgtc aatgctaaaa ccgtttggat
      481 tgcttagtcc gccaccattt taaaattatc gctcttctat tctactagag aacgcatcgg
      541 cgttccctag cttaaattat cttgatgtcc aaagaaggct cgctttcggg aatcaccatt
      601 accattggag ggaaagacgt tgaacttgtt taatcgccat tatgggtaag aatctcctcg
      661 aacaactgcg ccaatttaca tactagatgc gtaaaggcga agagctgttc actggtgtcg
      721 tccctattct ggtggaactg gatggtgatg tcaacggtca taagttttcc gtgcgtggcg
      781 agggtgaagg tgacgcaact aatggtaaac tgacgctgaa gttcatctgt actactggta
      841 aactgccggt accttggccg actctggtaa cgacgctgac ttatggtgtt cagtgctttg
      901 ctcgttatcc ggaccatatg aagcagcatg acttcttcaa gtccgccatg ccggaaggct
      961 atgtgcagga acgcacgatt tcctttaagg atgacggcac gtacaaaacg cgtgcggaag
     1021 tgaaatttga aggcgatacc ctggtaaacc gcattgagct gaaaggcatt gactttaaag
     1081 aagacggcaa tatcctgggc cataagctgg aatacaattt taacagccac aatgtttaca
     1141 tcaccgccga taaacaaaaa aatggcatta aagcgaattt taaaattcgc cacaacgtgg
     1201 aggatggcag cgtgcagctg gctgatcact accagcaaaa cactccaatc ggtgatggtc
     1261 ctgttctgct gccagacaat cactatctga gcacgcaaag cgttctgtct aaagatccga
     1321 acgagaaacg cgatcatatg gttctgctgg agttcgtaac cgcagcgggc atcacgcatg
     1381 gtatggatga actgtacaaa tgatgatact agaggctgct aacgacgaaa actacgctct
     1441 ggctgcttac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1501 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1561 cgggtgggcc tttctgcgtt tatatactag agttattaga aaaactcatc gagcatcaaa
     1621 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     1681 tgtaatgaag gagaaaactc accgaggcag ttccaaagaa tggcaaggtc ctggtaacgg
     1741 tctgcgattc cgacccgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     1801 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaagagc
     1861 ttgtgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     1921 ctcgcatcaa ccaaaccgtt attcatgcgt gattgcgcct gagcaagacg aaatacacga
     1981 tcgctgttaa aaggacaatt acaaacagga atcgaatgta accggcgcag gaacacggcc
     2041 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaaggctgtt
     2101 ttcccaggaa tcgcggtggt gagtaaccac gcatcatcag gagtacggat aaaatgcttg
     2161 atggtcggga gaggcataaa ctccgtcagc cagttgagac ggaccatctc atctgtaaca
     2221 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     2281 tacaagcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     2341 tataaatcag cgtccatgtt ggagtttaag cgcggacggg agcaagacgt ttcccgttga
     2401 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     2461 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
     2521 tgttgaataa atcgaacttt tgctgagttg aaggatcagt actagagagt gtaggcggta
     2581 aagtctatgg gaacttccag tttaagggct gtcccgttaa atatggcttc gagtttttga
     2641 agcataattt tctaacgtct gtggattttt ttaagcatag cttgaccttc tgttggtcga
     2701 attctaggga ttttcatcca tattcggttc atagggcagc tcaatggtga acattgctcc
     2761 cccagtttca tggttagctg cggttaaatt tcccctgtgg gccaggacaa tttccttggc
     2821 gatcgccaaa cccaggccac tgccatggcg ttgactatcg ggatgtaggc gagcacggga
     2881 actatcaccc cgataaagtc gctcaaaaat gtagggtaaa tcccttggtt gaaatcctag
     2941 gccttgatcc ctaataatca tggtgatccc ttctgtgcct tgatgaccct gcacaaaaat
     3001 agtcccttcc ggtgggctat atttcagcgt attgtccaaa atattcatca atacctgcat
     3061 cagacgatcg ccatccccct ctaggttcaa cttggttggc ccttggtaat ccagggtgat
     3121 attttttacc acggcgatcg gggttaatcg ctcccaggta atggcgagta aatggcgtaa
     3181 attaatcggt tctggctcga ggtaaagatt gggattagcg gttatttggg ttaaatggag
     3241 ccaactttcc acca
//