LOCUS BBa_K750010 2967 bp DNA linear 07-May-2024 DEFINITION TIME DELAY 0.6:LuxI(RBS0.6)->LuxR->LuxPR->GFP ACCESSION BBa_K750010 VERSION BBa_K750010.1 FEATURES Location/Qualifiers promoter 1..131 /label=promoter misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 promoter 1..130 /label=BBa_K206000 RBS 139..153 /label=RBS-1\Strong RBS 146..150 /label=RBS misc_feature 139..153 /label=BBa_B0030 RBS 139..153 /label=BBa_B0030 misc_feature 739..770 /label=LVA CDS 160..738 /label=luxI misc_feature 160..777 /label=BBa_C0061 misc_feature 778..802 /label=Help:Barcodes CDS 160..777 /label=BBa_C0061 stem_loop 822..865 /label=stem_loop misc_feature 811..890 /label=BBa_B0010 terminator 811..890 /label=BBa_B0010 stem_loop 906..925 /label=T7 TE misc_feature 932..932 /label=stop polyA_site 926..939 /label=polya misc_feature 899..939 /label=BBa_B0012 terminator 899..939 /label=BBa_B0012 terminator 811..939 /label=BBa_B0015 misc_feature 1..939 /label=BBa_K750002 promoter 948..1078 /label=promoter misc_feature 987..1004 /label=AraI1 misc_feature 1008..1025 /label=AraI2 promoter 948..1077 /label=BBa_K206000 misc_feature 1090..1093 /label=conserved RBS 1086..1097 /label=BBa_B0034 misc_feature 1104..1105 /label=prefix misc_feature 1277..1277 /label=T misc_feature 1595..1595 /label=A CDS 1104..1853 /label=luxR misc_feature 1104..1859 /label=BBa_C0062 misc_feature 1860..1884 /label=Help:Barcodes CDS 1104..1859 /label=BBa_C0062 stem_loop 1904..1947 /label=stem_loop misc_feature 1893..1972 /label=BBa_B0010 terminator 1893..1972 /label=BBa_B0010 stem_loop 1988..2007 /label=T7 TE misc_feature 2014..2014 /label=stop polyA_site 2008..2021 /label=polya misc_feature 1981..2021 /label=BBa_B0012 terminator 1981..2021 /label=BBa_B0012 terminator 1893..2021 /label=BBa_B0015 misc_feature 948..2021 /label=BBa_K750006 misc_feature 2030..2049 /label=LuxR/HSL promoter 2049..2054 /label=-35 promoter 2071..2076 /label=-10 misc_feature 2082..2082 /label=start misc_feature 2030..2084 /label=BBa_R0062 promoter 2030..2084 /label=BBa_R0062 misc_feature 2097..2100 /label=conserved RBS 2093..2104 /label=BBa_B0034 CDS 2111..2830 /label=GFP protein CDS 2111..2830 /label=BBa_E0040 stem_loop 2850..2893 /label=stem_loop misc_feature 2839..2918 /label=BBa_B0010 terminator 2839..2918 /label=BBa_B0010 stem_loop 2934..2953 /label=T7 TE misc_feature 2960..2960 /label=stop polyA_site 2954..2967 /label=polya misc_feature 2927..2967 /label=BBa_B0012 terminator 2927..2967 /label=BBa_B0012 terminator 2839..2967 /label=BBa_B0015 misc_feature 948..2967 /label=BBa_K750007 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagat taaagaggag aaatactaga tgactataat gataaaaaaa 181 tcggattttt tggcaattcc atcggaggag tataaaggta ttctaagtct tcgttatcaa 241 gtgtttaagc aaagacttga gtgggactta gttgtagaaa ataaccttga atcagatgag 301 tatgataact caaatgcaga atatatttat gcttgtgatg atactgaaaa tgtaagtgga 361 tgctggcgtt tattacctac aacaggtgat tatatgctga aaagtgtttt tcctgaattg 421 cttggtcaac agagtgctcc caaagatcct aatatagtcg aattaagtcg ttttgctgta 481 ggtaaaaata gctcaaagat aaataactct gctagtgaaa ttacaatgaa actatttgaa 541 gctatatata aacacgctgt tagtcaaggt attacagaat atgtaacagt aacatcaaca 601 gcaatagagc gatttttaaa gcgtattaaa gttccttgtc atcgtattgg agacaaagaa 661 attcatgtat taggtgatac taaatcggtt gtattgtcta tgcctattaa tgaacagttt 721 aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg ctttagtagc ttaataactc 781 tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt 841 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 901 acactggctc accttcgggt gggcctttct gcgtttatat actagagaca ttgattattt 961 gcacggcgtc acactttgct atgccatagc aagatagtcc ataagattag cggatcctac 1021 ctgacgcttt ttatcgcaac tctctactgt ttctccatac cgtttttttg ggctagctac 1081 tagagaaaga ggagaaatac tagatgaaaa acataaatgc cgacgacaca tacagaataa 1141 ttaataaaat taaagcttgt agaagcaata atgatattaa tcaatgctta tctgatatga 1201 ctaaaatggt acattgtgaa tattatttac tcgcgatcat ttatcctcat tctatggtta 1261 aatctgatat ttcaatccta gataattacc ctaaaaaatg gaggcaatat tatgatgacg 1321 ctaatttaat aaaatatgat cctatagtag attattctaa ctccaatcat tcaccaatta 1381 attggaatat atttgaaaac aatgctgtaa ataaaaaatc tccaaatgta attaaagaag 1441 cgaaaacatc aggtcttatc actgggttta gtttccctat tcatacggct aacaatggct 1501 tcggaatgct tagttttgca cattcagaaa aagacaacta tatagatagt ttatttttac 1561 atgcgtgtat gaacatacca ttaattgttc cttctctagt tgataattat cgaaaaataa 1621 atatagcaaa taataaatca aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt 1681 gggcatgcga aggaaaaagc tcttgggata tttcaaaaat attaggttgc agtgagcgta 1741 ctgtcacttt ccatttaacc aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa 1801 gtatttctaa agcaatttta acaggagcaa ttgattgccc atactttaaa aattaataac 1861 actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca 1921 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 1981 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga cctgtaggat 2041 cgtacaggtt tacgcaagaa aatggtttgt tatagtcgaa taaatactag agaaagagga 2101 gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga 2161 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc 2221 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg 2281 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca 2341 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac 2401 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga 2461 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct 2521 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca 2581 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca 2641 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga 2701 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca 2761 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata 2821 caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 2881 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 2941 cttcgggtgg gcctttctgc gtttata //