LOCUS BBa_K747100 6977 bp DNA linear 25-Nov-2024 DEFINITION pTALEN ACCESSION BBa_K747100 VERSION BBa_K747100.1 FEATURES Location/Qualifiers misc_feature 2..805 /label=pUC Ori CDS 1591..1608 /label=6His CDS 1651..1674 /label=Flag tag misc_feature 1681..1704 /label=SV40 NLS1 misc_feature 4911..4988 /label=SV40 Ori misc_feature 5113..5708 /label=PuroORF misc_feature 5763..5893 /label=SV40 pA CDS 5948..6796 /label=AmpR CDS 3854..4461 /label=Fok1 misc_feature 4468..5112 /label=PostORF promoter 988..1546 /label=CMV CDS 3348..3556 /label=ccdB CDS 2254..2910 /label=CmR ORIGIN 1 ctcatgacca aaatccctta acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc 61 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 121 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 181 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 241 tgtagccgta gttagcccac cacttcaaga actctgtagc accgcctaca tacctcgctc 301 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 361 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 421 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 481 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 541 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 601 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 661 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 721 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 781 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 841 gcgaggaagc ggaaggcgag agtagggaac tgccaggcat caaactaagc agaaggcccc 901 tgacggatgg cctttttgcg tttctacaaa ctctttctgt gttgtaaaac gacggccagt 961 cttaagaatt cgcggccgct tctagagata gtaatcaatt acggggtcat tagttcatag 1021 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 1081 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 1141 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 1201 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 1261 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 1321 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 1381 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 1441 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 1501 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg 1561 tcagatctac tagagaggag gacagctatg catcatcacc atcaccacga ctataaggac 1621 cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc 1681 ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgtaga tttgagaact 1741 ttgggatatt cacagcagca gcaggaaaag atcaagccca aagtgaggtc gacagtcgcg 1801 cagcatcacg aagcgctggt gggtcatggg tttacacatg cccacatcgt agccttgtcg 1861 cagcaccctg ccgcccttgg cacggtcgcc gtcaagtacc aggacatgat tgcggcgttg 1921 ccggaagcca cacatgaggc gatcgtcggt gtggggaaac agtggagcgg agcccgagcg 1981 cttgaggccc tgttgacggt cgcgggagag ctgagagggc ctccccttca gctggacacg 2041 ggccagttgc tgaagatcgc gaagcgggga ggagtcacgg cggtcgaggc ggtgcacgcg 2101 tggcgcaatg cgctcacggg agcacccctc aacctgacag agacggcggc cgcattaggc 2161 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat 2221 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc 2281 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct 2341 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag 2401 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct 2461 catccggagt tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac 2521 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac 2581 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa 2641 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtgtc agccaatccc 2701 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc 2761 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt 2821 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa 2881 cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt actaaaagcc 2941 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg 3001 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg 3061 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag 3121 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca 3181 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa 3241 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc 3301 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc 3361 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata 3421 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccc gtctccgtta 3481 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc 3541 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gccggtcgac 3601 cgtctcgact cacgcctgag caggtagtgg ctattgcatc caacggaggg ggcagacccg 3661 cactggagtc aatcgtggcc cagctttcga ggccggaccc cgcgctggcc gcactcacta 3721 atgatcatct tgtagcgctg gcctgcctcg gcggacgacc cgccttggat gcggtgaaga 3781 aggggctccc gcacgcgcct gcattgatta agcggaccaa cagaaggatt cccgagagga 3841 catcacatcg agtggcaggt tcccaactcg tgaagagtga acttgaggag aaaaagtcgg 3901 agctgcggca caaattgaaa tacgtaccgc atgaatacat cgaacttatc gaaattgcta 3961 ggaactcgac tcaagacaga atccttgaga tgaaggtaat ggagttcttt atgaaggttt 4021 atggataccg agggaagcat ctcggtggat cacgaaaacc cgacggagca atctatacgg 4081 tggggagccc gattgattac ggagtgatcg tcgacacgaa agcctacagc ggtgggtaca 4141 atcttcccat cgggcaggca gatgagatgc aacgttatgt cgaagaaaat cagaccagga 4201 acaaacacat caatccaaat gagtggtgga aagtgtatcc ttcatcagtg accgagttta 4261 agtttttgtt tgtctctggg catttcaaag gcaactataa ggcccagctc acacggttga 4321 atcacattac gaactgcaat ggtgcggttt tgtccgtaga ggaactgctc attggtggag 4381 aaatgatcaa agcgggaact ctgacactgg aagaagtcag acgcaagttt aacaatggcg 4441 agatcaattt ccgctcataa atactagtag cggccgctgc agaaaatcag cctcgactgt 4501 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga 4561 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcac aacactcaac 4621 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 4681 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt 4741 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 4801 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 4861 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc 4921 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 4981 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg 5041 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc 5101 agcacgtgat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca 5161 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg 5221 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg 5281 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca 5341 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt 5401 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc 5461 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtgtc gcccgaccac cagggcaagg 5521 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg 5581 ccttcctgga aacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg 5641 tcaccgccga cgtcgagtgc ccgaaggacc gcgcgacctg gtgcatgacc cgcaagcccg 5701 gtgcctgacc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 5761 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 5821 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 5881 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcatt 5941 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 6001 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 6061 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc 6121 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 6181 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 6241 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 6301 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 6361 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 6421 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 6481 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 6541 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 6601 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 6661 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 6721 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 6781 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt 6841 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca 6901 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg 6961 gctcataaca ccccttg //