##gff-version 3
##sequence-region BBa_K750010 1 2967
BBa_K750010	.	engineered_region	1	939	.	+	0	ID=BBa_K750002;Name=BBa_K750002
BBa_K750010	.	terminator	811	939	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750002
BBa_K750010	.	terminator	899	939	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750010	.	polyA_site	926	939	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750010	.	stem_loop	906	925	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750010	.	engineered_region	899	939	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750010	.	stop_codon	932	932	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750010	.	terminator	811	890	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750010	.	stem_loop	822	865	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750010	.	engineered_region	811	890	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750010	.	promoter	1	130	.	+	0	ID=BBa_K206000;Name=BBa_K206000;Parent=BBa_K750002
BBa_K750010	.	operator	40	57	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K750010	.	promoter	1	131	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K750010	.	operator	61	78	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K750010	.	ribosome_entry_site	139	153	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K750002
BBa_K750010	.	ribosome_entry_site	146	150	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K750010	.	ribosome_entry_site	139	153	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K750010	.	engineered_region	139	153	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K750010	.	CDS	160	777	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K750002
BBa_K750010	.	tag	739	770	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K750010	.	engineered_region	160	777	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K750010	.	engineered_tag	778	802	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K750010	.	CDS	160	738	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K750010	.	engineered_region	948	2967	.	+	0	ID=BBa_K750007;Name=BBa_K750007
BBa_K750010	.	engineered_region	948	2021	.	+	0	ID=BBa_K750006;Name=BBa_K750006;Parent=BBa_K750007
BBa_K750010	.	terminator	1893	2021	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750006
BBa_K750010	.	terminator	1981	2021	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750010	.	polyA_site	2008	2021	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750010	.	stem_loop	1988	2007	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750010	.	engineered_region	1981	2021	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750010	.	stop_codon	2014	2014	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750010	.	terminator	1893	1972	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750010	.	stem_loop	1904	1947	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750010	.	engineered_region	1893	1972	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750010	.	ribosome_entry_site	1086	1097	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K750006
BBa_K750010	.	conserved_region	1090	1093	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750010	.	CDS	1104	1859	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K750006
BBa_K750010	.	engineered_region	1104	1859	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K750010	.	engineered_tag	1860	1884	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K750010	.	engineered_region	1104	1105	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K750010	.	point_mutation	1595	1595	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K750010	.	CDS	1104	1853	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K750010	.	point_mutation	1277	1277	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K750010	.	promoter	948	1077	.	+	0	ID=BBa_K206000;Name=BBa_K206000;Parent=BBa_K750006
BBa_K750010	.	operator	987	1004	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K750010	.	promoter	948	1078	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K750010	.	operator	1008	1025	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K750010	.	ribosome_entry_site	2093	2104	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K750007
BBa_K750010	.	conserved_region	2097	2100	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750010	.	CDS	2111	2830	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K750007
BBa_K750010	.	CDS	2111	2830	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K750010	.	promoter	2030	2084	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K750007
BBa_K750010	.	promoter	2049	2054	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K750010	.	engineered_region	2030	2084	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K750010	.	promoter	2071	2076	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K750010	.	start_codon	2082	2082	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K750010	.	non_covalent_binding_site	2030	2049	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K750010	.	terminator	2839	2967	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750007
BBa_K750010	.	terminator	2927	2967	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750010	.	polyA_site	2954	2967	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750010	.	stem_loop	2934	2953	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750010	.	engineered_region	2927	2967	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750010	.	stop_codon	2960	2960	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750010	.	terminator	2839	2918	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750010	.	stem_loop	2850	2893	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750010	.	engineered_region	2839	2918	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K750010
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagattaaagaggagaaatacta
gatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttat
caagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatg
cagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatat
gctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgtttt
gctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaac
acgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagt
tccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaat
gaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgct
agtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaga
cattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgc
tttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatg
aaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaat
gcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatc
tgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcct
atagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctc
caaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggctt
cggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatacca
ttaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaa
gagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcg
tactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaatt
ttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacg
caagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgcgtaaaggagaagaactttt
cactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaa
ggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttg
tcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgc
catgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaag
tttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacaca
aattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaa
aattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccct
gtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatgg
tccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata