LOCUS BBa_K750012 2963 bp DNA linear 22-Nov-2024 DEFINITION TIME DELAY0.01:LuxI(RBS0.01)->LuxR->LuxPR->GFP ACCESSION BBa_K750012 VERSION BBa_K750012.1 FEATURES Location/Qualifiers promoter 1..131 /label=promoter misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 promoter 1..130 /label=BBa_K206000 RBS 139..149 /label=RBS-4\Weaker RBS 145..148 /label=RBS misc_feature 139..149 /label=BBa_B0033 RBS 139..149 /label=BBa_B0033 misc_feature 735..766 /label=LVA CDS 156..734 /label=luxI misc_feature 156..773 /label=BBa_C0061 misc_feature 774..798 /label=Help:Barcodes CDS 156..773 /label=BBa_C0061 stem_loop 818..861 /label=stem_loop misc_feature 807..886 /label=BBa_B0010 terminator 807..886 /label=BBa_B0010 stem_loop 902..921 /label=T7 TE misc_feature 928..928 /label=stop polyA_site 922..935 /label=polya misc_feature 895..935 /label=BBa_B0012 terminator 895..935 /label=BBa_B0012 terminator 807..935 /label=BBa_B0015 misc_feature 1..935 /label=BBa_K750005 promoter 944..1074 /label=promoter misc_feature 983..1000 /label=AraI1 misc_feature 1004..1021 /label=AraI2 promoter 944..1073 /label=BBa_K206000 misc_feature 1086..1089 /label=conserved RBS 1082..1093 /label=BBa_B0034 misc_feature 1100..1101 /label=prefix misc_feature 1273..1273 /label=T misc_feature 1591..1591 /label=A CDS 1100..1849 /label=luxR misc_feature 1100..1855 /label=BBa_C0062 misc_feature 1856..1880 /label=Help:Barcodes CDS 1100..1855 /label=BBa_C0062 stem_loop 1900..1943 /label=stem_loop misc_feature 1889..1968 /label=BBa_B0010 terminator 1889..1968 /label=BBa_B0010 stem_loop 1984..2003 /label=T7 TE misc_feature 2010..2010 /label=stop polyA_site 2004..2017 /label=polya misc_feature 1977..2017 /label=BBa_B0012 terminator 1977..2017 /label=BBa_B0012 terminator 1889..2017 /label=BBa_B0015 misc_feature 944..2017 /label=BBa_K750006 misc_feature 2026..2045 /label=LuxR/HSL promoter 2045..2050 /label=-35 promoter 2067..2072 /label=-10 misc_feature 2078..2078 /label=start misc_feature 2026..2080 /label=BBa_R0062 promoter 2026..2080 /label=BBa_R0062 misc_feature 2093..2096 /label=conserved RBS 2089..2100 /label=BBa_B0034 CDS 2107..2826 /label=GFP protein CDS 2107..2826 /label=BBa_E0040 stem_loop 2846..2889 /label=stem_loop misc_feature 2835..2914 /label=BBa_B0010 terminator 2835..2914 /label=BBa_B0010 stem_loop 2930..2949 /label=T7 TE misc_feature 2956..2956 /label=stop polyA_site 2950..2963 /label=polya misc_feature 2923..2963 /label=BBa_B0012 terminator 2923..2963 /label=BBa_B0012 terminator 2835..2963 /label=BBa_B0015 misc_feature 944..2963 /label=BBa_K750007 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagtc acacaggact actagatgac tataatgata aaaaaatcgg 181 attttttggc aattccatcg gaggagtata aaggtattct aagtcttcgt tatcaagtgt 241 ttaagcaaag acttgagtgg gacttagttg tagaaaataa ccttgaatca gatgagtatg 301 ataactcaaa tgcagaatat atttatgctt gtgatgatac tgaaaatgta agtggatgct 361 ggcgtttatt acctacaaca ggtgattata tgctgaaaag tgtttttcct gaattgcttg 421 gtcaacagag tgctcccaaa gatcctaata tagtcgaatt aagtcgtttt gctgtaggta 481 aaaatagctc aaagataaat aactctgcta gtgaaattac aatgaaacta tttgaagcta 541 tatataaaca cgctgttagt caaggtatta cagaatatgt aacagtaaca tcaacagcaa 601 tagagcgatt tttaaagcgt attaaagttc cttgtcatcg tattggagac aaagaaattc 661 atgtattagg tgatactaaa tcggttgtat tgtctatgcc tattaatgaa cagtttaaaa 721 aagcagtctt aaatgctgca aacgacgaaa actacgcttt agtagcttaa taactctgat 781 agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 841 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 901 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagacattga ttatttgcac 961 ggcgtcacac tttgctatgc catagcaaga tagtccataa gattagcgga tcctacctga 1021 cgctttttat cgcaactctc tactgtttct ccataccgtt tttttgggct agctactaga 1081 gaaagaggag aaatactaga tgaaaaacat aaatgccgac gacacataca gaataattaa 1141 taaaattaaa gcttgtagaa gcaataatga tattaatcaa tgcttatctg atatgactaa 1201 aatggtacat tgtgaatatt atttactcgc gatcatttat cctcattcta tggttaaatc 1261 tgatatttca atcctagata attaccctaa aaaatggagg caatattatg atgacgctaa 1321 tttaataaaa tatgatccta tagtagatta ttctaactcc aatcattcac caattaattg 1381 gaatatattt gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa 1441 aacatcaggt cttatcactg ggtttagttt ccctattcat acggctaaca atggcttcgg 1501 aatgcttagt tttgcacatt cagaaaaaga caactatata gatagtttat ttttacatgc 1561 gtgtatgaac ataccattaa ttgttccttc tctagttgat aattatcgaa aaataaatat 1621 agcaaataat aaatcaaaca acgatttaac caaaagagaa aaagaatgtt tagcgtgggc 1681 atgcgaagga aaaagctctt gggatatttc aaaaatatta ggttgcagtg agcgtactgt 1741 cactttccat ttaaccaatg cgcaaatgaa actcaataca acaaaccgct gccaaagtat 1801 ttctaaagca attttaacag gagcaattga ttgcccatac tttaaaaatt aataacactg 1861 atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 1921 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 1981 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagacctg taggatcgta 2041 caggtttacg caagaaaatg gtttgttata gtcgaataaa tactagagaa agaggagaaa 2101 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta 2161 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca 2221 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca 2281 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg 2341 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata 2401 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc 2461 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga 2521 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag 2581 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta 2641 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac 2701 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg 2761 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa 2821 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 2881 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 2941 gggtgggcct ttctgcgttt ata //