BBa_K748003
1
BBa_K748003
yddV is a Diguanylate Cyclase-Genomic.
2012-09-14T11:00:00Z
2015-05-08T01:13:12Z
de novo synthesis
Released HQ 2013
yddV is a Diguanylate Cyclase-Genomic. The product of gene yddV has diguanylate cyclase (DGC) activity. DGC uses 2 GTP to form a Bis-(3???-5???)-cyclic dimeric guanosine monophosphate (c-di-GMP). C-di-GMP is a global second messenger in bacteria. Biofilm formation of E.coli is manipulable by varying c-di-GMP concentrations. When the c-di-GMP level stays low, there is little biofilm and the bacteria is dispersive. High concentrations of c-di-GMP promote bacteria to form more cellulose and fimbriae, which enhances the biofilm formation and decrease the motility of bacteria.
false
false
_999_
0
8564
9
In stock
false
nothing special
false
Lei Qiao
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_K748007
1
BBa_K748007
Biofilm formation device. yddV gene with the IPTG inducible promoter.
2012-09-14T11:00:00Z
2015-05-08T01:13:12Z
de novo synthesis
　　Gene yddV is under the regulation of Ptrc promoter (IPTG-inducible). yddV is a Diguanylate Cyclase-Genomic. The product of gene yddV has diguanylate cyclase (DGC) activity. DGC uses 2 GTP to form a Bis-(3???-5???)-cyclic dimeric guanosine monophosphate (c-di-GMP). C-di-GMP is a global second messenger in bacteria. Biofilm formation of E.coli is manipulable by varying c-di-GMP concentrations. When the c-di-GMP level stays low, there is little biofilm and the bacteria is dispersive. High concentrations of c-di-GMP promote bacteria to form more cellulose and fimbriae, which enhances the biofilm formation and decrease the motility of bacteria.
false
false
_999_
0
8564
9
It's complicated
true
nothing special
false
Lei Qiao
component2183401
1
BBa_B0034
component2183395
1
BBa_R0011
component2183402
1
BBa_K748003
component2183409
1
BBa_B0015
annotation2183395
1
BBa_R0011
range2183395
1
1
54
annotation2183401
1
BBa_B0034
range2183401
1
64
75
annotation2183402
1
BBa_K748003
range2183402
1
82
1467
annotation2183409
1
BBa_B0015
range2183409
1
1476
1604
BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation2001
1
lac O1
range2001
1
26
42
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation1999
1
lac O1
range1999
1
3
19
annotation2000
1
-35
range2000
1
20
25
annotation2002
1
-10
range2002
1
43
48
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K748003_sequence
1
atggaaatgtattttaaacgtatgaaagatgaatggaccggtctggttgaacaagcagatcctccaattagagctaaagctgctgagatagcggtggctcacgctcactacttgagtatagagttttacagaatcgtacgaatcgatcctcacgctgaggagttcttgagtaacgaacaggtagagagacagttgaagagtgctatggagagatggataataaatgtactttctgcccaggtagacgacgtagaaagacttatacagatacagcacactgtagctgaagttcatgctagaataggaatacctgtagaaatagtagagatgggtttcagagtgcttaaaaagatattgtaccctgtaattttcagtagtgactacagtgctgctgagaagttgcaagtatatcacttttcaataaatagtatagacatagctatggaagtaatgactagagctttcactttctctgacagtagtgcttctaaagaggatgagaattacagaatattcagtcttcttgaaaacgctgaagaggaaaaagagagacaaatagcttctatattgagttgggaaattgacataatatacaagatacttcttgattctgatttgggcagtagtttgcctttgtctcaggctgacttcggcctttggttcaaccacaagggacgacactacttcagcggaatagctgaggtaggccatatatctcgacttatacaggatttcgatggaattttcaatcagacgatgagaaatacgagaaacttgaacaaccgttcattgagagtaaaattcttgcttcagataagaaacactgtgagccagataataactcttctacgagagttgttcgaggaggttagtcgacacgaggtaggcatggatgtgcttacgaaattgctaaacagacgattcttgccgaccatcttcaagagagagatagcgcacgctaaccgaactggcacgcctctttcggtattgatcatagatgtagataagttcaaagagataaatgacacatggggacataatactggtgatgaaatattgagaaaggtaagtcaagctttttacgataacgtacgaagttctgattacgttttcagatacggaggcgacgagttcataatagtgctaacggaagcttctgagaacgagacgcttcgaacggctgaaagaatacgtagtcgagtagaaaaaactaagttgaaggctgctaacggagaggatatagctttgagtctatctataggagctgctatgtttaacggacacccagattacgagcgacttatacagatagcggatgaggcactttacatagctaagagaagaggaagaaacagagtagagttgtggaaagctagtttgtaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
BBa_K748007_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggaaatgtattttaaacgtatgaaagatgaatggaccggtctggttgaacaagcagatcctccaattagagctaaagctgctgagatagcggtggctcacgctcactacttgagtatagagttttacagaatcgtacgaatcgatcctcacgctgaggagttcttgagtaacgaacaggtagagagacagttgaagagtgctatggagagatggataataaatgtactttctgcccaggtagacgacgtagaaagacttatacagatacagcacactgtagctgaagttcatgctagaataggaatacctgtagaaatagtagagatgggtttcagagtgcttaaaaagatattgtaccctgtaattttcagtagtgactacagtgctgctgagaagttgcaagtatatcacttttcaataaatagtatagacatagctatggaagtaatgactagagctttcactttctctgacagtagtgcttctaaagaggatgagaattacagaatattcagtcttcttgaaaacgctgaagaggaaaaagagagacaaatagcttctatattgagttgggaaattgacataatatacaagatacttcttgattctgatttgggcagtagtttgcctttgtctcaggctgacttcggcctttggttcaaccacaagggacgacactacttcagcggaatagctgaggtaggccatatatctcgacttatacaggatttcgatggaattttcaatcagacgatgagaaatacgagaaacttgaacaaccgttcattgagagtaaaattcttgcttcagataagaaacactgtgagccagataataactcttctacgagagttgttcgaggaggttagtcgacacgaggtaggcatggatgtgcttacgaaattgctaaacagacgattcttgccgaccatcttcaagagagagatagcgcacgctaaccgaactggcacgcctctttcggtattgatcatagatgtagataagttcaaagagataaatgacacatggggacataatactggtgatgaaatattgagaaaggtaagtcaagctttttacgataacgtacgaagttctgattacgttttcagatacggaggcgacgagttcataatagtgctaacggaagcttctgagaacgagacgcttcgaacggctgaaagaatacgtagtcgagtagaaaaaactaagttgaaggctgctaacggagaggatatagctttgagtctatctataggagctgctatgtttaacggacacccagattacgagcgacttatacagatagcggatgaggcactttacatagctaagagaagaggaagaaacagagtagagttgtggaaagctagtttgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z