##gff-version 3
##sequence-region BBa_K750011 1 2965
BBa_K750011	.	engineered_region	1	937	.	+	0	ID=BBa_K750003;Name=BBa_K750003
BBa_K750011	.	terminator	809	937	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750003
BBa_K750011	.	terminator	897	937	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750011	.	stem_loop	904	923	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750011	.	polyA_site	924	937	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750011	.	stop_codon	930	930	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750011	.	engineered_region	897	937	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750011	.	terminator	809	888	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750011	.	stem_loop	820	863	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750011	.	engineered_region	809	888	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750011	.	ribosome_entry_site	139	151	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K750003
BBa_K750011	.	engineered_region	139	151	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K750011	.	ribosome_entry_site	145	148	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K750011	.	ribosome_entry_site	139	151	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K750011	.	CDS	158	775	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K750003
BBa_K750011	.	engineered_region	158	775	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K750011	.	engineered_tag	776	800	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K750011	.	CDS	158	736	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K750011	.	tag	737	768	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K750011	.	promoter	1	130	.	+	0	ID=BBa_K206000;Name=BBa_K206000;Parent=BBa_K750003
BBa_K750011	.	operator	61	78	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K750011	.	promoter	1	131	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K750011	.	operator	40	57	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K750011	.	engineered_region	946	2965	.	+	0	ID=BBa_K750007;Name=BBa_K750007
BBa_K750011	.	engineered_region	946	2019	.	+	0	ID=BBa_K750006;Name=BBa_K750006;Parent=BBa_K750007
BBa_K750011	.	terminator	1891	2019	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750006
BBa_K750011	.	terminator	1979	2019	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750011	.	stem_loop	1986	2005	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750011	.	polyA_site	2006	2019	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750011	.	stop_codon	2012	2012	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750011	.	engineered_region	1979	2019	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750011	.	terminator	1891	1970	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750011	.	stem_loop	1902	1945	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750011	.	engineered_region	1891	1970	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750011	.	promoter	946	1075	.	+	0	ID=BBa_K206000;Name=BBa_K206000;Parent=BBa_K750006
BBa_K750011	.	operator	1006	1023	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K750011	.	promoter	946	1076	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K750011	.	operator	985	1002	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K750011	.	ribosome_entry_site	1084	1095	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K750006
BBa_K750011	.	conserved_region	1088	1091	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750011	.	CDS	1102	1857	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K750006
BBa_K750011	.	engineered_region	1102	1103	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K750011	.	engineered_tag	1858	1882	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K750011	.	CDS	1102	1851	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K750011	.	point_mutation	1593	1593	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K750011	.	point_mutation	1275	1275	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K750011	.	engineered_region	1102	1857	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K750011	.	CDS	2109	2828	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K750007
BBa_K750011	.	CDS	2109	2828	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K750011	.	ribosome_entry_site	2091	2102	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K750007
BBa_K750011	.	conserved_region	2095	2098	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K750011	.	terminator	2837	2965	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K750007
BBa_K750011	.	terminator	2925	2965	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K750011	.	stem_loop	2932	2951	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K750011	.	polyA_site	2952	2965	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K750011	.	stop_codon	2958	2958	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K750011	.	engineered_region	2925	2965	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K750011	.	terminator	2837	2916	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K750011	.	stem_loop	2848	2891	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K750011	.	engineered_region	2837	2916	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K750011	.	promoter	2028	2082	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K750007
BBa_K750011	.	promoter	2047	2052	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K750011	.	engineered_region	2028	2082	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K750011	.	non_covalent_binding_site	2028	2047	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K750011	.	start_codon	2080	2080	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K750011	.	promoter	2069	2074	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
>BBa_K750011
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagtcacacaggaaagtactaga
tgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatca
agtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgca
gaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgc
tgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgc
tgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacac
gctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttc
cttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatga
acagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctag
tgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaca
ttgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctt
tttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaa
aaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgc
ttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctg
atatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctat
agtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctcca
aatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcg
gaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccatt
aattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaaga
gaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgta
ctgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaatttt
aacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgca
agaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttca
ctggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaagg
tgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtc
actactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgcca
tgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtt
tgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaa
ttggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaa
ttagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgt
ccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtc
cttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttata