LOCUS BBa_K750011 2965 bp DNA linear 26-Dec-2024 DEFINITION TIME DELAY0.3:LuxI(RBS0.3)->LuxR->LuxPR->GFP ACCESSION BBa_K750011 VERSION BBa_K750011.1 FEATURES Location/Qualifiers promoter 1..131 /label=promoter misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 promoter 1..130 /label=BBa_K206000 RBS 139..151 /label=RBS-3\Weak RBS 145..148 /label=RBS misc_feature 139..151 /label=BBa_B0032 RBS 139..151 /label=BBa_B0032 misc_feature 737..768 /label=LVA CDS 158..736 /label=luxI misc_feature 158..775 /label=BBa_C0061 misc_feature 776..800 /label=Help:Barcodes CDS 158..775 /label=BBa_C0061 stem_loop 820..863 /label=stem_loop misc_feature 809..888 /label=BBa_B0010 terminator 809..888 /label=BBa_B0010 stem_loop 904..923 /label=T7 TE misc_feature 930..930 /label=stop polyA_site 924..937 /label=polya misc_feature 897..937 /label=BBa_B0012 terminator 897..937 /label=BBa_B0012 terminator 809..937 /label=BBa_B0015 misc_feature 1..937 /label=BBa_K750003 promoter 946..1076 /label=promoter misc_feature 985..1002 /label=AraI1 misc_feature 1006..1023 /label=AraI2 promoter 946..1075 /label=BBa_K206000 misc_feature 1088..1091 /label=conserved RBS 1084..1095 /label=BBa_B0034 misc_feature 1102..1103 /label=prefix misc_feature 1275..1275 /label=T misc_feature 1593..1593 /label=A CDS 1102..1851 /label=luxR misc_feature 1102..1857 /label=BBa_C0062 misc_feature 1858..1882 /label=Help:Barcodes CDS 1102..1857 /label=BBa_C0062 stem_loop 1902..1945 /label=stem_loop misc_feature 1891..1970 /label=BBa_B0010 terminator 1891..1970 /label=BBa_B0010 stem_loop 1986..2005 /label=T7 TE misc_feature 2012..2012 /label=stop polyA_site 2006..2019 /label=polya misc_feature 1979..2019 /label=BBa_B0012 terminator 1979..2019 /label=BBa_B0012 terminator 1891..2019 /label=BBa_B0015 misc_feature 946..2019 /label=BBa_K750006 misc_feature 2028..2047 /label=LuxR/HSL promoter 2047..2052 /label=-35 promoter 2069..2074 /label=-10 misc_feature 2080..2080 /label=start misc_feature 2028..2082 /label=BBa_R0062 promoter 2028..2082 /label=BBa_R0062 misc_feature 2095..2098 /label=conserved RBS 2091..2102 /label=BBa_B0034 CDS 2109..2828 /label=GFP protein CDS 2109..2828 /label=BBa_E0040 stem_loop 2848..2891 /label=stem_loop misc_feature 2837..2916 /label=BBa_B0010 terminator 2837..2916 /label=BBa_B0010 stem_loop 2932..2951 /label=T7 TE misc_feature 2958..2958 /label=stop polyA_site 2952..2965 /label=polya misc_feature 2925..2965 /label=BBa_B0012 terminator 2925..2965 /label=BBa_B0012 terminator 2837..2965 /label=BBa_B0015 misc_feature 946..2965 /label=BBa_K750007 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagtc acacaggaaa gtactagatg actataatga taaaaaaatc 181 ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc gttatcaagt 241 gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat cagatgagta 301 tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg taagtggatg 361 ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc ctgaattgct 421 tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt ttgctgtagg 481 taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac tatttgaagc 541 tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa catcaacagc 601 aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag acaaagaaat 661 tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg aacagtttaa 721 aaaagcagtc ttaaatgctg caaacgacga aaactacgct ttagtagctt aataactctg 781 atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 841 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 901 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagacatt gattatttgc 961 acggcgtcac actttgctat gccatagcaa gatagtccat aagattagcg gatcctacct 1021 gacgcttttt atcgcaactc tctactgttt ctccataccg tttttttggg ctagctacta 1081 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 1141 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 1201 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 1261 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 1321 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 1381 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 1441 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 1501 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 1561 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 1621 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 1681 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 1741 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 1801 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 1861 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 1921 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 1981 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 2041 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga 2101 aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat 2161 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa 2221 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc 2281 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata 2341 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta 2401 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata 2461 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg 2521 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa 2581 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac 2641 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca 2701 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca 2761 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca 2821 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 2881 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 2941 tcgggtgggc ctttctgcgt ttata //