LOCUS BBa_K750009 2964 bp DNA linear 30-Apr-2024 DEFINITION TIME DELAY1.0:LuxI(RBS1.0)->LuxR->LuxPR->GFP ACCESSION BBa_K750009 VERSION BBa_K750009.1 FEATURES Location/Qualifiers promoter 1..131 /label=promoter misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 promoter 1..130 /label=BBa_K206000 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 misc_feature 736..767 /label=LVA CDS 157..735 /label=luxI misc_feature 157..774 /label=BBa_C0061 misc_feature 775..799 /label=Help:Barcodes CDS 157..774 /label=BBa_C0061 stem_loop 819..862 /label=stem_loop misc_feature 808..887 /label=BBa_B0010 terminator 808..887 /label=BBa_B0010 stem_loop 903..922 /label=T7 TE misc_feature 929..929 /label=stop polyA_site 923..936 /label=polya misc_feature 896..936 /label=BBa_B0012 terminator 896..936 /label=BBa_B0012 terminator 808..936 /label=BBa_B0015 misc_feature 1..936 /label=BBa_K750001 promoter 945..1075 /label=promoter misc_feature 984..1001 /label=AraI1 misc_feature 1005..1022 /label=AraI2 promoter 945..1074 /label=BBa_K206000 misc_feature 1087..1090 /label=conserved RBS 1083..1094 /label=BBa_B0034 misc_feature 1101..1102 /label=prefix misc_feature 1274..1274 /label=T misc_feature 1592..1592 /label=A CDS 1101..1850 /label=luxR misc_feature 1101..1856 /label=BBa_C0062 misc_feature 1857..1881 /label=Help:Barcodes CDS 1101..1856 /label=BBa_C0062 stem_loop 1901..1944 /label=stem_loop misc_feature 1890..1969 /label=BBa_B0010 terminator 1890..1969 /label=BBa_B0010 stem_loop 1985..2004 /label=T7 TE misc_feature 2011..2011 /label=stop polyA_site 2005..2018 /label=polya misc_feature 1978..2018 /label=BBa_B0012 terminator 1978..2018 /label=BBa_B0012 terminator 1890..2018 /label=BBa_B0015 misc_feature 945..2018 /label=BBa_K750006 misc_feature 2027..2046 /label=LuxR/HSL promoter 2046..2051 /label=-35 promoter 2068..2073 /label=-10 misc_feature 2079..2079 /label=start misc_feature 2027..2081 /label=BBa_R0062 promoter 2027..2081 /label=BBa_R0062 misc_feature 2094..2097 /label=conserved RBS 2090..2101 /label=BBa_B0034 CDS 2108..2827 /label=GFP protein CDS 2108..2827 /label=BBa_E0040 stem_loop 2847..2890 /label=stem_loop misc_feature 2836..2915 /label=BBa_B0010 terminator 2836..2915 /label=BBa_B0010 stem_loop 2931..2950 /label=T7 TE misc_feature 2957..2957 /label=stop polyA_site 2951..2964 /label=polya misc_feature 2924..2964 /label=BBa_B0012 terminator 2924..2964 /label=BBa_B0012 terminator 2836..2964 /label=BBa_B0015 misc_feature 945..2964 /label=BBa_K750007 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatga ctataatgat aaaaaaatcg 181 gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg ttatcaagtg 241 tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc agatgagtat 301 gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt aagtggatgc 361 tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc tgaattgctt 421 ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt tgctgtaggt 481 aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact atttgaagct 541 atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac atcaacagca 601 atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga caaagaaatt 661 catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga acagtttaaa 721 aaagcagtct taaatgctgc aaacgacgaa aactacgctt tagtagctta ataactctga 781 tagtgctagt gtagatctct actagagcca ggcatcaaat aaaacgaaag gctcagtcga 841 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 901 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagacattg attatttgca 961 cggcgtcaca ctttgctatg ccatagcaag atagtccata agattagcgg atcctacctg 1021 acgcttttta tcgcaactct ctactgtttc tccataccgt ttttttgggc tagctactag 1081 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 1141 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 1201 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 1261 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 1321 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 1381 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 1441 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 1501 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 1561 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 1621 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 1681 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 1741 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 1801 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 1861 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 1921 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 1981 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 2041 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa 2101 atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt 2161 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac 2221 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc 2281 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat 2341 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat 2401 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac 2461 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg 2521 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa 2581 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact 2641 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa 2701 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat 2761 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa 2821 ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 2881 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 2941 cgggtgggcc tttctgcgtt tata //