##gff-version 3
##sequence-region BBa_K743018 1 3254
BBa_K743018	.	engineered_region	1456	2559	.	+	0	ID=BBa_S05073;Name=BBa_S05073
BBa_K743018	.	engineered_region	1593	2559	.	+	0	ID=BBa_P1007;Name=BBa_P1007;Parent=BBa_S05073
BBa_K743018	.	sequence_feature	2516	2559	.	+	0	ID=annotation1919401;Name=repeat region;Parent=BBa_P1007
BBa_K743018	.	CDS	1593	2411	.	+	0	ID=annotation1919399;Name=kanamycin resistance;Parent=BBa_P1007
BBa_K743018	.	point_mutation	2379	2379	.	+	0	ID=annotation1919400;Name=G to C mutation to eliminate XhoI site;Parent=BBa_P1007
BBa_K743018	.	terminator	1456	1584	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S05073
BBa_K743018	.	terminator	1456	1535	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K743018	.	stem_loop	1467	1510	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K743018	.	engineered_region	1456	1535	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K743018	.	terminator	1544	1584	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K743018	.	stem_loop	1551	1570	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K743018	.	stop_codon	1577	1577	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K743018	.	polyA_site	1571	1584	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K743018	.	engineered_region	1544	1584	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K743018	.	CDS	687	1406	.	+	0	ID=BBa_I746916;Name=BBa_I746916
BBa_K743018	.	start_codon	687	689	.	+	0	ID=annotation1977533;Name=start;Parent=BBa_I746916
BBa_K743018	.	stop_codon	1401	1406	.	+	0	ID=annotation1977535;Name=stop;Parent=BBa_I746916
BBa_K743018	.	CDS	687	1406	.	+	0	ID=annotation1977534;Name=superfolder GFP coding region;Parent=BBa_I746916
BBa_K743018	.	sequence_feature	2568	3254	.	+	0	ID=BBa_K743001;Name=BBa_K743001
BBa_K743018	.	sequence_feature	2568	3254	.	+	0	ID=annotation2188524;Name=RS2;Parent=BBa_K743001
BBa_K743018	.	promoter	531	680	.	+	0	ID=BBa_K743003;Name=BBa_K743003
BBa_K743018	.	promoter	531	680	.	+	0	ID=annotation2188682;Name=Pta;Parent=BBa_K743003
BBa_K743018	.	sequence_feature	1	522	.	+	0	ID=BBa_K743000;Name=BBa_K743000
BBa_K743018	.	sequence_feature	1	522	.	+	0	ID=annotation2188518;Name=RS1 recombination site;Parent=BBa_K743000
BBa_K743018	.	tag	1415	1447	.	+	0	ID=BBa_M0050;Name=BBa_M0050
BBa_K743018	.	tag	1415	1447	.	+	0	ID=annotation1958880;Name=WT SsrA tag AANDENYALAA;Parent=BBa_M0050
>BBa_K743018
tcccagcctctcaaccaccgatattgggcccctagctacccctgatattttggcggacattgttgcgcaagtagaacaa
accattgctgctggagcccactgtcgttgtggcggccaagccctagaccaaccgggaaattactatcctcccaccctgc
tcaccgacgttccccccaacgcccctacctaccgtcaggaattttttggccccgtggccctcggatttactgtcgataa
tttagaggaggcgatcgccttggccaatgacattccctttgggttgggggccagtgcatggacaactaacccggaaaat
caacaaaaactaatccggggcatagaagccggggctgtattcattaacggtatgactaaatctgacccccgcattccct
ttggaggcatcaagcgctctggctttgggcgggaacttggccgcatgggcattttagaatttgtcaatgctaaaaccgt
ttggattgcttagtccgccaccattttaaaattatcgctcttctattctactagagaacgcatcggcgttccctagctt
aaattatcttgatgtccaaagaaggctcgctttcgggaatcaccattaccattggagggaaagacgttgaacttgttta
atcgccattatgggtaagaatctcctcgaacaactgcgccaatttacatactagatgcgtaaaggcgaagagctgttca
ctggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaagg
tgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggta
acgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgcca
tgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatt
tgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataag
ctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaa
ttcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgt
tctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggtt
ctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgatactagaggctgctaa
cgacgaaaactacgctctggctgcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tatatactagagttattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaatacca
tatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccaaagaatggcaaggtcctggtaac
ggtctgcgattccgacccgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaa
atcaccatgagtgacgactgaatccggtgagaatggcaagagcttgtgcatttctttccagacttgttcaacaggccag
ccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcatgcgtgattgcgcctgagcaagacgaaata
cacgatcgctgttaaaaggacaattacaaacaggaatcgaatgtaaccggcgcaggaacacggccagcgcatcaacaat
attttcacctgaatcaggatattcttctaatacctggaaggctgttttcccaggaatcgcggtggtgagtaaccacgca
tcatcaggagtacggataaaatgcttgatggtcgggagaggcataaactccgtcagccagttgagacggaccatctcat
ctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaagcgata
gattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcgtccatgttggagtttaag
cgcggacgggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagtt
ttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaat
aaatcgaacttttgctgagttgaaggatcagtactagagagtgtaggcggtaaagtctatgggaacttccagtttaagg
gctgtcccgttaaatatggcttcgagtttttgaagcataattttctaacgtctgtggatttttttaagcatagcttgac
cttctgttggtcgaattctagggattttcatccatattcggttcatagggcagctcaatggtgaacattgctcccccag
tttcatggttagctgcggttaaatttcccctgtgggccaggacaatttccttggcgatcgccaaacccaggccactgcc
atggcgttgactatcgggatgtaggcgagcacgggaactatcaccccgataaagtcgctcaaaaatgtagggtaaatcc
cttggttgaaatcctaggccttgatccctaataatcatggtgatcccttctgtgccttgatgaccctgcacaaaaatag
tcccttccggtgggctatatttcagcgtattgtccaaaatattcatcaatacctgcatcagacgatcgccatccccctc
taggttcaacttggttggcccttggtaatccagggtgatattttttaccacggcgatcggggttaatcgctcccaggta
atggcgagtaaatggcgtaaattaatcggttctggctcgaggtaaagattgggattagcggttatttgggttaaatgga
gccaactttccacca