LOCUS       BBa_K747097             2688 bp    DNA     linear       17-May-2024
DEFINITION  Prokaryotic_expression_plasmid
ACCESSION   BBa_K747097
VERSION     BBa_K747097.1  
FEATURES             Location/Qualifiers
     promoter        137..166
                     /label=lac
     misc_feature    171..193
                     /label=LacO
     RBS             228..239
                     /label=RBS
     CDS             303..326
                     /label=Flag tag
     misc_feature    333..356
                     /label=SV40 NLS1
     RBS             906..1562
                     /label=CmR
     CDS             2000..2208
                     /label=ccdB
     misc_feature    2506..2552
                     /label=PPEC
     misc_feature    2560..2688
                     /label=Terminator
     CDS             243..260
                     /label=6His
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa actagagagg aggacagcta
      241 tgcatcatca ccatcaccac gactataagg accacgacgg agactacaag gatcatgata
      301 ttgattacaa agacgatgac gataagatgg ccccaaagaa gaagcggaag gtcggtatcc
      361 acggagtccc agcagccgta gatttgagaa ctttgggata ttcacagcag cagcaggaaa
      421 agatcaagcc caaagtgagg tcgacagtcg cgcagcatca cgaagcgctg gtgggtcatg
      481 ggtttacaca tgcccacatc gtagccttgt cgcagcaccc tgccgccctt ggcacggtcg
      541 ccgtcaagta ccaggacatg attgcggcgt tgccggaagc cacacatgag gcgatcgtcg
      601 gtgtggggaa acagtggagc ggagcccgag cgcttgaggc cctgttgacg gtcgcgggag
      661 agctgagagg gcctcccctt cagctggaca cgggccagtt gctgaagatc gcgaagcggg
      721 gaggagtcac ggcggtcgag gcggtgcacg cgtggcgcaa tgcgctcacg ggagcacccc
      781 tcaacctgac agagacggcg gccgcattag gcaccccagg ctttacactt tatgcttccg
      841 gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag
      901 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
      961 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     1021 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     1081 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga gttccgtatg gcaatgaaag
     1141 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     1201 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     1261 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     1321 ttgagaatat gtttttcgtg tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     1381 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     1441 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     1501 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     1561 cgtaaagatc tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg
     1621 atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta
     1681 tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc
     1741 atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt
     1801 ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt
     1861 attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt
     1921 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
     1981 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
     2041 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
     2101 caccgatatg gccagtgtgc ccgtctccgt tatcggggaa gaagtggctg atctcagcca
     2161 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
     2221 ctcccttata cacagccagt ctgccggtcg accgtctcga ctcacgcctg agcaggtagt
     2281 ggctattgca tccaacggag ggggcagacc cgcactggag tcaatcgtgg cccagctttc
     2341 gaggccggac cccgcgctgg ccgcactcac taatgatcat cttgtagcgc tggcctgcct
     2401 cggcggacga cccgccttgg atgcggtgaa gaaggggctc ccgcacgcgc ctgcattgat
     2461 taagcggacc aacagaagga ttcccgagag gacatcacat cgagtggcaa tgtcttcaac
     2521 atcggtttaa acgccactta gaagacgata aatactagac caggcatcaa ataaaacgaa
     2581 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     2641 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//