LOCUS       BBa_K748006              959 bp    DNA     linear       25-Nov-2024
DEFINITION  S.aureus killing device. Truncated lysostaphin coding sequence 
            with IPTG inducible promoter.
ACCESSION   BBa_K748006
VERSION     BBa_K748006.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..822
                     /label=BBa_K748002
     stem_loop       842..885
                     /label=stem_loop
     misc_feature    831..910
                     /label=BBa_B0010
     terminator      831..910
                     /label=BBa_B0010
     stem_loop       926..945
                     /label=T7 TE
     misc_feature    952..952
                     /label=stop
     polyA_site      946..959
                     /label=polya
     misc_feature    919..959
                     /label=BBa_B0012
     terminator      919..959
                     /label=BBa_B0012
     terminator      831..959
                     /label=BBa_B0015
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgacacat gaacattcag cacaatggtt gaataattac
      121 aaaaaaggat atggttacgg tccttatcca ttaggtataa atggcggtat gcactacgga
      181 gttgattttt ttatgaatat tggaacacca gtaaaagcta tttcaagcgg aaaaatagtt
      241 gaagctggtt ggagtaatta cggaggaggt aatcaaatag gtcttattga aaatgatgga
      301 gtgcatagac aatggtatat gcatctaagt aaatataatg ttaaagtagg agattatgtc
      361 aaagctggtc aaataatcgg ttggtctgga agcactggtt attctacagc accacattta
      421 cacttccaaa gaatggttaa ttcattttca aattcaactg cccaagatcc aatgcctttc
      481 ttaaagagcg caggatatgg aaaagcaggt ggtacagtaa ctccaacgcc gaatacaggt
      541 tggaaaacaa acaaatatgg cacactatat aaatcagagt cagctagctt cacacctaat
      601 acagatataa taacaagaac gactggtcca tttagaagca tgccgcagtc aggagtctta
      661 aaagcaggtc aaacaattca ttatgatgaa gtgatgaaac aagacggtca tgtttgggta
      721 ggttatacag gtaacagtgg ccaacgtatt tacttgcctg taagaacatg gaataaatct
      781 actaatactt taggtgttct ttggggaact ataaagtaat aatactagag ccaggcatca
      841 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      901 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//