BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K749025 1 BBa_K749025 formaldehyde visualize and dehydrogenate device 2012-09-25T11:00:00Z 2015-05-08T01:13:12Z Pseudomonas putida Escherichia coli Formaldehyde monitoring and dehydrogenate device. The upstream is sensor for formaldehyde and the downstream consecutively produces PFDH, formaldehyde dehydrogenase from Pseudomonas putida. BBa_K749000, by itself, enables to observe the degree of formaldehyde decomposition with the naked eye. false false _1000_ 0 8628 9 It's complicated true none false Takuya Akutsu component2199771 1 BBa_K749021 component2199783 1 BBa_K749024 annotation2199783 1 BBa_K749024 range2199783 1 1233 2630 annotation2199771 1 BBa_K749021 range2199771 1 1 1224 BBa_K749005 1 BBa_K749005 PFDH, glutathion-independent formaldehyde dehydrogenase from Pseudomonas putida 2012-09-25T11:00:00Z 2015-05-08T01:13:12Z Pseudomonas putida PFDH, glutathion-independent formaldehyde dehydrogenase from Pseudomonas putida false false _1000_ 0 8628 9 Not in stock false None false Takuya Akutsu BBa_K749024 1 BBa_K749024 consecutively produces PFDH, formaldehyde dehydrogenase from Pseudomonas putida 2012-09-25T11:00:00Z 2015-05-08T01:13:12Z Pseudomonas putida consecutively produces PFDH, formaldehyde dehydrogenase from Pseudomonas putida false false _1000_ 0 8628 9 Not in stock false none false Takuya Akutsu component2199752 1 BBa_K749005 component2199749 1 BBa_J23100 component2199759 1 BBa_B0015 component2199751 1 BBa_B0034 annotation2199749 1 BBa_J23100 range2199749 1 1 35 annotation2199751 1 BBa_B0034 range2199751 1 44 55 annotation2199759 1 BBa_B0015 range2199759 1 1270 1398 annotation2199752 1 BBa_K749005 range2199752 1 62 1261 BBa_K749008 1 BBa_K749008 frmR repressive promotor 2012-09-25T11:00:00Z 2015-05-08T01:13:12Z Escherichia coli frmR repressive promotor false false _1000_ 0 8628 9 Not in stock false None false Takuya Akutsu BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_K749021 1 BBa_K749021 formaldehyde sensor 2012-09-25T11:00:00Z 2015-05-08T01:13:12Z Escherichia coli formaldehyde sensor false false _1000_ 0 8628 9 It's complicated true none false Takuya Akutsu component2199527 1 BBa_K749004 component2199526 1 BBa_K749008 component2199535 1 BBa_I13401 annotation2199535 1 BBa_I13401 range2199535 1 368 1224 annotation2199526 1 BBa_K749008 range2199526 1 1 79 annotation2199527 1 BBa_K749004 range2199527 1 86 361 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_I13401 1 BBa_I13401 GFP reporter for RHS of library test constructs 2005-04-05T11:00:00Z 2015-08-31T04:07:33Z Released HQ 2013 This part will be suffixed to a promoter.RBS library. The purpose of the experiment is to make a first pass at a library-based construction step to identify efficiency issues and also to examine maxPOPS/RIPS level. false true _6_ 0 2 6 In stock false This part was built by Jen as an intermediate to their nuts and bolts parts. true jasonk component1476713 1 BBa_E0040 component1476728 1 BBa_B0012 component1476718 1 BBa_B0010 annotation1476728 1 BBa_B0012 range1476728 1 817 857 annotation1476718 1 BBa_B0010 range1476718 1 729 808 annotation1476713 1 BBa_E0040 range1476713 1 1 720 BBa_K749004 1 BBa_K749004 frmR, formaldehyde regulator protain 2012-09-25T11:00:00Z 2015-05-08T01:13:12Z frmR [Escherichia coli] formaldehyde regulator protain false false _1000_ 0 8628 9 Not in stock false None false Takuya Akutsu BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K749025_sequence 1 ttatatactataggggggtatgcattgacatatagaatacccccctatagtatattgcatgcagatgatgaggtgcgaatactagatgcccagtactccggaagagaagaaaaaggtccttactcgagttcgtcgtattcgggggcagattgatgctctggaacggtcgctggagggtgatgccgaatgccgtgccatactccaacagatcgctgccgttcggggcgcggctaatgggctgatggcagaagtgcttgaaagccatatccgggaaacgtttgaccgaaatgactgctacagccgcgaagtcagccaatccgttgacgacactattgaactggttcgtgcctatcttaaatagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgtctggcaatcgtggagtggtatatctcggcgccggcaaggtcgaagtgcaacacatcgactacccgaaaatgcaggacccgcgcggcaagaaaatcgaacacggcgtcatcctgaaggtggtctccaccaacatctgcggttctgaccagcacatggtccgtggccgcaccactgcccaggtcggcctggtcctgggccacgaaatcaccggtgaaatcgtcgagaagggccgcgacgtcgagcgcatgcagatcggcgacctggtatcggtaccgttcaacgtcgcctgcggccgctgccgttcctgcaaagagatgcatactggtgtctgcctcaccgtcaacccggcccgcgccggcggtgcctacggctacgtcgacatgggcgactggaccggtggccaggccgaatacgtgctggtgccatacgccgacttcaacctgctgaagctgcctgagcgcgacaaggccatggaaaagatccgtgacctgacctgcctgtccgacatcctgcccaccggctaccacggtgccgtaacggcaggcgtaggcccaggcagcactgtctacgttgccggtgccggcccggtcggcctggccgccgctgcctcggcgcgcctgctgggcgccgcctgcgtaatcgtcggtgacctcaacccggcccgcctggcccacgccaagtcgcaaggcttcgaagtggtcgacctgtccaaggacaccccgctgcacgagcagatcgtcgacatcctcggcgagccggaagtggactgcgctgtcgacgccgtaggcttcgaggcccgtggccacggccatgaaggtgccaagcacgaggccccggccaccgtgcttaactcgctgatgcaagtgacccgcgtggccggcaacatcggtatcccgggcctgtacgtaaccgaagacccaggcgcggtggatgctgcagccaagatcggcgcgctgagcatccgctttggcctgggctgggccaagtcgcacagcttccacaccggccagaccccgaccatgaaatacaaccgccagctgatgcaggcgatcatgtgggatcgcatcaacattgctgaagtggtgggtgtgcaggtgatcaacctggatcaggcgccggaagggtatggcgagtttgatgcgggtgtgcctaagaagtttgtgattgacccgcacaagatgtggggtgcggcgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K749024_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgtctggcaatcgtggagtggtatatctcggcgccggcaaggtcgaagtgcaacacatcgactacccgaaaatgcaggacccgcgcggcaagaaaatcgaacacggcgtcatcctgaaggtggtctccaccaacatctgcggttctgaccagcacatggtccgtggccgcaccactgcccaggtcggcctggtcctgggccacgaaatcaccggtgaaatcgtcgagaagggccgcgacgtcgagcgcatgcagatcggcgacctggtatcggtaccgttcaacgtcgcctgcggccgctgccgttcctgcaaagagatgcatactggtgtctgcctcaccgtcaacccggcccgcgccggcggtgcctacggctacgtcgacatgggcgactggaccggtggccaggccgaatacgtgctggtgccatacgccgacttcaacctgctgaagctgcctgagcgcgacaaggccatggaaaagatccgtgacctgacctgcctgtccgacatcctgcccaccggctaccacggtgccgtaacggcaggcgtaggcccaggcagcactgtctacgttgccggtgccggcccggtcggcctggccgccgctgcctcggcgcgcctgctgggcgccgcctgcgtaatcgtcggtgacctcaacccggcccgcctggcccacgccaagtcgcaaggcttcgaagtggtcgacctgtccaaggacaccccgctgcacgagcagatcgtcgacatcctcggcgagccggaagtggactgcgctgtcgacgccgtaggcttcgaggcccgtggccacggccatgaaggtgccaagcacgaggccccggccaccgtgcttaactcgctgatgcaagtgacccgcgtggccggcaacatcggtatcccgggcctgtacgtaaccgaagacccaggcgcggtggatgctgcagccaagatcggcgcgctgagcatccgctttggcctgggctgggccaagtcgcacagcttccacaccggccagaccccgaccatgaaatacaaccgccagctgatgcaggcgatcatgtgggatcgcatcaacattgctgaagtggtgggtgtgcaggtgatcaacctggatcaggcgccggaagggtatggcgagtttgatgcgggtgtgcctaagaagtttgtgattgacccgcacaagatgtggggtgcggcgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K749008_sequence 1 ttatatactataggggggtatgcattgacatatagaatacccccctatagtatattgcatgcagatgatgaggtgcgaa BBa_I13401_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K749004_sequence 1 atgcccagtactccggaagagaagaaaaaggtccttactcgagttcgtcgtattcgggggcagattgatgctctggaacggtcgctggagggtgatgccgaatgccgtgccatactccaacagatcgctgccgttcggggcgcggctaatgggctgatggcagaagtgcttgaaagccatatccgggaaacgtttgaccgaaatgactgctacagccgcgaagtcagccaatccgttgacgacactattgaactggttcgtgcctatcttaaatag BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K749005_sequence 1 atgtctggcaatcgtggagtggtatatctcggcgccggcaaggtcgaagtgcaacacatcgactacccgaaaatgcaggacccgcgcggcaagaaaatcgaacacggcgtcatcctgaaggtggtctccaccaacatctgcggttctgaccagcacatggtccgtggccgcaccactgcccaggtcggcctggtcctgggccacgaaatcaccggtgaaatcgtcgagaagggccgcgacgtcgagcgcatgcagatcggcgacctggtatcggtaccgttcaacgtcgcctgcggccgctgccgttcctgcaaagagatgcatactggtgtctgcctcaccgtcaacccggcccgcgccggcggtgcctacggctacgtcgacatgggcgactggaccggtggccaggccgaatacgtgctggtgccatacgccgacttcaacctgctgaagctgcctgagcgcgacaaggccatggaaaagatccgtgacctgacctgcctgtccgacatcctgcccaccggctaccacggtgccgtaacggcaggcgtaggcccaggcagcactgtctacgttgccggtgccggcccggtcggcctggccgccgctgcctcggcgcgcctgctgggcgccgcctgcgtaatcgtcggtgacctcaacccggcccgcctggcccacgccaagtcgcaaggcttcgaagtggtcgacctgtccaaggacaccccgctgcacgagcagatcgtcgacatcctcggcgagccggaagtggactgcgctgtcgacgccgtaggcttcgaggcccgtggccacggccatgaaggtgccaagcacgaggccccggccaccgtgcttaactcgctgatgcaagtgacccgcgtggccggcaacatcggtatcccgggcctgtacgtaaccgaagacccaggcgcggtggatgctgcagccaagatcggcgcgctgagcatccgctttggcctgggctgggccaagtcgcacagcttccacaccggccagaccccgaccatgaaatacaaccgccagctgatgcaggcgatcatgtgggatcgcatcaacattgctgaagtggtgggtgtgcaggtgatcaacctggatcaggcgccggaagggtatggcgagtttgatgcgggtgtgcctaagaagtttgtgattgacccgcacaagatgtggggtgcggcgtaa BBa_K749021_sequence 1 ttatatactataggggggtatgcattgacatatagaatacccccctatagtatattgcatgcagatgatgaggtgcgaatactagatgcccagtactccggaagagaagaaaaaggtccttactcgagttcgtcgtattcgggggcagattgatgctctggaacggtcgctggagggtgatgccgaatgccgtgccatactccaacagatcgctgccgttcggggcgcggctaatgggctgatggcagaagtgcttgaaagccatatccgggaaacgtttgaccgaaatgactgctacagccgcgaagtcagccaatccgttgacgacactattgaactggttcgtgcctatcttaaatagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z