BBa_K749025
type: ComponentDefinition
persistent identity: BBa K749025
display id: BBa_K749025
version: 1
was generated by: iGEM to SBOL conversion
description: formaldehyde visualize and dehydrogenate device
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:12Z
mutable provenance: Pseudomonas putida Escherichia coli
owned by: james
owned by: myers
mutable description: Formaldehyde monitoring and dehydrogenate device. The upstream is sensor for formaldehyde and the downstream consecutively produces PFDH, formaldehyde dehydrogenase from Pseudomonas putida. BBa_K749000, by itself, enables to observe the degree of formaldehyde decomposition with the naked eye.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1000_
m user id: 0
owner id: 8628
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: false
creator: Takuya Akutsu
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_K749024
component: BBa_K749021
sequence annotation: BBa_K749021
sequence annotation: BBa_K749024
sequence: https://synbiohub.org/public/igem/BBa_K749025_sequence/1

BBa_K749024
type: Component
persistent identity: component2199783
display id: component2199783
version: 1
top level: BBa_K749025
definition: BBa_K749024
access: public

BBa_K749021
type: Component
persistent identity: component2199771
display id: component2199771
version: 1
top level: BBa_K749025
definition: BBa_K749021
access: public

BBa_K749021
type: SequenceAnnotation
persistent identity: annotation2199771
display id: annotation2199771
version: 1
top level: BBa_K749025
location: https://synbiohub.org/public/igem/BBa_K749025/annotation2199771/range2199771/1
component: BBa_K749021

https://synbiohub.org/public/igem/BBa_K749025/annotation2199771/range2199771/1
type: Range
persistent identity: range2199771
display id: range2199771
version: 1
top level: BBa_K749025
direction: direction/reverse
start: 1
end: 1224
orientation: inline

BBa_K749024
type: SequenceAnnotation
persistent identity: annotation2199783
display id: annotation2199783
version: 1
top level: BBa_K749025
location: https://synbiohub.org/public/igem/BBa_K749025/annotation2199783/range2199783/1
component: BBa_K749024

https://synbiohub.org/public/igem/BBa_K749025/annotation2199783/range2199783/1
type: Range
persistent identity: range2199783
display id: range2199783
version: 1
top level: BBa_K749025
direction: direction/reverse
start: 1233
end: 2630
orientation: inline

BBa_K749021
type: ComponentDefinition
persistent identity: BBa K749021
display id: BBa_K749021
version: 1
was generated by: iGEM to SBOL conversion
description: formaldehyde sensor
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:12Z
mutable provenance: Escherichia coli
owned by: myers
owned by: james
mutable description: formaldehyde sensor
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1000_
m user id: 0
owner id: 8628
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: false
creator: Takuya Akutsu
type: DnaRegion
role: partType/Reporter
role: SO:0000804
component: BBa_I13401
component: BBa_K749004
component: BBa_K749008
sequence annotation: BBa_K749008
sequence annotation: BBa_K749004
sequence annotation: BBa_I13401
sequence: https://synbiohub.org/public/igem/BBa_K749021_sequence/1

BBa_I13401
type: Component
persistent identity: component2199535
display id: component2199535
version: 1
top level: BBa_K749021
definition: BBa_I13401
access: public

BBa_K749004
type: Component
persistent identity: component2199527
display id: component2199527
version: 1
top level: BBa_K749021
definition: BBa_K749004
access: public

BBa_K749008
type: Component
persistent identity: component2199526
display id: component2199526
version: 1
top level: BBa_K749021
definition: BBa_K749008
access: public

BBa_K749008
type: SequenceAnnotation
persistent identity: annotation2199526
display id: annotation2199526
version: 1
top level: BBa_K749021
location: https://synbiohub.org/public/igem/BBa_K749021/annotation2199526/range2199526/1
component: BBa_K749008

https://synbiohub.org/public/igem/BBa_K749021/annotation2199526/range2199526/1
type: Range
persistent identity: range2199526
display id: range2199526
version: 1
top level: BBa_K749021
direction: direction/reverse
start: 1
end: 79
orientation: inline

BBa_K749004
type: SequenceAnnotation
persistent identity: annotation2199527
display id: annotation2199527
version: 1
top level: BBa_K749021
location: https://synbiohub.org/public/igem/BBa_K749021/annotation2199527/range2199527/1
component: BBa_K749004

https://synbiohub.org/public/igem/BBa_K749021/annotation2199527/range2199527/1
type: Range
persistent identity: range2199527
display id: range2199527
version: 1
top level: BBa_K749021
direction: direction/reverse
start: 86
end: 361
orientation: inline

BBa_I13401
type: SequenceAnnotation
persistent identity: annotation2199535
display id: annotation2199535
version: 1
top level: BBa_K749021
location: https://synbiohub.org/public/igem/BBa_K749021/annotation2199535/range2199535/1
component: BBa_I13401

https://synbiohub.org/public/igem/BBa_K749021/annotation2199535/range2199535/1
type: Range
persistent identity: range2199535
display id: range2199535
version: 1
top level: BBa_K749021
direction: direction/reverse
start: 368
end: 1224
orientation: inline

BBa_K749024
type: ComponentDefinition
persistent identity: BBa K749024
display id: BBa_K749024
version: 1
was generated by: iGEM to SBOL conversion
description: consecutively produces PFDH, formaldehyde dehydrogenase from Pseudomonas putida
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:12Z
mutable provenance: Pseudomonas putida
owned by: james
owned by: myers
mutable description: consecutively produces PFDH, formaldehyde dehydrogenase from Pseudomonas putida
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1000_
m user id: 0
owner id: 8628
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: Takuya Akutsu
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K749005
component: BBa_B0034
component: BBa_J23100
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: BBa_K749005
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K749024_sequence/1

BBa_B0015
type: Component
persistent identity: component2199759
display id: component2199759
version: 1
top level: BBa_K749024
definition: BBa_B0015
access: public

BBa_K749005
type: Component
persistent identity: component2199752
display id: component2199752
version: 1
top level: BBa_K749024
definition: BBa_K749005
access: public

BBa_B0034
type: Component
persistent identity: component2199751
display id: component2199751
version: 1
top level: BBa_K749024
definition: BBa_B0034
access: public

BBa_J23100
type: Component
persistent identity: component2199749
display id: component2199749
version: 1
top level: BBa_K749024
definition: BBa_J23100
access: public

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2199749
display id: annotation2199749
version: 1
top level: BBa_K749024
location: https://synbiohub.org/public/igem/BBa_K749024/annotation2199749/range2199749/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K749024/annotation2199749/range2199749/1
type: Range
persistent identity: range2199749
display id: range2199749
version: 1
top level: BBa_K749024
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2199751
display id: annotation2199751
version: 1
top level: BBa_K749024
location: https://synbiohub.org/public/igem/BBa_K749024/annotation2199751/range2199751/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K749024/annotation2199751/range2199751/1
type: Range
persistent identity: range2199751
display id: range2199751
version: 1
top level: BBa_K749024
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K749005
type: SequenceAnnotation
persistent identity: annotation2199752
display id: annotation2199752
version: 1
top level: BBa_K749024
location: https://synbiohub.org/public/igem/BBa_K749024/annotation2199752/range2199752/1
component: BBa_K749005

https://synbiohub.org/public/igem/BBa_K749024/annotation2199752/range2199752/1
type: Range
persistent identity: range2199752
display id: range2199752
version: 1
top level: BBa_K749024
direction: direction/reverse
start: 62
end: 1261
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2199759
display id: annotation2199759
version: 1
top level: BBa_K749024
location: https://synbiohub.org/public/igem/BBa_K749024/annotation2199759/range2199759/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K749024/annotation2199759/range2199759/1
type: Range
persistent identity: range2199759
display id: range2199759
version: 1
top level: BBa_K749024
direction: direction/reverse
start: 1270
end: 1398
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

BBa_I13401
type: ComponentDefinition
persistent identity: BBa I13401
display id: BBa_I13401
version: 1
was generated by: iGEM to SBOL conversion
description: GFP reporter for RHS of library test constructs
created: 2005-04-05T11:00:00Z
modified: 2015-08-31T04:07:33Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part will be suffixed to a promoter.RBS library. The purpose of the experiment is to make a first pass at a library-based construction step to identify efficiency issues and also to examine maxPOPS/RIPS level.
discontinued: false
dominant: true
experience: experience/None
group u list: _6_
m user id: 0
owner id: 2
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part was built by Jen as an intermediate to their nuts and bolts parts.
star: true
creator: jasonk
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_E0040
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13401_sequence/1

BBa_E0040
type: Component
persistent identity: component1476713
display id: component1476713
version: 1
top level: BBa_I13401
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1476728
display id: component1476728
version: 1
top level: BBa_I13401
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1476718
display id: component1476718
version: 1
top level: BBa_I13401
definition: BBa_B0010
access: public

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1476713
display id: annotation1476713
version: 1
top level: BBa_I13401
location: https://synbiohub.org/public/igem/BBa_I13401/annotation1476713/range1476713/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I13401/annotation1476713/range1476713/1
type: Range
persistent identity: range1476713
display id: range1476713
version: 1
top level: BBa_I13401
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1476718
display id: annotation1476718
version: 1
top level: BBa_I13401
location: https://synbiohub.org/public/igem/BBa_I13401/annotation1476718/range1476718/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13401/annotation1476718/range1476718/1
type: Range
persistent identity: range1476718
display id: range1476718
version: 1
top level: BBa_I13401
direction: direction/forward
start: 729
end: 808
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1476728
display id: annotation1476728
version: 1
top level: BBa_I13401
location: https://synbiohub.org/public/igem/BBa_I13401/annotation1476728/range1476728/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13401/annotation1476728/range1476728/1
type: Range
persistent identity: range1476728
display id: range1476728
version: 1
top level: BBa_I13401
direction: direction/forward
start: 817
end: 857
orientation: inline

BBa_K749004
type: ComponentDefinition
persistent identity: BBa K749004
display id: BBa_K749004
version: 1
was generated by: iGEM to SBOL conversion
description: frmR, formaldehyde regulator protain
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:12Z
mutable provenance: frmR [Escherichia coli]
owned by: james
owned by: myers
mutable description: formaldehyde regulator protain
discontinued: false
dominant: false
experience: experience/None
group u list: _1000_
m user id: 0
owner id: 8628
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Takuya Akutsu
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K749004_sequence/1

BBa_K749005
type: ComponentDefinition
persistent identity: BBa K749005
display id: BBa_K749005
version: 1
was generated by: iGEM to SBOL conversion
description: PFDH, glutathion-independent formaldehyde dehydrogenase from Pseudomonas putida
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:12Z
mutable provenance: Pseudomonas putida
owned by: james
owned by: myers
mutable description: PFDH, glutathion-independent formaldehyde dehydrogenase from Pseudomonas putida
discontinued: false
dominant: false
group u list: _1000_
m user id: 0
owner id: 8628
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Takuya Akutsu
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K749005_sequence/1

BBa_K749008
type: ComponentDefinition
persistent identity: BBa K749008
display id: BBa_K749008
version: 1
was generated by: iGEM to SBOL conversion
description: frmR repressive promotor
created: 2012-09-25T11:00:00Z
modified: 2015-05-08T01:13:12Z
mutable provenance: Escherichia coli
owned by: james
owned by: myers
mutable description: frmR repressive promotor
discontinued: false
dominant: false
group u list: _1000_
m user id: 0
owner id: 8628
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Takuya Akutsu
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K749008_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

https://synbiohub.org/public/igem/BBa_K749025_sequence/1
type: Sequence
persistent identity: BBa K749025 sequence
display id: BBa_K749025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatatactataggggggtatgcattgacatatagaatacccccctatagtatattgcatgcagatgatgaggtgcgaatactagatgcccagtactccggaagagaagaaaaaggtccttactcgagttcgtcgtattcgggggcagattgatgctctggaacggtcgctggagggtgatgccgaatgccgtgccatactccaacagatcgctgccgttcggggcgcggctaatgggctgatggcagaagtgcttgaaagccatatccgggaaacgtttgaccgaaatgactgctacagccgcgaagtcagccaatccgttgacgacactattgaactggttcgtgcctatcttaaatagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgtctggcaatcgtggagtggtatatctcggcgccggcaaggtcgaagtgcaacacatcgactacccgaaaatgcaggacccgcgcggcaagaaaatcgaacacggcgtcatcctgaaggtggtctccaccaacatctgcggttctgaccagcacatggtccgtggccgcaccactgcccaggtcggcctggtcctgggccacgaaatcaccggtgaaatcgtcgagaagggccgcgacgtcgagcgcatgcagatcggcgacctggtatcggtaccgttcaacgtcgcctgcggccgctgccgttcctgcaaagagatgcatactggtgtctgcctcaccgtcaacccggcccgcgccggcggtgcctacggctacgtcgacatgggcgactggaccggtggccaggccgaatacgtgctggtgccatacgccgacttcaacctgctgaagctgcctgagcgcgacaaggccatggaaaagatccgtgacctgacctgcctgtccgacatcctgcccaccggctaccacggtgccgtaacggcaggcgtaggcccaggcagcactgtctacgttgccggtgccggcccggtcggcctggccgccgctgcctcggcgcgcctgctgggcgccgcctgcgtaatcgtcggtgacctcaacccggcccgcctggcccacgccaagtcgcaaggcttcgaagtggtcga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K749021_sequence/1
type: Sequence
persistent identity: BBa K749021 sequence
display id: BBa_K749021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatatactataggggggtatgcattgacatatagaatacccccctatagtatattgcatgcagatgatgaggtgcgaatactagatgcccagtactccggaagagaagaaaaaggtccttactcgagttcgtcgtattcgggggcagattgatgctctggaacggtcgctggagggtgatgccgaatgccgtgccatactccaacagatcgctgccgttcggggcgcggctaatgggctgatggcagaagtgcttgaaagccatatccgggaaacgtttgaccgaaatgactgctacagccgcgaagtcagccaatccgttgacgacactattgaactggttcgtgcctatcttaaatagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K749024_sequence/1
type: Sequence
persistent identity: BBa K749024 sequence
display id: BBa_K749024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgtctggcaatcgtggagtggtatatctcggcgccggcaaggtcgaagtgcaacacatcgactacccgaaaatgcaggacccgcgcggcaagaaaatcgaacacggcgtcatcctgaaggtggtctccaccaacatctgcggttctgaccagcacatggtccgtggccgcaccactgcccaggtcggcctggtcctgggccacgaaatcaccggtgaaatcgtcgagaagggccgcgacgtcgagcgcatgcagatcggcgacctggtatcggtaccgttcaacgtcgcctgcggccgctgccgttcctgcaaagagatgcatactggtgtctgcctcaccgtcaacccggcccgcgccggcggtgcctacggctacgtcgacatgggcgactggaccggtggccaggccgaatacgtgctggtgccatacgccgacttcaacctgctgaagctgcctgagcgcgacaaggccatggaaaagatccgtgacctgacctgcctgtccgacatcctgcccaccggctaccacggtgccgtaacggcaggcgtaggcccaggcagcactgtctacgttgccggtgccggcccggtcggcctggccgccgctgcctcggcgcgcctgctgggcgccgcctgcgtaatcgtcggtgacctcaacccggcccgcctggcccacgccaagtcgcaaggcttcgaagtggtcgacctgtccaaggacaccccgctgcacgagcagatcgtcgacatcctcggcgagccggaagtggactgcgctgtcgacgccgtaggcttcgaggcccgtggccacggccatgaaggtgccaagcacgaggccccggccaccgtgcttaactcgctgatgcaagtgacccgcgtggccggcaacatcggtatcccgggcctgtacgtaaccgaagacccaggcgcggtggatgctgcagccaagatcggcgcgctgagcatccgctttggcctgggctgggccaagtcgcacagcttccacaccggccagaccccgaccatgaaatacaaccgccagctgatgcaggcgatcatgtgggatcgcatcaacattgctgaagtggtgggtgtgcaggtgatcaacctggatcaggcgccggaagggtatggcgagtttgatgcgggtgtgcctaagaagtttgtgattgacccgcacaagatgtggggtgcggcgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13401_sequence/1
type: Sequence
persistent identity: BBa I13401 sequence
display id: BBa_I13401_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K749004_sequence/1
type: Sequence
persistent identity: BBa K749004 sequence
display id: BBa_K749004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcccagtactccggaagagaagaaaaaggtccttactcgagttcgtcgtattcgggggcagattgatgctctggaacggtcgctggagggtgatgccgaatgccgtgccatactccaacagatcgctgccgttcggggcgcggctaatgggctgatggcagaagtgcttgaaagccatatccgggaaacgtttgaccgaaatgactgctacagccgcgaagtcagccaatccgttgacgacactattgaactggttcgtgcctatcttaaatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K749005_sequence/1
type: Sequence
persistent identity: BBa K749005 sequence
display id: BBa_K749005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtctggcaatcgtggagtggtatatctcggcgccggcaaggtcgaagtgcaacacatcgactacccgaaaatgcaggacccgcgcggcaagaaaatcgaacacggcgtcatcctgaaggtggtctccaccaacatctgcggttctgaccagcacatggtccgtggccgcaccactgcccaggtcggcctggtcctgggccacgaaatcaccggtgaaatcgtcgagaagggccgcgacgtcgagcgcatgcagatcggcgacctggtatcggtaccgttcaacgtcgcctgcggccgctgccgttcctgcaaagagatgcatactggtgtctgcctcaccgtcaacccggcccgcgccggcggtgcctacggctacgtcgacatgggcgactggaccggtggccaggccgaatacgtgctggtgccatacgccgacttcaacctgctgaagctgcctgagcgcgacaaggccatggaaaagatccgtgacctgacctgcctgtccgacatcctgcccaccggctaccacggtgccgtaacggcaggcgtaggcccaggcagcactgtctacgttgccggtgccggcccggtcggcctggccgccgctgcctcggcgcgcctgctgggcgccgcctgcgtaatcgtcggtgacctcaacccggcccgcctggcccacgccaagtcgcaaggcttcgaagtggtcgacctgtccaaggacaccccgctgcacgagcagatcgtcgacatcctcggcgagccggaagtggactgcgctgtcgacgccgtaggcttcgaggcccgtggccacggccatgaaggtgccaagcacgaggccccggccaccgtgcttaactcgctgatgcaagtgacccgcgtggccggcaacatcggtatcccgggcctgtacgtaaccgaagacccaggcgcggtggatgctgcagccaagatcggcgcgctgagcatccgctttggcctgggctgggccaagtcgcacagcttccacaccggccagaccccgaccatgaaatacaaccgccagctgatgcaggcgatcatgtgggatcgcatcaacattgctgaagtggtgggtgtgcaggtgatcaacctggatcaggcgccggaagggtatggcgagtttgatgcgggtgtgcctaagaagtttgtgattgacccgcacaagatgtggggtgcggcgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K749008_sequence/1
type: Sequence
persistent identity: BBa K749008 sequence
display id: BBa_K749008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatatactataggggggtatgcattgacatatagaatacccccctatagtatattgcatgcagatgatgaggtgcgaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z