LOCUS BBa_K747099 6416 bp DNA linear 17-May-2024 DEFINITION Eukaryotic TAL expression plasmid ACCESSION BBa_K747099 VERSION BBa_K747099.1 FEATURES Location/Qualifiers misc_feature 2..805 /label=pUC Ori misc_feature 1588..3900 /label=TALE casette CDS 1591..1608 /label=6His CDS 1651..1674 /label=Flag tag misc_feature 1681..1704 /label=SV40 NLS1 misc_feature 3854..3900 /label=PPEC misc_feature 4350..4427 /label=SV40 ORI CDS 5381..6235 /label=AmpR promoter 6280..6308 /label=Amp prom misc_feature 988..1546 /label=CMV promoter 3922..4547 /label=SV40 CDS 4552..5147 /label=PuroORF misc_feature 5202..5332 /label=SV 40 pA misc_feature 3348..3556 /label=ccdB CDS 2254..2910 /label=CmR ORIGIN 1 ctcatgacca aaatccctta acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc 61 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 121 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 181 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 241 tgtagccgta gttagcccac cacttcaaga actctgtagc accgcctaca tacctcgctc 301 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 361 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 421 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 481 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 541 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 601 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 661 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 721 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 781 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 841 gcgaggaagc ggaaggcgag agtagggaac tgccaggcat caaactaagc agaaggcccc 901 tgacggatgg cctttttgcg tttctacaaa ctctttctgt gttgtaaaac gacggccagt 961 cttaagaatt cgcggccgct tctagagata gtaatcaatt acggggtcat tagttcatag 1021 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 1081 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 1141 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 1201 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 1261 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 1321 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 1381 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 1441 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 1501 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg 1561 tcagatctac tagagaggag gacagctatg catcatcacc atcaccacga ctataaggac 1621 cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc 1681 ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgtaga tttgagaact 1741 ttgggatatt cacagcagca gcaggaaaag atcaagccca aagtgaggtc gacagtcgcg 1801 cagcatcacg aagcgctggt gggtcatggg tttacacatg cccacatcgt agccttgtcg 1861 cagcaccctg ccgcccttgg cacggtcgcc gtcaagtacc aggacatgat tgcggcgttg 1921 ccggaagcca cacatgaggc gatcgtcggt gtggggaaac agtggagcgg agcccgagcg 1981 cttgaggccc tgttgacggt cgcgggagag ctgagagggc ctccccttca gctggacacg 2041 ggccagttgc tgaagatcgc gaagcgggga ggagtcacgg cggtcgaggc ggtgcacgcg 2101 tggcgcaatg cgctcacggg agcacccctc aacctgacag agacggcggc cgcattaggc 2161 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat 2221 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc 2281 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct 2341 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag 2401 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct 2461 catccggagt tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac 2521 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac 2581 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa 2641 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtgtc agccaatccc 2701 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc 2761 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt 2821 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa 2881 cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc 2941 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg 3001 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg 3061 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag 3121 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca 3181 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa 3241 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc 3301 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc 3361 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata 3421 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccc gtctccgtta 3481 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc 3541 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gccggtcgac 3601 cgtctcgact cacgcctgag caggtagtgg ctattgcatc caacggaggg ggcagacccg 3661 cactggagtc aatcgtggcc cagctttcga ggccggaccc cgcgctggcc gcactcacta 3721 atgatcatct tgtagcgctg gcctgcctcg gcggacgacc cgccttggat gcggtgaaga 3781 aggggctccc gcacgcgcct gcattgatta agcggaccaa cagaaggatt cccgagagga 3841 catcacatcg agtggcaatg tcttcaacat cggtttaaac gccacttaga agacgataaa 3901 tactagtagc ggccgctgca gaaaatcagc ctcgactgtg ccttctagtt gccagccatc 3961 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 4021 ttcctaataa aatgaggaaa ttgcatcaca acactcaacc ctatctcggt ctattctttt 4081 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa 4141 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 4201 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 4261 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 4321 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 4381 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg 4441 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 4501 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgatg accgagtaca 4561 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg 4621 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc 4681 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt 4741 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg 4801 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg 4861 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc 4921 tggccaccgt cggcgtgtcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc 4981 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggaa acctccgcgc 5041 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgagtgcc 5101 cgaaggaccg cgcgacctgg tgcatgaccc gcaagcccgg tgcctgaccg gctggatgat 5161 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc 5221 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 5281 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc 5341 gtcgacctct agctagagct tggcgtaatc atggtcatta ccaatgctta atcagtgagg 5401 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 5461 agataactac gatacgggag ggcttaccat ctggccccag cgctgcgatg ataccgcgag 5521 aaccacgctc accggctccg gatttatcag caataaacca gccagccgga agggccgagc 5581 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 5641 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatc gctacaggca 5701 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 5761 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 5821 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 5881 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 5941 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 6001 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 6061 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 6121 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 6181 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatat 6241 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 6301 tatttgaatg tatttagaaa aataaacaaa taggggtcag tgttacaacc aattaaccaa 6361 ttctgaacat tatcgcgagc ccatttatac ctgaatatgg ctcataacac cccttg //