sfGFP.degr
type: ComponentDefinition
persistent identity: BBa K743018
display id: BBa_K743018
version: 1
was generated by: iGEM to SBOL conversion
description: sfGFP.degradation.tag under Pta promoter in psb1C3_IntKR plasmid
created: 2012-09-22T11:00:00Z
modified: 2015-05-08T01:13:09Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _994_
m user id: 0
owner id: 11584
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K743001
component: BBa_S05073
component: BBa_M0050
component: BBa_I746916
component: BBa_K743003
component: BBa_K743000
sequence annotation: BBa_K743000
sequence annotation: BBa_K743003
sequence annotation: BBa_I746916
sequence annotation: BBa_M0050
sequence annotation: BBa_S05073
sequence annotation: BBa_K743001
sequence: https://synbiohub.org/public/igem/BBa_K743018_sequence/1

BBa_K743001
type: Component
persistent identity: component2194674
display id: component2194674
version: 1
top level: sfGFP.degr
definition: RS2
access: public

BBa_S05073
type: Component
persistent identity: component2194672
display id: component2194672
version: 1
top level: sfGFP.degr
definition: BBa_S05073
access: public

BBa_M0050
type: Component
persistent identity: component2194660
display id: component2194660
version: 1
top level: sfGFP.degr
definition: LAA
access: public

BBa_I746916
type: Component
persistent identity: component2194658
display id: component2194658
version: 1
top level: sfGFP.degr
definition: BBa_I746916
access: public

BBa_K743003
type: Component
persistent identity: component2194654
display id: component2194654
version: 1
top level: sfGFP.degr
definition: BBa_K743003
access: public

BBa_K743000
type: Component
persistent identity: component2194652
display id: component2194652
version: 1
top level: sfGFP.degr
definition: RS1
access: public

BBa_K743000
type: SequenceAnnotation
persistent identity: annotation2194652
display id: annotation2194652
version: 1
top level: sfGFP.degr
location: https://synbiohub.org/public/igem/BBa_K743018/annotation2194652/range2194652/1
component: BBa_K743000

https://synbiohub.org/public/igem/BBa_K743018/annotation2194652/range2194652/1
type: Range
persistent identity: range2194652
display id: range2194652
version: 1
top level: sfGFP.degr
direction: direction/reverse
start: 1
end: 522
orientation: inline

BBa_K743003
type: SequenceAnnotation
persistent identity: annotation2194654
display id: annotation2194654
version: 1
top level: sfGFP.degr
location: https://synbiohub.org/public/igem/BBa_K743018/annotation2194654/range2194654/1
component: BBa_K743003

https://synbiohub.org/public/igem/BBa_K743018/annotation2194654/range2194654/1
type: Range
persistent identity: range2194654
display id: range2194654
version: 1
top level: sfGFP.degr
direction: direction/reverse
start: 531
end: 680
orientation: inline

BBa_I746916
type: SequenceAnnotation
persistent identity: annotation2194658
display id: annotation2194658
version: 1
top level: sfGFP.degr
location: https://synbiohub.org/public/igem/BBa_K743018/annotation2194658/range2194658/1
component: BBa_I746916

https://synbiohub.org/public/igem/BBa_K743018/annotation2194658/range2194658/1
type: Range
persistent identity: range2194658
display id: range2194658
version: 1
top level: sfGFP.degr
direction: direction/reverse
start: 687
end: 1406
orientation: inline

BBa_M0050
type: SequenceAnnotation
persistent identity: annotation2194660
display id: annotation2194660
version: 1
top level: sfGFP.degr
location: https://synbiohub.org/public/igem/BBa_K743018/annotation2194660/range2194660/1
component: BBa_M0050

https://synbiohub.org/public/igem/BBa_K743018/annotation2194660/range2194660/1
type: Range
persistent identity: range2194660
display id: range2194660
version: 1
top level: sfGFP.degr
direction: direction/reverse
start: 1415
end: 1447
orientation: inline

BBa_S05073
type: SequenceAnnotation
persistent identity: annotation2194672
display id: annotation2194672
version: 1
top level: sfGFP.degr
location: https://synbiohub.org/public/igem/BBa_K743018/annotation2194672/range2194672/1
component: BBa_S05073

https://synbiohub.org/public/igem/BBa_K743018/annotation2194672/range2194672/1
type: Range
persistent identity: range2194672
display id: range2194672
version: 1
top level: sfGFP.degr
direction: direction/reverse
start: 1456
end: 2559
orientation: inline

BBa_K743001
type: SequenceAnnotation
persistent identity: annotation2194674
display id: annotation2194674
version: 1
top level: sfGFP.degr
location: https://synbiohub.org/public/igem/BBa_K743018/annotation2194674/range2194674/1
component: BBa_K743001

https://synbiohub.org/public/igem/BBa_K743018/annotation2194674/range2194674/1
type: Range
persistent identity: range2194674
display id: range2194674
version: 1
top level: sfGFP.degr
direction: direction/reverse
start: 2568
end: 3254
orientation: inline

LAA
type: ComponentDefinition
persistent identity: BBa M0050
display id: BBa_M0050
version: 1
was generated by: iGEM to SBOL conversion
description: AANDENYALAA. (Very fast) SsrA degradation tag.
created: 2007-12-05T12:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: C-terminal degradation tags are commonly found in high turnover proteins in Escherichia coli.
owned by: james
owned by: myers
mutable description: This sequence codes for the amino acid sequence AANDENYALAA, which when fused to the C-terminal of proteins, will make the protein susceptible to very fast degradation through SspB-mediated binding to the ClpX protease. The following rates of degradation of this tag are pulled from the corresponding references below: ~5 Vmax/ [Clpx6] min-1 from (1) ~0.5%/min on log scale from (2) ~1 min half life from (3) See the following references for further information on degradation rates and mechanisms of this tag: (1)McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701. (2)Flynn et al 2003. Mol. Cell. 11: 671. Flynn et al. 2001. PNAS 98(19): 10584. Anderson et al 1998. App. Env. Microbiol. 64(6):2240.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2398
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: C-terminal tag. Degradation rate is very fast. Deviations from this sequence in key amino acids will lower degradation rates (see Parts BBa_M0051, BBa_M0052, BBa_M0053). Three C-terminal aa's (LAA in this case) are necessary and sufficient for ClpX binding and degradation. Upstream aa sequence serves as a binding site for SspB, which guides rapid binding to ClpX.
star: false
creator: Felix Moser
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: WT SsrA tag AANDENYALAA
sequence: https://synbiohub.org/public/igem/BBa_M0050_sequence/1

WT SsrA tag AANDENYALAA
type: SequenceAnnotation
persistent identity: annotation1958880
display id: annotation1958880
version: 1
top level: LAA
location: https://synbiohub.org/public/igem/BBa_M0050/annotation1958880/range1958880/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_M0050/annotation1958880/range1958880/1
type: Range
persistent identity: range1958880
display id: range1958880
version: 1
top level: LAA
direction: direction/forward
start: 1
end: 33
orientation: inline

BBa_I746916
type: ComponentDefinition
persistent identity: BBa I746916
display id: BBa_I746916
version: 1
was generated by: iGEM to SBOL conversion
description: superfolder GFP coding sequence
created: 2008-09-29T11:00:00Z
modified: 2015-08-31T04:08:05Z
mutable provenance: Superfolder GFP was originally described by: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006 This version was synthesised de novo (by Geneart).
owned by: james
owned by: myers
mutable description: This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). It carries the following amino acid changes with respect to mut3 GFP (E0040), the currently most commonly used GFP in the registry: S30R, Y39N, F64L, G65T, F99S, N105T, Y145F, M153T, V163A, I171V, A206V Its in-vivo properties are considerably improved with respect to mut3 - it develops fluorescence about 3fold faster than mut3 GFP and reaches 4fold higher absolute fluorescence levels. Fluorescenct colonies can be identified with the naked eye even without UV or blue light illumination (that is to say the amount of blue light in normal daylight or lablight is sufficient). Additionally it is more stable in vitro and refolds faster after in vitro denaturation with respect to mut3 GFP. Note: Superfolder GFP is available in constructs driven by the pBAD and T7 promoters: part numbers I746908 and I746909 respectively. Additionally 6-his tagged versions for protein purification exist: I746914 (pBAD driven) and I746915 (T7 driven).
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Codon optimisation before de novo synthesis was carried out for both, E.coli and Bacillus subtilis.
star: false
creator: Stefan Milde
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: superfolder GFP coding region
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I746916_sequence/1

superfolder GFP coding region
type: SequenceAnnotation
persistent identity: annotation1977534
display id: annotation1977534
version: 1
top level: BBa_I746916
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977534/range1977534/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I746916/annotation1977534/range1977534/1
type: Range
persistent identity: range1977534
display id: range1977534
version: 1
top level: BBa_I746916
direction: direction/forward
start: 1
end: 720
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1977533
display id: annotation1977533
version: 1
top level: BBa_I746916
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977533/range1977533/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_I746916/annotation1977533/range1977533/1
type: Range
persistent identity: range1977533
display id: range1977533
version: 1
top level: BBa_I746916
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1977535
display id: annotation1977535
version: 1
top level: BBa_I746916
location: https://synbiohub.org/public/igem/BBa_I746916/annotation1977535/range1977535/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I746916/annotation1977535/range1977535/1
type: Range
persistent identity: range1977535
display id: range1977535
version: 1
top level: BBa_I746916
direction: direction/forward
start: 715
end: 720
orientation: inline

RS1
type: ComponentDefinition
persistent identity: BBa K743000
display id: BBa_K743000
version: 1
was generated by: iGEM to SBOL conversion
description: Synechocystis PCC6803 neutral recombination site.
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:09Z
mutable provenance: PCR amplified from synechocystis PCC6803 chromosome.
owned by: james
owned by: myers
mutable description: Any DNA sequence flacked by this part and BBa_K743001 will be incorporated into Synechocistys PCC6803 chromosome through a neutral double homologous recombination.
discontinued: false
dominant: false
experience: experience/Works
group u list: _994_
m user id: 0
owner id: 11584
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: According to literature, in Synechocystis the recombination sites must be at least 450bp long to allow a rasonably high transformation efficiency, being 700bp the optimum size.
star: false
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RS1 recombination site
sequence: https://synbiohub.org/public/igem/BBa_K743000_sequence/1

RS1 recombination site
type: SequenceAnnotation
persistent identity: annotation2188518
display id: annotation2188518
version: 1
top level: RS1
location: https://synbiohub.org/public/igem/BBa_K743000/annotation2188518/range2188518/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K743000/annotation2188518/range2188518/1
type: Range
persistent identity: range2188518
display id: range2188518
version: 1
top level: RS1
direction: direction/forward
start: 1
end: 522
orientation: inline

RS2
type: ComponentDefinition
persistent identity: BBa K743001
display id: BBa_K743001
version: 1
was generated by: iGEM to SBOL conversion
description: Synechocystis PCC6803 neutral recombination site.
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:09Z
mutable provenance: PCR amplified from Synechocystis PCC6803 chromosome, orf slr0337
owned by: james
owned by: myers
mutable description: When placed between this part and BBa_K743000, any DNA sequence will be inserted into Synechocystis PCC6803 chromosome trough homologous recombination
discontinued: false
dominant: false
experience: experience/None
group u list: _994_
m user id: 0
owner id: 11584
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: According to literature, in Synechocystis the recombination sites must be at least 450 bp long to allow a rasonably high transformation efficiency, being approximately 1200 bp the optimum size.
star: false
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: RS2
sequence: https://synbiohub.org/public/igem/BBa_K743001_sequence/1

RS2
type: SequenceAnnotation
persistent identity: annotation2188524
display id: annotation2188524
version: 1
top level: RS2
location: https://synbiohub.org/public/igem/BBa_K743001/annotation2188524/range2188524/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K743001/annotation2188524/range2188524/1
type: Range
persistent identity: range2188524
display id: range2188524
version: 1
top level: RS2
direction: direction/forward
start: 1
end: 687
orientation: inline

BBa_K743003
type: ComponentDefinition
persistent identity: BBa K743003
display id: BBa_K743003
version: 1
was generated by: iGEM to SBOL conversion
description: Pta promoter and native rbs from Synechocystis PCC6803
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:13:09Z
mutable provenance: This part was PCR amplified from Synechocystis chromosome, orf slr1739.
owned by: james
owned by: myers
mutable description: This is the 150bp sequence upstream the Transaldolase gene start codon, it contains putative rbs and promoter regions. Transaldolase is an enzyme involved in the pentose phospate cycle and its mRNA levels have shown to oscillate in a circadian manner, peaking at hour 14.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _994_
m user id: 0
owner id: 11584
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It was assumed that the 150bp sequence upstream from the start codon includes the promoter and the ribosome binding site
star: false
creator: Juan Sim??n ??lamos
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Pta
sequence: https://synbiohub.org/public/igem/BBa_K743003_sequence/1

Pta
type: SequenceAnnotation
persistent identity: annotation2188682
display id: annotation2188682
version: 1
top level: BBa_K743003
location: https://synbiohub.org/public/igem/BBa_K743003/annotation2188682/range2188682/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K743003/annotation2188682/range2188682/1
type: Range
persistent identity: range2188682
display id: range2188682
version: 1
top level: BBa_K743003
direction: direction/forward
start: 1
end: 150
orientation: inline

BBa_S05073
type: ComponentDefinition
persistent identity: BBa S05073
display id: BBa_S05073
version: 1
was generated by: iGEM to SBOL conversion
description: B0015:P1007
created: 2012-09-19T11:00:00Z
modified: 2015-05-08T01:14:49Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 11584
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Juan Sim??n ??lamos
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_P1007
component: BBa_B0015
sequence annotation: BBa_B0015
sequence annotation: BBa_P1007
sequence: https://synbiohub.org/public/igem/BBa_S05073_sequence/1

BBa_P1007
type: Component
persistent identity: component2188873
display id: component2188873
version: 1
top level: BBa_S05073
definition: KanR
access: public

BBa_B0015
type: Component
persistent identity: component2188869
display id: component2188869
version: 1
top level: BBa_S05073
definition: BBa_B0015
access: public

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2188869
display id: annotation2188869
version: 1
top level: BBa_S05073
location: https://synbiohub.org/public/igem/BBa_S05073/annotation2188869/range2188869/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_S05073/annotation2188869/range2188869/1
type: Range
persistent identity: range2188869
display id: range2188869
version: 1
top level: BBa_S05073
direction: direction/reverse
start: 1
end: 129
orientation: inline

BBa_P1007
type: SequenceAnnotation
persistent identity: annotation2188873
display id: annotation2188873
version: 1
top level: BBa_S05073
location: https://synbiohub.org/public/igem/BBa_S05073/annotation2188873/range2188873/1
component: BBa_P1007

https://synbiohub.org/public/igem/BBa_S05073/annotation2188873/range2188873/1
type: Range
persistent identity: range2188873
display id: range2188873
version: 1
top level: BBa_S05073
direction: direction/reverse
start: 138
end: 1104
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

KanR
type: ComponentDefinition
persistent identity: BBa P1007
display id: BBa_P1007
version: 1
was generated by: iGEM to SBOL conversion
description: kanamycin resistance cassette
created: 2006-02-03T12:00:00Z
modified: 2015-05-08T01:14:11Z
owned by: james
owned by: myers
mutable description: This contains KanR, a terminator and then AmpR and a terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: kanamycin resistance
sequence annotation: G to C mutation to eliminate XhoI site
sequence annotation: repeat region
sequence: https://synbiohub.org/public/igem/BBa_P1007_sequence/1

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation1919399
display id: annotation1919399
version: 1
top level: KanR
location: https://synbiohub.org/public/igem/BBa_P1007/annotation1919399/range1919399/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_P1007/annotation1919399/range1919399/1
type: Range
persistent identity: range1919399
display id: range1919399
version: 1
top level: KanR
direction: direction/reverse
start: 1
end: 819
orientation: inline

G to C mutation to eliminate XhoI site
type: SequenceAnnotation
persistent identity: annotation1919400
display id: annotation1919400
version: 1
top level: KanR
location: https://synbiohub.org/public/igem/BBa_P1007/annotation1919400/range1919400/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_P1007/annotation1919400/range1919400/1
type: Range
persistent identity: range1919400
display id: range1919400
version: 1
top level: KanR
direction: direction/forward
start: 787
end: 787
orientation: inline

repeat region
type: SequenceAnnotation
persistent identity: annotation1919401
display id: annotation1919401
version: 1
top level: KanR
location: https://synbiohub.org/public/igem/BBa_P1007/annotation1919401/range1919401/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_P1007/annotation1919401/range1919401/1
type: Range
persistent identity: range1919401
display id: range1919401
version: 1
top level: KanR
direction: direction/reverse
start: 924
end: 967
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K743018_sequence/1
type: Sequence
persistent identity: BBa K743018 sequence
display id: BBa_K743018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccagcctctcaaccaccgatattgggcccctagctacccctgatattttggcggacattgttgcgcaagtagaacaaaccattgctgctggagcccactgtcgttgtggcggccaagccctagaccaaccgggaaattactatcctcccaccctgctcaccgacgttccccccaacgcccctacctaccgtcaggaattttttggccccgtggccctcggatttactgtcgataatttagaggaggcgatcgccttggccaatgacattccctttgggttgggggccagtgcatggacaactaacccggaaaatcaacaaaaactaatccggggcatagaagccggggctgtattcattaacggtatgactaaatctgacccccgcattccctttggaggcatcaagcgctctggctttgggcgggaacttggccgcatgggcattttagaatttgtcaatgctaaaaccgtttggattgcttagtccgccaccattttaaaattatcgctcttctattctactagagaacgcatcggcgttccctagcttaaattatcttgatgtccaaagaaggctcgctttcgggaatcaccattaccattggagggaaagacgttgaacttgtttaatcgccattatgggtaagaatctcctcgaacaactgcgccaatttacatactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgatactagaggctgctaacgacgaaaactacgctctggctgcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccaaagaatggcaaggtcctggtaacggtctgcgattccgacccgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaagagcttgtgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcatgcgtgattgcgcctgagcaagacgaaatacacgatcgctgttaaaaggacaatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M0050_sequence/1
type: Sequence
persistent identity: BBa M0050 sequence
display id: BBa_M0050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgctaacgacgaaaactacgctctggctgct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746916_sequence/1
type: Sequence
persistent identity: BBa I746916 sequence
display id: BBa_I746916_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K743000_sequence/1
type: Sequence
persistent identity: BBa K743000 sequence
display id: BBa_K743000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccagcctctcaaccaccgatattgggcccctagctacccctgatattttggcggacattgttgcgcaagtagaacaaaccattgctgctggagcccactgtcgttgtggcggccaagccctagaccaaccgggaaattactatcctcccaccctgctcaccgacgttccccccaacgcccctacctaccgtcaggaattttttggccccgtggccctcggatttactgtcgataatttagaggaggcgatcgccttggccaatgacattccctttgggttgggggccagtgcatggacaactaacccggaaaatcaacaaaaactaatccggggcatagaagccggggctgtattcattaacggtatgactaaatctgacccccgcattccctttggaggcatcaagcgctctggctttgggcgggaacttggccgcatgggcattttagaatttgtcaatgctaaaaccgtttggattgcttagtccgccaccattttaaaattatcgctcttctattc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K743001_sequence/1
type: Sequence
persistent identity: BBa K743001 sequence
display id: BBa_K743001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtgtaggcggtaaagtctatgggaacttccagtttaagggctgtcccgttaaatatggcttcgagtttttgaagcataattttctaacgtctgtggatttttttaagcatagcttgaccttctgttggtcgaattctagggattttcatccatattcggttcatagggcagctcaatggtgaacattgctcccccagtttcatggttagctgcggttaaatttcccctgtgggccaggacaatttccttggcgatcgccaaacccaggccactgccatggcgttgactatcgggatgtaggcgagcacgggaactatcaccccgataaagtcgctcaaaaatgtagggtaaatcccttggttgaaatcctaggccttgatccctaataatcatggtgatcccttctgtgccttgatgaccctgcacaaaaatagtcccttccggtgggctatatttcagcgtattgtccaaaatattcatcaatacctgcatcagacgatcgccatccccctctaggttcaacttggttggcccttggtaatccagggtgatattttttaccacggcgatcggggttaatcgctcccaggtaatggcgagtaaatggcgtaaattaatcggttctggctcgaggtaaagattgggattagcggttatttgggttaaatggagccaactttccacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K743003_sequence/1
type: Sequence
persistent identity: BBa K743003 sequence
display id: BBa_K743003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacgcatcggcgttccctagcttaaattatcttgatgtccaaagaaggctcgctttcgggaatcaccattaccattggagggaaagacgttgaacttgtttaatcgccattatgggtaagaatctcctcgaacaactgcgccaatttaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05073_sequence/1
type: Sequence
persistent identity: BBa S05073 sequence
display id: BBa_S05073_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccaaagaatggcaaggtcctggtaacggtctgcgattccgacccgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaagagcttgtgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcatgcgtgattgcgcctgagcaagacgaaatacacgatcgctgttaaaaggacaattacaaacaggaatcgaatgtaaccggcgcaggaacacggccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaaggctgttttcccaggaatcgcggtggtgagtaaccacgcatcatcaggagtacggataaaatgcttgatggtcgggagaggcataaactccgtcagccagttgagacggaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaagcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcgtccatgttggagtttaagcgcggacgggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1007_sequence/1
type: Sequence
persistent identity: BBa P1007 sequence
display id: BBa_P1007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccaaagaatggcaaggtcctggtaacggtctgcgattccgacccgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaagagcttgtgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcatgcgtgattgcgcctgagcaagacgaaatacacgatcgctgttaaaaggacaattacaaacaggaatcgaatgtaaccggcgcaggaacacggccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaaggctgttttcccaggaatcgcggtggtgagtaaccacgcatcatcaggagtacggataaaatgcttgatggtcgggagaggcataaactccgtcagccagttgagacggaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaagcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcgtccatgttggagtttaagcgcggacgggagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatatttttatcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaaggatcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z