BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K749006
1
BBa_K749006
formate dehydrogenase 4 α-subunit from Methylobacterium extorquens
2012-09-25T11:00:00Z
2015-05-08T01:13:12Z
Methylobacterium extorquens
formate dehydrogenase 4 from Methylobacterium extorquens
false
false
_1000_
0
8628
9
Not in stock
false
None
false
Takuya Akutsu
BBa_K749015
1
BBa_K749015
formate dehydrogenase device
2012-09-25T11:00:00Z
2015-05-08T01:13:12Z
Methylobacterium extorquens consecutively
Formate dehydrogenase device
false
false
_1000_
0
8628
9
It's complicated
true
none
false
Takuya Akutsu
component2199485
1
BBa_B0034
component2199487
1
BBa_K749007
component2199494
1
BBa_B0015
component2199483
1
BBa_J23100
component2199486
1
BBa_K749006
annotation2199483
1
BBa_J23100
range2199483
1
1
35
annotation2199487
1
BBa_K749007
range2199487
1
2389
2712
annotation2199486
1
BBa_K749006
range2199486
1
62
2380
annotation2199494
1
BBa_B0015
range2199494
1
2721
2849
annotation2199485
1
BBa_B0034
range2199485
1
44
55
BBa_K749007
1
BBa_K749007
formate dehydrogenase 4 β-subunit from Methylobacterium extorquens
2012-09-25T11:00:00Z
2015-05-08T01:13:12Z
Methylobacterium extorquens
formate dehydrogenase 4 β-subunit from Methylobacterium extorquens
false
false
_1000_
0
8628
9
Not in stock
false
None
false
Takuya Akutsu
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K749015_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggatcgttcgcaaggcctacccaagcgttcttccgccgcgggcggctggggcgcgctgaagagctgcggcaagttcctgttggggagccgggcgccgatctccggcgcacgcgccctgctcagcgccaaccagcccgacggcttcgattgccccggctgcgcctggggcgacccggcccacggctcctcgttcgagttctgcgagaacggcgtgaaggcggtctcctgggaggccaccgacaagcgcgcgacgccgcgtttctttgccaagcatcccgtctcggagctgcgcggctggaccgattacgcgctggagagcgagggccgcctcacgcatccgatgcgctacgacgccgagaccgacacctaccgcgccgtggaatgggacgaggcgttcgccgagatcggcgcgaccctgcgcagcctcgaccatcccgatcgcgtcgagttctacacctccggccgcgcctcgaacgaggcggcctacctctaccagctcttcgcccgggcctacggcaccaacaacttccccgattgctcgaacatgtgccacgaggcgagcggcatcgccctcgtccaggccatcggcatcggcaagggcacggtgctgctcgaagatttcgagaaggcggacgcgatcttcgtcgtcggccagaaccccggcaccaaccatccgcgcatgctgggcgacctgcgtcgggccgccgagcgcggcgcgcgggtcgtggtgctcaaccccgtgcgcgagcgcgggctggagcgtttcgccgatccgcagaacagcgtagagatgctgcgcggggcgagccgcccgatcgccagccattacttccagccgaagcccggcggcgacatggccgcctttcgcggcatcgccaaggtcgtcttcgcccgcgacgcggcggcgatcgaggcgggcaagccctcgctcctcgaccacgccttcatcgccgcgcataccagcgccttcgccgattaccgcgcggcggtcgagacgacggcgtgggacgcgatcctcgaccagtccggcctgacccgcgaggagatcgagaccgcggccgacgtctatctcggcgccgacaaggtgatcgcgacctgggcgatgggcgtgacccagcaccgccactcggtggcgacgatccgcgagatcgccaacctgctgttcctgcgcggccatatcggccggccgggcgcaggcctgtgcccggtgcgcggccattccaacgtgcagggcgaccgcacggtcggcatcaacgagaagccgccgctcgcactcctcgaagcgctcgaccgcgagttcggcctcaacataccgcgcaagcacggccacaacgtgctcggcgccatcggagcgatgctcgacggctcggcgaaggccttcatcgggctcggcggcaatttcgtgcgcgcgacgccggacacgcggctggtggagaaggcgctcgccggctgcgaactcaccgtccacatcgcgacgaagctcaaccactcgcacctcgtgccgggccgggtctcctacctgctgccctgcctcgggcgcaccgaaatcgaccgcaacagccgggccaaggtccagatcgtgaccgtcgaggattcgatgagcatggtccacggctcgggcggcatcaacaagccggcctcgccgcatctgcgctcggagatcggtatcatcgccggcatggccgcggcgacggtgggttcggagcggatcgactgggccgcgctggccgacgattacgacctcatccgtgaccgcatcgagcggacgattccggggttttccggtttcaacacccgggtgcgccggccccgcggcttcatgctgcgcaatctggccgccgagcgggtgttcgagaccgcgaccggacgggcgggcttctccagcgggccgcttccggtggcgaccgagcaccagcgcgcgagccttcggggcgataccttcgtgctgcagaccttccgcagccacgaccagtacaacaccacgatctacggcctcgatgaccgctaccgcggcgtctacggcgagcgccgcgtcgtgttcgccaacccggacgacctcgccgagctgaaggcgcgggcgggcgagcgggtcgatctcgtctgcgtccatgccgaggacggtgtcgagcgggtggcggaggatttccgcctcgtgcccttcgacatgccgcgcggcgcgctcgccggctattatccggaactcaacgtgctggtgccgctctcggccttcggcgaattctcggacacgccgacctcgaagtcggtgctggtccaggtgcgggcccgtgccgccaatgatctgggcaaggccgcgtgatactagaggtgagcgaggaaaaaggccgcgtgagcgagcccgccgccgacgccgagacctttctcgccgcgaccgacacgatcgcggccctgcgtcccgaactcagccttctggaggccggcatcctcgccggcctgcatctcgggctggcggccgacagccggagcttcgcccgcatcttcggcgtcgagcacgccctggtgctgcgggcggtggaaagcctgaccgccgagaccctcatcaccgtgaccgcccgtgacggccgcactcagcggacccgctacgaggcgagcccggcgggcagcgcggtgctgacggcgctcgccgcctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K749007_sequence
1
gtgagcgaggaaaaaggccgcgtgagcgagcccgccgccgacgccgagacctttctcgccgcgaccgacacgatcgcggccctgcgtcccgaactcagccttctggaggccggcatcctcgccggcctgcatctcgggctggcggccgacagccggagcttcgcccgcatcttcggcgtcgagcacgccctggtgctgcgggcggtggaaagcctgaccgccgagaccctcatcaccgtgaccgcccgtgacggccgcactcagcggacccgctacgaggcgagcccggcgggcagcgcggtgctgacggcgctcgccgcctga
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K749006_sequence
1
atggatcgttcgcaaggcctacccaagcgttcttccgccgcgggcggctggggcgcgctgaagagctgcggcaagttcctgttggggagccgggcgccgatctccggcgcacgcgccctgctcagcgccaaccagcccgacggcttcgattgccccggctgcgcctggggcgacccggcccacggctcctcgttcgagttctgcgagaacggcgtgaaggcggtctcctgggaggccaccgacaagcgcgcgacgccgcgtttctttgccaagcatcccgtctcggagctgcgcggctggaccgattacgcgctggagagcgagggccgcctcacgcatccgatgcgctacgacgccgagaccgacacctaccgcgccgtggaatgggacgaggcgttcgccgagatcggcgcgaccctgcgcagcctcgaccatcccgatcgcgtcgagttctacacctccggccgcgcctcgaacgaggcggcctacctctaccagctcttcgcccgggcctacggcaccaacaacttccccgattgctcgaacatgtgccacgaggcgagcggcatcgccctcgtccaggccatcggcatcggcaagggcacggtgctgctcgaagatttcgagaaggcggacgcgatcttcgtcgtcggccagaaccccggcaccaaccatccgcgcatgctgggcgacctgcgtcgggccgccgagcgcggcgcgcgggtcgtggtgctcaaccccgtgcgcgagcgcgggctggagcgtttcgccgatccgcagaacagcgtagagatgctgcgcggggcgagccgcccgatcgccagccattacttccagccgaagcccggcggcgacatggccgcctttcgcggcatcgccaaggtcgtcttcgcccgcgacgcggcggcgatcgaggcgggcaagccctcgctcctcgaccacgccttcatcgccgcgcataccagcgccttcgccgattaccgcgcggcggtcgagacgacggcgtgggacgcgatcctcgaccagtccggcctgacccgcgaggagatcgagaccgcggccgacgtctatctcggcgccgacaaggtgatcgcgacctgggcgatgggcgtgacccagcaccgccactcggtggcgacgatccgcgagatcgccaacctgctgttcctgcgcggccatatcggccggccgggcgcaggcctgtgcccggtgcgcggccattccaacgtgcagggcgaccgcacggtcggcatcaacgagaagccgccgctcgcactcctcgaagcgctcgaccgcgagttcggcctcaacataccgcgcaagcacggccacaacgtgctcggcgccatcggagcgatgctcgacggctcggcgaaggccttcatcgggctcggcggcaatttcgtgcgcgcgacgccggacacgcggctggtggagaaggcgctcgccggctgcgaactcaccgtccacatcgcgacgaagctcaaccactcgcacctcgtgccgggccgggtctcctacctgctgccctgcctcgggcgcaccgaaatcgaccgcaacagccgggccaaggtccagatcgtgaccgtcgaggattcgatgagcatggtccacggctcgggcggcatcaacaagccggcctcgccgcatctgcgctcggagatcggtatcatcgccggcatggccgcggcgacggtgggttcggagcggatcgactgggccgcgctggccgacgattacgacctcatccgtgaccgcatcgagcggacgattccggggttttccggtttcaacacccgggtgcgccggccccgcggcttcatgctgcgcaatctggccgccgagcgggtgttcgagaccgcgaccggacgggcgggcttctccagcgggccgcttccggtggcgaccgagcaccagcgcgcgagccttcggggcgataccttcgtgctgcagaccttccgcagccacgaccagtacaacaccacgatctacggcctcgatgaccgctaccgcggcgtctacggcgagcgccgcgtcgtgttcgccaacccggacgacctcgccgagctgaaggcgcgggcgggcgagcgggtcgatctcgtctgcgtccatgccgaggacggtgtcgagcgggtggcggaggatttccgcctcgtgcccttcgacatgccgcgcggcgcgctcgccggctattatccggaactcaacgtgctggtgccgctctcggccttcggcgaattctcggacacgccgacctcgaagtcggtgctggtccaggtgcgggcccgtgccgccaatgatctgggcaaggccgcgtga
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z