LOCUS       BBa_K750007             2020 bp    DNA     linear       25-Nov-2024
DEFINITION  LuxR->LuxPR->GFP
ACCESSION   BBa_K750007
VERSION     BBa_K750007.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    157..158
                     /label=prefix
     misc_feature    330..330
                     /label=T
     misc_feature    648..648
                     /label=A
     CDS             157..906
                     /label=luxR
     misc_feature    157..912
                     /label=BBa_C0062
     misc_feature    913..937
                     /label=Help:Barcodes
     CDS             157..912
                     /label=BBa_C0062
     stem_loop       957..1000
                     /label=stem_loop
     misc_feature    946..1025
                     /label=BBa_B0010
     terminator      946..1025
                     /label=BBa_B0010
     stem_loop       1041..1060
                     /label=T7 TE
     misc_feature    1067..1067
                     /label=stop
     polyA_site      1061..1074
                     /label=polya
     misc_feature    1034..1074
                     /label=BBa_B0012
     terminator      1034..1074
                     /label=BBa_B0012
     terminator      946..1074
                     /label=BBa_B0015
     misc_feature    1..1074
                     /label=BBa_K750006
     misc_feature    1083..1102
                     /label=LuxR/HSL
     promoter        1102..1107
                     /label=-35
     promoter        1124..1129
                     /label=-10
     misc_feature    1135..1135
                     /label=start
     misc_feature    1083..1137
                     /label=BBa_R0062
     promoter        1083..1137
                     /label=BBa_R0062
     misc_feature    1150..1153
                     /label=conserved
     RBS             1146..1157
                     /label=BBa_B0034
     CDS             1164..1883
                     /label=GFP protein
     CDS             1164..1883
                     /label=BBa_E0040
     stem_loop       1903..1946
                     /label=stem_loop
     misc_feature    1892..1971
                     /label=BBa_B0010
     terminator      1892..1971
                     /label=BBa_B0010
     stem_loop       1987..2006
                     /label=T7 TE
     misc_feature    2013..2013
                     /label=stop
     polyA_site      2007..2020
                     /label=polya
     misc_feature    1980..2020
                     /label=BBa_B0012
     terminator      1980..2020
                     /label=BBa_B0012
     terminator      1892..2020
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga aaaacataaa tgccgacgac
      181 acatacagaa taattaataa aattaaagct tgtagaagca ataatgatat taatcaatgc
      241 ttatctgata tgactaaaat ggtacattgt gaatattatt tactcgcgat catttatcct
      301 cattctatgg ttaaatctga tatttcaatc ctagataatt accctaaaaa atggaggcaa
      361 tattatgatg acgctaattt aataaaatat gatcctatag tagattattc taactccaat
      421 cattcaccaa ttaattggaa tatatttgaa aacaatgctg taaataaaaa atctccaaat
      481 gtaattaaag aagcgaaaac atcaggtctt atcactgggt ttagtttccc tattcatacg
      541 gctaacaatg gcttcggaat gcttagtttt gcacattcag aaaaagacaa ctatatagat
      601 agtttatttt tacatgcgtg tatgaacata ccattaattg ttccttctct agttgataat
      661 tatcgaaaaa taaatatagc aaataataaa tcaaacaacg atttaaccaa aagagaaaaa
      721 gaatgtttag cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa aatattaggt
      781 tgcagtgagc gtactgtcac tttccattta accaatgcgc aaatgaaact caatacaaca
      841 aaccgctgcc aaagtatttc taaagcaatt ttaacaggag caattgattg cccatacttt
      901 aaaaattaat aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa
      961 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1021 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
     1081 agacctgtag gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataaatac
     1141 tagagaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc
     1201 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     1261 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     1321 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
     1381 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
     1441 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
     1501 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
     1561 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
     1621 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
     1681 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
     1741 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
     1801 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     1861 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag
     1921 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1981 cacactggct caccttcggg tgggcctttc tgcgtttata
//