LOCUS BBa_K747102 6582 bp DNA linear 28-Dec-2024 DEFINITION pTAL-AID ACCESSION BBa_K747102 VERSION BBa_K747102.1 FEATURES Location/Qualifiers misc_feature 5858..6486 /label=ColE1 origin misc_feature 3508..3948 /label=F1 ori CDS 4481..5272 /label=Kan/neo resistance promoter 4247..4443 /label=SV40 early promoter promoter 4015..4043 /label=Amp promoter misc_feature 4294..4371 /label=SV40 ori misc_feature 613..630 /label=6-his-tag misc_feature 673..696 /label=Flag tag misc_feature 703..726 /label=SV40 NLS1 CDS 2625..3202 /label=AID promoter 1..568 /label=CMV CDS 1061..1160 /label=Tal Repeat (T) CDS 1161..1358 /label=Tal Direpeat (GC) CDS 1365..1562 /label=Tal Direpeat (GG) CDS 1569..1766 /label=Tal Direpeat (TC) CDS 1773..1970 /label=Tal Direpeat (TA) CDS 1977..2174 /label=Tal Direpeat (TT) CDS 2181..2378 /label=Tal Direpeat (AC) CDS 2382..2481 /label=Tal Repeat (T) ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagagagg 601 aggacagcta tgcatcatca ccatcaccac gactataagg accacgacgg agactacaag 661 gatcatgata ttgattacaa agacgatgac gataagatgg ccccaaagaa gaagcggaag 721 gtcggtatcc acggagtccc agcagccgta gatttgagaa ctttgggata ttcacagcag 781 cagcaggaaa agatcaagcc caaagtgagg tcgacagtcg cgcagcatca cgaagcgctg 841 gtgggtcatg ggtttacaca tgcccacatc gtagccttgt cgcagcaccc tgccgccctt 901 ggcacggtcg ccgtcaagta ccaggacatg attgcggcgt tgccggaagc cacacatgag 961 gcgatcgtcg gtgtggggaa acagtggagc ggagcccgag cgcttgaggc cctgttgacg 1021 gtcgcgggag agctgagagg gcctcccctt cagctggaca cgggccagtt gctgaagatc 1081 gcgaagcggg gaggagtcac ggcggtcgag gcggtgcacg cgtggcgcaa tgcgctcacg 1141 ggagcacccc tcaacctgac cccggaacag gtggtggcca tcgcctctaa caatggcggc 1201 aaacaggcat tggaaacagt tcagcgcctg ctgccggtgt tgtgtcaggc tcacggcctg 1261 actccggagc aggttgtggc catcgcaagc catgatggcg gtaaacaagc tctggagaca 1321 gtgcaacgcc tcttgccagt gctgtgtcag gcccacgggc tcaccccgga acaggtggtg 1381 gccattgcct ctaataacgg tggcaaacaa gctctggaga ccgtccagcg gcttctgcca 1441 gtgctgtgtc aagcccatgg cctgactcca gagcaggtgg tcgcgatcgc ttccaacaat 1501 ggcggcaaac aggccctcga aacagtgcaa cgcctgttgc cggtgctgtg tcaggcccac 1561 ggcttgaccc cggaacaggt ggtggccatt gcaagcaacg gtggtggcaa gcaggccctg 1621 gagacagtcc aacggctgct tccggttctg tgtcaggccc acggcctgac tccagaacaa 1681 gtggttgcta tcgccagcca cgatggcggt aaacaagccc tcgaaaccgt gcagcgcctg 1741 cttccggtgc tgtgtcaggc ccacgggctt accccggaac aggtggtggc catcgcctcc 1801 aacggcggtg gcaaacaggc gctggaaaca gttcaacgcc tccttccggt cctgtgccag 1861 gcccatggtc tgactccaga gcaggttgtg gcaattgcaa gcaacattgg tggtaaacaa 1921 gctttggaaa ccgtccagcg cttgctgcca gtgctgtgtc aggcccacgg actgaccccg 1981 gaacaggtgg tggccatcgc tagcaatggc ggtggtaaac aggcgctgga gacagtgcag 2041 cgcttgcttc cggtgctgtg tcaagcacat ggtctcaccc cagaacaggt ggttgcaatt 2101 gcgagcaacg gcggcggtaa gcaggcattg gagactgtcc agcgcctgct gccggtgctg 2161 tgtcaggccc acggcctgac cccggaacag gtggtggcca tcgcctccaa catcggcggc 2221 aaacaggcgt tggagaccgt gcaacgcctg ctgccagtct tgtgccaagc ccacggcctc 2281 acaccggaac aagtggttgc aattgcctct catgacggtg gtaagcaagc actggaaact 2341 gtccagcgcc tgcttccggt gctgtgtcag gcccacggac tcacgcctga gcaggtagtg 2401 gctattgcat ccaacggagg gggcagaccc gcactggagt caatcgtggc ccagctttcg 2461 aggccggacc ccgcgctggc cgcactcact aatgatcatc ttgtagcgct ggcctgcctc 2521 ggcggacgac ccgccttgga tgcggtgaag aaggggctcc cgcacgcgcc tgcattgatt 2581 aagcggacca acagaaggat tcccgagagg acatcacatc gagtggcagg tggtggttcg 2641 ggtggtatgg acagcctctt gatgaaccgg aggaagtttc tttaccaatt caaaaatgtc 2701 cgctgggcta agggtcggcg tgagacctac ctgtgctacg tagtgaagag gcgtgacagt 2761 gctacatcct tttcactgga ctttggttat cttcgcaata agaacggctg ccacgtggaa 2821 ttgctcttcc tccgctacat ctcggactgg gacctagacc ctggccgctg ctaccgcgtc 2881 acctggttca cctcctggag cccctgctac gactgtgccc gacatgtggc cgactttctg 2941 cgagggaacc ccaacctcag tctgaggatc ttcaccgcgc gcctctactt ctgtgaggac 3001 cgcaaggctg agcccgaggg gctgcggcgg ctgcaccgcg ccggggtgca aatagccatc 3061 atgaccttca aagattattt ttactgctgg aatacttttg tagaaaacca tgaaagaact 3121 ttcaaagcct gggaagggct gcatgaaaat tcagttcgtc tctccagaca gcttcggcgc 3181 atccttttgc cctagtaaat actagtagcg gccgctgcag tcgacggtac cgcgggcccg 3241 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta 3301 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt 3361 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 3421 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 3481 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt 3541 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc 3601 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 3661 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact 3721 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg 3781 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 3841 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac 3901 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg 3961 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 4021 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 4081 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc 4141 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 4201 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 4261 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 4321 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 4381 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 4441 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 4501 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 4561 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 4621 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc 4681 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 4741 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 4801 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 4861 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 4921 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 4981 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca 5041 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 5101 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 5161 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 5221 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact 5281 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc 5341 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 5401 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact 5461 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag 5521 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg 5581 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt 5641 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg 5701 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat 5761 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 5821 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 5881 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 5941 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 6001 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 6061 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 6121 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 6181 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 6241 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 6301 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 6361 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 6421 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 6481 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 6541 ttatcccctg attctgtgga taaccgtatt accgccatgc at //